Aedes anti-dengue immunity

伊蚊抗登革热免疫力

• 批准号: 7914347
• 负责人: George Dimopoulos
• 金额: $ 41万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-15 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7914347
• 关键词: Aedes; Antiviral Agents; Biological Assay; Bioterrorism; Categories; Controlled Study; Culicidae; Dengue; Dengue Virus; Development; Dissection; Gene Expression; Gene Silencing; Goals; Immune; Immune response; Immune system; Immunity; Immunologic Surveillance; Infection; Infection Control; Knowledge; Link; Mediating; Methods; Molecular; Molecular Biology; National Institute of Allergy and Infectious Disease; Natural Immunity; Pathway interactions; Physiological; Process; Provider; Publishing; RNA Interference; Reporting; Research; Research Proposals; Serotyping; Signal Pathway; Specificity; Staging; System; Time; Tissues; Variant; Viral; Virus; Virus Diseases; Yellow Fever; base; combat; defense response; feeding; functional genomics; genome sequencing; insight; novel; pathogen; positional cloning; research study; response; tool; tool development; transmission process; vector; vector mosquito

Mosquitoes utilize a variety of strategies to combat pathogens, including viruses. The long term_ goal of the PIs research is to elucidate, at the molecular level, the immune responses of the mosquito vector Aedes aegypti to infection with a range of viral pathogens, including those that could be used as agents for bioterrorism. In recent years, dengue fever has emerged as one of the most serious vector borne viral diseases. The molecular biology of dengue virus propagation in the mosquito vector is largely unknown, but the genome sequence and reverse genetic and functional genomics tools that are now available provide a means of dissecting the mosquito’s anti viral defense systems in greater detail. In this proposal, we will apply these tools to characterize the Ae. aegypti mosquito’s innate immune defense against the dengue virus. We have already showed that the mosquito is employing the Toll and JAK STAT immune pathways to limit dengue virus infection. We will now expand on this finding and characterize the mosquito’s broader molecular immune responses to virus infection at multiple time points and tissues in Specific Aim 1. We will analyze the implication of the mosquito’s immune pathways in defense against dengue virus infection in a greater detail in Specific Aim 2. In addition to the basic knowledge generated by the proposed experiments, this project should also provide a variety of tools that can be used for the development of novel antiviral control strategies.

蚊子利用各种策略来打击包括病毒在内的病原体。 PIS研究的长期目标是在分子水平上阐明蚊子载体埃德斯埃及埃及埃及的免疫反应，以感染一系列病毒病原体，包括可以用作生物恐怖剂的药物。近年来，登革热已成为最严重的载体传播病毒疾病之一。蚊子载体中登革热病毒繁殖的分子生物学在很大程度上是未知的，但是基因组序列和反向遗传和功能基因组学工具现在可用来提供一种剖析蚊子的抗病毒防御系统的方法。在此提案中，我们将应用这些工具来表征AE。埃及蚊子对球迷的先天免疫防御。我们已经表明，蚊子正在采用Toll和Jak Stat Immune途径来限制风扇病毒感染。现在，我们将扩大这一发现并表征蚊子对多个时间点和特定目标中的时间点的更广泛的分子免疫反应1。我们将分析蚊子免疫途径在防御风扇感染中的免疫途径在防御风扇感染中的影响，以更详细的详细范围内，还可以提供详细的详细信息。除了拟议中，还可以为拟议的工具提供了一个多样性的工具。策略。

项目成果

The entomopathogenic fungus Beauveria bassiana activate toll and JAK-STAT pathway-controlled effector genes and anti-dengue activity in Aedes aegypti.

• DOI: 10.1016/j.ibmb.2011.11.005
• 发表时间: 2012-02
• 期刊: INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
• 影响因子: 3.8
• 作者: Dong, Yuemei;Morton, James C., Jr.;Ramirez, Jose Luis;Souza-Neto, Jayme A.;Dimopoulos, George
• 通讯作者: Dimopoulos, George

Aedes aegypti ML and Niemann-Pick type C family members are agonists of dengue virus infection.

• DOI: 10.1016/j.dci.2013.10.002
• 发表时间: 2014-03
• 期刊: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
• 影响因子: 2.9
• 作者: Jupatanakul, Natapong;Sim, Shuzhen;Dimopoulos, George
• 通讯作者: Dimopoulos, George

Transcriptomic profiling of diverse Aedes aegypti strains reveals increased basal-level immune activation in dengue virus-refractory populations and identifies novel virus-vector molecular interactions.

• DOI: 10.1371/journal.pntd.0002295
• 发表时间: 2013
• 期刊: PLoS neglected tropical diseases
• 影响因子: 3.8
• 作者: Sim S;Jupatanakul N;Ramirez JL;Kang S;Romero-Vivas CM;Mohammed H;Dimopoulos G
• 通讯作者: Dimopoulos G