EXTRACELLULAR MATRIX ON B-CASEIN GENE EXPRESS IN NORMAL MAMMARY EPITHELIAL CELL

正常乳腺上皮细胞中 B-酪蛋白基因表达的细胞外基质

• 批准号: 7957446
• 负责人: MINA BISSELL
• 金额: $ 1.28万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957446
• 关键词: Basement membrane; Biochemical; Biological Assay; Biological Models; Breast; Caseins; Cell Nucleus; Cells; Communication; Computer Retrieval of Information on Scientific Projects Database; Development; Disease; Environment; Epithelial Cells; Extracellular Matrix; Funding; Gene Expression; Genes; Genetic Materials; Grant; Institution; Intervention; Laminin; Mammary gland; Microscopy; Organism; Regulation; Research; Research Personnel; Resources; Role; Source; Structure; Techniques; United States National Institutes of Health; X-Ray Tomography; breast tumorigenesis; extracellular; tumor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cell development in higher organisms is controlled in part by communication between the nucleus and the surrounding extracellular environment, and accumulating evidence shows that changes in the structure of a cell''''s genetic material are central to the regulation of this communication. The inability of the extracellular environment to communicate properly with the nucleus is one of the hallmarks of tumor development. We have developed a model system in which normal mammary epithelial cells respond to a physiologically relevant three-dimensional, laminin-rich basement membrane (IrBM) by undergoing a functional differentiation. This assay will be used to determine how the extracellular environment influences the structure and organization of a cell's genetic material in a manner that regulates the expression of specific genes. To identify these changes, we will employ a combination of biochemical and microscopy techniques. Characterizing the role of the extracellular environment on mammary cell development will contribute towards a better understanding of normal breast cell development and gene expression and may also have implications for breast tumorigenesis and disease intervention.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 高等生物体的细胞发育部分由细胞核与周围环境之间的通讯控制 细胞外环境，并且越来越多的证据表明细胞遗传物质结构的变化是 对此通信的监管的核心。细胞外环境无法正常沟通 与细胞核的结合是肿瘤发展的标志之一。我们开发了一个模型系统，其中正常 乳腺上皮细胞对生理相关的三维、富含层粘连蛋白的基底膜（IrBM）做出反应 通过进行功能分化。该测定将用于确定细胞外环境如何 以调节特定基因表达的方式影响细胞遗传物质的结构和组织 基因。为了识别这些变化，我们将结合使用生化和显微镜技术。 表征细胞外环境对乳腺细胞发育的作用将有助于更好地研究 了解正常乳腺细胞发育和基因表达，也可能对乳腺产生影响 肿瘤发生和疾病干预。