TOMOGRAPHY TRAINING OF VISITORS TO THE LAB

实验室访客的断层扫描培训

• 批准号: 8170826
• 负责人: CINDY L SCHWARTZ
• 金额: $ 2.49万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170826
• 关键词: Amber; Antibodies; Austria; Computer Retrieval of Information on Scientific Projects Database; Computer software; Computer-Assisted Image Analysis; Cytoskeleton; Data; Data Set; Epithelial Cells; Fishes; Fission Yeast; Freezing; Funding; Germany; Grant; Hair Cells; Image; Image Analysis; Institutes; Institution; Israel; Laboratories; Learning; Mediating; Meiosis; Methods; Microscope; Microtubules; Modeling; Neurons; Plants; Plastic Embedding; Plastics; Preparation; Programmed Learning; Research; Research Personnel; Resolution; Resources; Rotation; Series; Source; Specimen; Stereocilium; Students; Training; United States National Institutes of Health; Universities; Visit; Wolves; Yeasts; coping; graduate student; neonatal Fc receptor; programs; reconstruction; spindle pole body; tomography

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This year our facility hosted a total of 15 groups of visitors, each of whom spent one week or more to learn various methods of specimen preparation, and/or to collect tilt series data and learn computer-assisted image analysis. This year, 7 visitors and students came specifically to collect cryo-tilt series and to learn the cryotomography methods developed in our lab. Herbert Poltzhofer and Guenter Resch (IMBA Vienna, Austria) visited the lab on two separate occasions to collect tilt series of frozen-hydrated whole mounts of fish keratocytes. Daniela Nicastro (Brandeis Univ.) spent three days collecting tilt series of isolated, plunge frozen microtubules decorated with klp 6. Dilem Hizlan (EMBL, Heidelberg, Germany) spent a week in the lab imaging isolated, plunge frozen microtubules with centralspindlin. In addition, three graduate students in MCDB, (Amber Rex, Julia Cope, Ian Ross, and Nesia Zurek) did rotations in the 3D lab to learn cryomicroscopy, cryotomography and image analysis. A fourth rotation student, Ian Ross, learned tomography of plastic-embedded meiotic yeast spindle pole bodies. Eight visitors came to collect data from plastic sections on the F30 microscope and/or to learn the various methods of 3D reconstruction using the IMOD software package. Each group was able to master aspects of the IMOD software package and has gone on to install the software in their own laboratories. Dr. Wanzhong He (CalTech University) spent 5 days collecting tilt series data on the F30 and learning the programs to study FcRn-mediated antibody transport across epithelial cells. Details of this project can be found in the Research Highlights. Dr. Hildur Palsdottir (Lawrence Berkeley Lab) spent three days collecting tilt series data of hair cell stereocilia. Helio Roque (EMBL, Heidelberg), collected montage tilt series data on the F20 and learned the IMOD modeling program and programs for tomographic reconstruction of the microtubule cytoskeleton in S. pombe. Mitusuko Hayashi (Yale University) collected tilt series data on the F30 microscope of neurons. Endre Majorovits (Oxford University) spent a week collecting tilt series data and learning the image analysis software for tomographic reconstruction and modeling. Ichirou Karahara (Univ. Toyama) collected tilt series data on the F30 microscope of plant cytoskeleton. Sharon Wolf (Weizmann Institute, Israel) spent a day with Cindi Schwartz learning details of the Serial EM image acquisition software for high-resolution data sets.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 今年，我们的设施总共托管了15个访客，每个访客都花了一个星期或更长时间来学习各种标本准备方法，和/或收集倾斜系列数据并学习计算机辅助的图像分析。 今年，有7位访客和学生专门收集了冷冻倾斜系列，并学习了我们实验室中开发的冷冻理学方法。赫伯特·波尔茨霍夫（Herbert Poltzhofer）和吉恩特（Guenter Resch）（奥地利IMBA维也纳）在两次单独的场合访问了实验室，以收集一系列冷冻水的鱼角膜细胞。 Daniela Nicastro（Brandeis Univ。）花了三天的时间收集倾斜系列孤立的，垂坠的冰冻的微管，装饰有KLP6。DilemHizlan（Embl，Embl，Heidelberg，Heidelberg，Germany，德国）在实验室中度过了一周，在实验室中，孤立的，Plunge Frozen Frozen Microtubules与Centrralspindlin。此外，MCDB中的三名研究生（Amber Rex，Julia Cope，Ian Ross和Nesia Zurek）在3D实验室进行了旋转，以学习冷冻显微镜，冷冻理学和图像分析。第四个旋转学生伊恩·罗斯（Ian Ross）学习了塑料包裹的减数分裂酵母纺锤体的断层扫描。八名访问者来收集F30显微镜上的塑料部分的数据和/或使用IMOD软件包学习3D重建的各种方法。每个组都能够掌握IMOD软件包的方面，并继续将软件安装在自己的实验室中。 Wanzhong He博士（加州大学）花了5天的时间收集有关F30的倾斜系列数据，并学习了研究FCRN介导的抗体转运的程序。该项目的详细信息可以在研究亮点中找到。 Hildur Palsdottir博士（Lawrence Berkeley Lab）花了三天时间收集毛细胞立体胶质的倾斜系列数据。 Helio Roque（EMBL，Heidelberg）收集了F20上的蒙太奇倾斜系列数据，并学习了S. Pombe中微管细胞骨架的层析成像重建程序的IMOD建模程序和程序。 Mitusuko Hayashi（耶鲁大学）收集了有关神经元F30显微镜的倾斜系列数据。 Endre Majorovits（牛津大学）花了一个星期来收集倾斜系列数据并学习用于层析成像重建和建模的图像分析软件。 Ichirou Karahara（Toyama Univ）收集了有关植物细胞骨架的F30显微镜的倾斜系列数据。 Sharon Wolf（以色列韦兹曼研究所）在Cindi Schwartz度过了一天，学习了用于高分辨率数据集的串行EM图像采集软件的详细信息。