Multimodality Optical Imaging Tools for Cocaine Studies

用于可卡因研究的多模态光学成像工具

• 批准号: 7935286
• 负责人: Congwu Du
• 金额: $ 50万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7935286
• 关键词: Acute; Address; Animals; Area; Biomedical Engineering; Blood Vessels; Blood Volume; Brain; Brain imaging; Brain region; Calcium; Calcium Signaling; Cell physiology; Cerebrovascular Circulation; Cerebrum; Chronic; Cocaine; Cocaine Abuse; Cocaine Dependence; Communities; Detection; Development; Drug Addiction; Drug abuse; Education; Event; Fluorescence; Foundations; Frequencies; Funding; Future; Goals; Grant; Hemoglobin; Human; Image; Imaging Device; Imaging technology; Lasers; Legal patent; Maps; Measurement; Monitor; Neurons; Neurosciences; Nucleus Accumbens; Optical Coherence Tomography; Optics; Pharmaceutical Preparations; Physics; Physiological; Positioning Attribute; Postdoctoral Fellow; Probability; Ramp; Rattus; Reaction Time; Research; Resolution; Rodent; Solid; Structure; Techniques; Technology; Testing; Three-Dimensional Image; Three-Dimensional Imaging; Time; Tissues; Toxic effect; Translational Research; Validation; addiction; base; bioimaging; cocaine exposure; digital; digital imaging; fluorescence imaging; graduate student; hemodynamics; in vivo; insight; instrumentation; lens; multimodality; next generation; novel; novel strategies; optical imaging; public health relevance; repaired; response; spatiotemporal; technology development; tool development; treatment strategy

DESCRIPTION (provided by applicant): This application entitled "Multimodality Optical Imaging Tools for Cocaine Studies" will address the broad Challenge Area (06: Enabling Technologies) and Specific Challenge topic (06-DA-102: Tool Development for the Neurosciences). The central goal of this proposal is to develop novel imaging tools that will provide new insights into the cascading events resulting from chronic cocaine exposure, in particular, the toxic effects on cortical and subcortical brain regions and including neurovascular changes. One of the big challenges when imaging the responses of the rodent brain to drugs (i.e., cocaine) has been to distinguish vascular from neuronal effects. We recently developed and tested a novel dual wavelength laser speckle imaging (DWLSI) tool that enables, for the first time, simultaneous measurements of cerebral blood flow (CBF), blood volume and tissue hemoglobin oxygenation, which serves as marker of tissue activity at high spatiotemporal resolutions (30¿m, 10Hz) and over a relatively large field of view (FOV, 4x5mm2). Moreover, by co-registering with digital-frequency-ramping optical coherence tomography (DOCT) we can obtain an in vivo quantitative 3D image of the CBF network. In parallel we also developed intracellular calcium imaging using Rhod2-fluorescence technique, which serves as a marker of neuronal activity. In this Challenge Grant, we propose to develop a multimodality simultaneous imaging platform by combining DWLSI, DOCT and calcium fluorescence imaging to study the effects of acute and chronic cocaine exposures on rodent brain. By further integrating this technique with our micro relay lens (F300¿mx24mm), we will be able, for the first time, to expand our multimodality imaging capabilities to study cocaine effects in deep brain regions in vivo. The Specific Aims are: (1) to develop multimodality imaging platform to study cocaine effects on cortical brain; (2) to develop microprobe-based multimodality imaging platform for subcortical brain imaging. Successful development and validation of the proposed multimodality optical techniques will permit simultaneous imaging of vascular and cellular functions and their real-time responses to cocaine challenge in cortical and subcortical brain in vivo, thus enabling distinction and detailed study of neuronal and vascular changes. These proposed technological developments will have significantly impact on the biomedical imaging, neuroscience and drug abuse and translational research communities. In addition, Challenge grant funds for this project will create 3 research positions for 1 postdoctoral fellow and 2 graduate student, which will accelerate the pace of new imaging technology development and enhance the academic education for next generation on biomedical engineering and physics to bridge instrumentation development for neuroscience to translational research on drug abuse and addiction. PUBLIC HEALTH RELEVANCE: This application entitled "Multimodality Optical Imaging Tools for Cocaine Studies" will address the broad Challenge Area (06: Enabling Technologies) and specific Challenge topic (06-DA-102: Tool Development for the Neurosciences). The central goal of this proposal is to develop novel imaging tools that will provide new insights into the cascading events resulting from chronic cocaine exposure, in particular, the toxic effects on cortical and subcortical brain regions and including neurovascular changes. In this Challenge Grant, we propose to develop a multimodality simultaneous imaging platform by combining DWLSI, DOCT and calcium fluorescence imaging to study the effects of acute and chronic cocaine exposures to the rodent brain. In addition, by integrating this technique with our micro relay lens we will be able to expand our imaging capabilities to study cocaine effects in deep brain regions in vivo. Successful development and validation of the proposed techniques will permit simultaneous imaging of vascular and cellular functions and their real-time responses to cocaine challenge in cortical and subcortical brain in vivo, thus enabling distinction and detailed study of neuronal and vascular changes. The proposed technological developments will have significantly impact on the biomedical imaging, neuroscience and drug abuse and translational research communities. In addition, Challenge grant funds for this project will create 3 research positions for post-doctor fellow and graduate students, which will enhance the academic education for next generation on Biomedical Engineering and Physics to bridge instrumentation development for neuroscience to translational research on drug abuse and addiction.

描述（由申请人提供）：本申请题为“用于可卡因研究的多模态光学成像工具”，将解决广泛的挑战领域（06：支持技术）和特定挑战主题（06-DA-102：神经科学中心的工具开发）。该提案的目标是开发新颖的成像工具，为长期接触可卡因引起的级联事件提供新的见解，特别是对皮质和皮质下大脑区域的毒性作用以及对啮齿动物大脑对药物（即可卡因）的反应进行成像时，最大的挑战之一是区分血管效应和神经元效应。我们最近开发并测试了一种新型双波长激光散斑成像 (DWLSI) 工具。首次能够同时测量脑血流量（CBF）、血容量和组织血红蛋白氧合，作为高时空分辨率（30¿ m，10Hz）和相对较大的视场（FOV，4x5mm2）此外，通过与数字频率斜坡光学相干断层扫描（DOCT）共同配准，我们可以获得CBF网络的体内定量3D图像。与此同时，我们还使用 Rhod2 荧光技术开发了细胞内钙成像，该技术可作为神经活动的标记。在本次挑战资助中，我们建议通过结合开发多模态同步成像平台。 DWLSI、DOCT 和钙荧光成像，用于研究急性和慢性可卡因暴露对啮齿动物大脑的影响 通过进一步将该技术与我们的微型中继镜头 (F300¿mx24mm) 相结合，我们将首次能够扩展我们的研究范围。多模态成像能力研究可卡因对大脑深层区域的影响，具体目标是：（1）开发多模态成像平台来研究可卡因对大脑皮层的影响；（2）开发基于微探针的技术。用于皮层下脑成像的多模态成像平台。所提出的多模态光学技术的成功开发和验证将允许对皮层和皮层下脑中的血管和细胞功能及其对可卡因挑战的实时反应进行同步成像，从而能够进行区分和详细研究。这些拟议的技术发展将对生物医学成像、神经科学和药物滥用以及转化研究界产生重大影响。此外，该项目的挑战拨款将为 1 名博士后研究员和 2 名博士后创造 3 个研究职位。研究生，这将加快新成像技术的开发步伐，并加强下一代生物医学工程和物理学的学术教育，以将神经科学仪器开发与药物滥用和成瘾转化研究联系起来。 公共健康相关性：这个题为“可卡因研究的多模态光学成像工具”的应用程序将解决广泛的挑战领域（06：支持技术）和特定挑战主题（06-DA-102：神经科学工具开发）的中心目标。该提案旨在开发新颖的成像工具，为长期接触可卡因引起的级联事件提供新的见解，特别是对皮质和皮质下大脑区域的毒性作用，包括在这项挑战资助中，我们建议通过结合 DWLSI、DOCT 和钙荧光成像来开发一种多模态同步成像平台，以研究急性和慢性可卡因暴露对啮齿动物大脑的影响。此外，将该技术与我们的研究相结合。微中继透镜，我们将能够扩展我们的成像能力，以研究可卡因在体内深部大脑区域的作用，所提出的技术的成功开发和验证将允许对血管和细胞功能及其对可卡因挑战的实时反应进行同步成像。皮质和皮质下脑从而能够对神经和血管变化进行区分和详细研究。拟议的技术发展将对生物医学成像、神经科学和药物滥用以及转化研究界产生重大影响。此外，该项目的挑战拨款将创建 3 项研究。博士后和研究生职位，这将加强下一代生物医学工程和物理学的学术教育，将神经科学仪器开发与药物滥用和成瘾转化研究联系起来。