Muscle Regrowth during Physical Rehabilitation and Amino Acid Supplementation

物理康复和氨基酸补充期间的肌肉再生

基本信息

批准号：
7673325
负责人：
Hans Christian Dreyer
金额：
$ 12.83万
依托单位：
UNIVERSITY OF OREGON
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-09-01 至 2013-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7673325
关键词：
Acute Address Adopted Amino Acids Area Arthritis Arts Atrophic Attenuated Award Catabolism Cells Clinical Research Data Development Elderly Environment Essential Amino Acids Event Funding Future Goals Grant Hand Ingestion Knee Learning Link Measurement Medical Medical Research Mentors Methodology Muscle Muscle Proteins Musculoskeletal Nutritional Operative Surgical Procedures Outcome Measure Performance Physical Function Physical therapy Physiological Placebos Population Protein Biosynthesis Proteins Recovery Regulation Rehabilitation Research Rehabilitation therapy Research Research Design Research Infrastructure Research Personnel Research Project Grants Research Training Scientist Signal Transduction Skeletal Muscle Stress Supervision Supplementation System Techniques Testing Texas Training Training and Education Translational Research United States National Institutes of Health Universities base bone career career development functional outcomes human FRAP1 protein improved knee pain knee replacement arthroplasty mTOR Signaling Pathway muscle form muscle metabolism muscle strength novel protein metabolism quadriceps muscle rehabilitation science rehabilitation strategy research study responsible research conduct skills stable isotope

项目摘要

DESCRIPTION (provided by applicant): The purpose of this proposal is to further the candidate?s scientific development in translational and clinical research and to make possible his transition to become an independent translational scientist in rehabilitation. The University of Oregon provides an outstanding academic and research environment and infrastructure to facilitate the candidate?s education and training in the conduct of translational research. Career Goals: This award will support the candidate?s successful transition from a junior researcher into a mid level independent investigator by providing an array of formal and informal career development activities, and a research project for hands-on learning and acquisition of essential preliminary data under the supervision of an exceptional mentoring team. The specific short-term career goals will include training in muscle metabolism, stable isotope techniques and rehabilitation research; training in the responsible conduct of research and other standard scientific skills; performance of a research study for the acquirement of practical clinical research skills and of preliminary data for future research applications in rehabilitation science. The long-term scientific goal is for the candidate to become an expert in skeletal muscle metabolism and rehabilitation with continuous funding for medical research. Research Plan: The general hypothesis is that in older adults muscle regrowth after an acute musculoskeletal stress will be positively influenced by traditional physical rehabilitation, and further enhanced by nutritional supplementation. Using state-of-the-art stable isotope methodologies for the study of muscle metabolism and methodologies for the measurement of cell signaling, we will test the following specific hypotheses: 1a) To determine the effects of ischemia-reperfusion injury on cell signaling and gene expression in skeletal muscle during TKR surgery; 1b) To determine if acute changes in muscle cell signaling and gene expression correlate with muscle atrophy measured 2 weeks later by MRI; 2) To determine if muscle protein synthesis is acutely increased with the ingestion of EAA following TKA; 3) To determine the effects of EAA Supplementation during PT rehabilitation on muscle mass, strength, and function before and after TKA. Public Benefit: This research will focus rehabilitation efforts on specific and currently unresolved mechanisms responsible for muscle loss following total knee replacement in older adults. While knee pain due to bone arthritis is often alleviated after knee replacement, complete return of physical function and independence is difficult to achieve. This research will help to restore physical function and independence in the rapidly growing population of older adults with knee arthritis.

描述（由申请人提供）：该提案的目的是进一步转化和临床研究中的科学发展，并使他的过渡成为康复的独立转化科学家。俄勒冈大学提供了出色的学术和研究环境和基础设施，以促进候选人的教育和培训。职业目标：该奖项将通过提供一系列正式和非正式的职业发展活动，以及在一个杰出的指导团队的监督下，通过提供一系列正式和非正式的职业发展活动，并获得一系列正式和非正式职业发展活动的候选人的成功过渡到中级独立研究者。特定的短期职业目标将包括肌肉代谢，稳定同位素技术和康复研究的培训；负责研究和其他标准科学技能的培训；一项研究的研究，以获取实用临床研究技能和初步数据，以恢复科学领域的研究应用。长期的科学目标是让候选人成为骨骼肌肉代谢和康复的专家，并持续进行医学研究资金。研究计划：一般假设是，在老年人中，急性肌肉骨骼应激后的肌肉再生将受到传统身体康复的积极影响，并通过补充营养而进一步增强。使用最先进的同位素方法学来研究肌肉代谢和测量细胞信号传导的方法，我们将测试以下特定假设：1a），以确定TKR手术过程中骨骼肌中缺血性灌注损伤对细胞信号传导和基因表达的影响； 1b）确定肌肉细胞信号传导和基因表达的急性变化是否与2周后通过MRI测量的肌肉萎缩相关； 2）确定肌肉蛋白合成是否随着TKA后的EAA摄入而急剧增加； 3）确定PT康复过程中补充EAA对TKA前后肌肉质量，强度和功能的影响。公共利益：这项研究将把康复工作集中在老年人全膝关节置换后，造成肌肉损失的特定和目前未解决的机制。虽然骨关节炎引起的膝盖疼痛通常会在膝盖置换后得到缓解，但很难完全恢复身体机能和独立性。这项研究将有助于恢复膝盖关节炎的老年人迅速增长的人口的身体机能和独立性。