OPTICAL IMAGING OF TUMOR ANGIOGENESIS AND CHEMOTHERAPY

肿瘤血管生成和化疗的光学成像

• 批准号: 8169462
• 负责人: ALBERT Edward CERUSSI
• 金额: $ 0.21万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169462
• 关键词: Animal Cancer Model; Animal Model; Biochemistry; Biological; Cisplatin; Clinical; Computer Retrieval of Information on Scientific Projects Database; Cyclophosphamide; Detection; Fatty acid glycerol esters; Female; Funding; Gases; Grant; Hemoglobin; Histology; Image; Institution; Intervention; Lighting; Mammary Neoplasms; Mammary gland; Measurement; Modeling; Monitor; Mutate; Optics; Pattern; Phase; Property; Rattus; Research; Research Personnel; Resources; Source; Technology; Tissues; Transgenic Mice; Tumor Angiogenesis; United States National Institutes of Health; Water; chemotherapy; instrument; interest; neoplastic cell; optical imaging; tumor; tumor growth; Animal Cancer Model; Animal Model; Biochemistry; Biological; Cisplatin; Clinical; Computer Retrieval of Information on Scientific Projects Database; Cyclophosphamide; Detection; Fatty acid glycerol esters; Female; Funding; Gases; Grant; Hemoglobin; Histology; Image; Institution; Intervention; Lighting; Mammary Neoplasms; Mammary gland; Measurement; Modeling; Monitor; Mutate; Optics; Pattern; Phase; Property; Rattus; Research; Research Personnel; Resources; Source; Technology; Tissues; Transgenic Mice; Tumor Angiogenesis; United States National Institutes of Health; Water; chemotherapy; instrument; interest; neoplastic cell; optical imaging; tumor; tumor growth

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Modulated imaging (MI) is a recent technology that uses patterned illumination and non-contact detection to obtain quantitative subsurface images of tissue optical properties over a wide field-of-view. MI is well-suited for the non-invasive study of cancer animal models that can be used to understand the relationship between tissue optical properties and tissue histology and biochemistry. In particular, we are interested in the biological mechanisms of tumor angiogenesis and their alterations from chemotherapy. Two different animal models were studied. In the first model, 13762 MAT-III rat mammary tumor cells were inoculated in the mammary fat pad of female Fisher 344 rats (~160g). The MI instrument acquired tissue optical property images during both tumor growth and treatment (Cyclophosphamide) phases. From these images, the tissue concentrations of oxy-hemoglobin, doexy-hemoglobin, and water were quantified. Tissue scattering parameters were also determined. In a second model we imaged p53/brca1 mutated transgenic mouse tumors under cisplatin treatment (5mg/kg daily for a week). During the MI measurements, hyperoxic gas intervention was also applied as a contrast to detect tumors and to monitor chemotherapeutic effects. These studies are so far very preliminary and the results obtained from MI need to be confirmed by immunohistochemical analysis. However, we have found great potential from the results of these animal models that can be applied to clinical cases.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 调制成像（MI）是一项最新技术，它使用图案化的照明和非接触式检测来在广泛的视野上获得组织光学特性的定量地下图像。 MI非常适合对癌症动物模型的非侵入性研究，这些研究可用于了解组织光学特性与组织组织学和生物化学之间的关系。特别是，我们对肿瘤血管生成的生物学机制感兴趣及其对化疗的改变。 研究了两种不同的动物模型。在第一个模型中，将13762 Mat-III大鼠乳腺肿瘤细胞接种在雌性Fisher 344大鼠（〜160G）的乳腺脂肪垫中。 MI仪器在肿瘤生长和治疗（环磷酰胺）阶段都获得了组织光学特性图像。从这些图像中，量化了氧血红蛋白，doexy-Hemoglobin和水的组织浓度。 还确定了组织散射参数。在第二个模型中，我们在顺铂治疗下成像P53/BRCA1突变的转基因小鼠肿瘤（每天5mg/kg一周）。在MI测量过程中，还采用了高氧气干预措施是检测肿瘤和监测化学治疗作用的对比。 到目前为止，这些研究非常初步，需要通过免疫组织化学分析来确认从MI获得的结果。但是，我们从这些动物模型的结果中发现了巨大的潜力，可以应用于临床病例。