Formation of the Enamel-Dentin Interface

牙釉质-牙本质界面的形成

基本信息

批准号：
7782760
负责人：
ELIA BENIASH
金额：
$ 26.93万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-02-15 至 2013-01-31
项目状态：
已结题

项目摘要

The purpose of the proposed studies is to improve our understanding of the basic physico-chemical mechanisms of initial enamel mineralization and formation of the dentin-enamel interface. Enamel mineralization starts at the surface of a layer of mineralized dentin, which may play an important role in initial enamel formation. Dentin and enamel are two mineralized tissues with strikingly different mechanical and structural properties that normally perform jointly for tens of years, without failure. Such an outstanding mechanical endurance requires an extraordinarily strong bond between these two tissues. Studies of tooth- related genetic disorders and knockout animals demonstrate that the correct formation of the dentin-enamel interface is essential for the proper tooth function. The problem of interface stability is also very important with respect to tissue repair, where often implant failure occurs due to a weak interface between tissues and repair materials. It is likely that interactions between dentin and enamel tissues during initial mineralization play an important role in the proper formation of this critically important interface. We hypothesize that the onset of mineralization at the boundary between enamel and dentin is a highly integrated and specialized process, essential for the proper formation of this mechanically robust interface. Wealso hypothesize that the mechanism of initial enamel formation at this interface is different from other stages of amelogenesis. We have identified three major factors that distinguish initial enamel formation from later stages of amelogenesis: 1) the presence of dentin mineral at the site of initial enamel formation; 2) the presence of transient mineral phases; and 3) a unique macromolecular composition at the site of initial enamel formation. Thus, we propose a comprehensive study of the formation of the dentin-enamel interface using a variety of in vivo and in vitro approaches to gain a better understanding of basic mechanisms of initial enamel formation. Specifically, we propose in Aim1: To study the interactions between dentin and enamel crystals at the site of initial enamel mineralization, with special emphasis on the epitaxial relationship between dentin and enamel crystals; in Aim 2: To study the role of transient amorphous mineral phases in initial stages of enamel formation; and in Aim 3: To study the macromolecular composition at the initial enamel mineralization site with special emphasis on protein-mineral interactions, and to elucidate the possible functions of these macromolecules and supra-molecular assemblies in initial enamel formation using a mechanistic approach, in a series of in vitro mineralization experiments. We expect that the results of this study will improve our knowledge of the basic processes involved in the formation of the dentin-enamel interface that will hopefully lead to the development of new advanced materials and/or procedures for mineralized tissue repair.

拟议研究的目的是提高我们对基本物理化学的理解初始牙釉质矿化和牙本质-牙釉质界面形成的机制。搪瓷矿化开始于矿化牙本质层的表面，这可能在初始阶段发挥重要作用。牙釉质的形成。牙本质和牙釉质是两种矿化组织，具有显着不同的机械和性能通常可以共同发挥数十年而不失效的结构特性。如此出色的机械耐力需要这两个组织之间具有非常牢固的结合。牙齿研究—— 相关的遗传性疾病和基因敲除动物证明牙本质釉质的正确形成界面对于牙齿的正常功能至关重要。接口稳定性问题也很重要在组织修复方面，由于组织和组织之间的界面薄弱，常常会发生植入失败修复材料。初始矿化过程中牙本质和牙釉质组织之间可能发生相互作用在这个极其重要的界面的正确形成中发挥着重要作用。我们假设牙釉质和牙本质之间的边界矿化的开始是一个高度整合和专门的工艺，对于正确形成这种机械坚固的界面至关重要。韦尔索斯假设该界面的初始牙釉质形成机制与其他界面不同釉质形成的阶段。我们已经确定了区分初始牙釉质形成的三个主要因素从釉质形成的后期阶段：1）在最初的牙釉质形成部位存在牙本质矿物质； 2）存在瞬时矿物相； 3）在初始位点具有独特的大分子组成牙釉质的形成。因此，我们建议对牙本质-牙釉质界面的形成进行全面研究使用各种体内和体外方法来更好地了解基本机制牙釉质的初步形成。具体来说，我们在目标 1 中提出：研究牙本质和牙本质之间的相互作用最初牙釉质矿化部位的牙釉质晶体，特别强调外延关系牙本质和牙釉质晶体之间；目标 2：研究瞬态非晶矿物相在牙釉质形成的初始阶段；目标 3：研究初始时的大分子组成牙釉质矿化位点，特别强调蛋白质-矿物质相互作用，并阐明这些大分子和超分子组装体在初始牙釉质形成中的可能功能使用机械方法进行一系列体外矿化实验。我们期望结果这项研究将提高我们对形成的基本过程的了解牙本质-牙釉质界面有望促进新的先进材料的开发和/或矿化组织修复程序。