Tandem TIme-of-Flight Mass Spectromer for Proteomics

用于蛋白质组学的串联飞行时间质谱仪

• 批准号: 7595388
• 负责人: Kermit King Murray
• 金额: $ 43.7万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595388
• 关键词: Alzheimer' s Disease; Arterial Fatty Streak; Basic Science; Biological Markers; Biological Sciences; Biomedical Research; Capillary Electrophoresis; Chemistry; Chimeric Proteins; Communities; Complement; Development; Disease; Doctor of Philosophy; Enzyme Inhibition; Enzymes; Funding; Genetic; Herpesvirus 1; Housing; Hybrids; Iron-Sulfur Proteins; Louisiana; Mass Spectrum Analysis; Membrane Fusion; Peptides; Porphyrins; Protein Analysis; Proteins; Proteomics; Public Health; Research; Research Infrastructure; Research Personnel; Resolution; Running; STAT protein; Schools; Scientist; Signal Transduction Pathway; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spottings; Time; United States National Institutes of Health; Universities; Veterinary Medicine; base; bioimaging; cancer therapy; college; combat; experience; instrument; liver metabolism; mass spectrometer; peptide analog; protein protein interaction; public health relevance; success; tool; Alzheimer' s Disease; Arterial Fatty Streak; Basic Science; Biological Markers; Biological Sciences; Biomedical Research; Capillary Electrophoresis; Chemistry; Chimeric Proteins; Communities; Complement; Development; Disease; Doctor of Philosophy; Enzyme Inhibition; Enzymes; Funding; Genetic; Herpesvirus 1; Housing; Hybrids; Iron-Sulfur Proteins; Louisiana; Mass Spectrum Analysis; Membrane Fusion; Peptides; Porphyrins; Protein Analysis; Proteins; Proteomics; Public Health; Research; Research Infrastructure; Research Personnel; Resolution; Running; STAT protein; Schools; Scientist; Signal Transduction Pathway; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spottings; Time; United States National Institutes of Health; Universities; Veterinary Medicine; base; bioimaging; cancer therapy; college; combat; experience; instrument; liver metabolism; mass spectrometer; peptide analog; protein protein interaction; public health relevance; success; tool

DESCRIPTION (provided by applicant): The instrument described in this application is a matrix-assisted laser desorption ionization (MALDI) tandem time-of-flight (TOF/TOF) mass spectrometer. The proposed instrument will be a part of the shared mass spectrometry user facility and housed in the College of Basic Sciences Department of Chemistry at Louisiana State University. This facility is staffed and supervised by Ph.D. scientists with extensive experience in mass spectrometry and separations. Access to this instrument will significantly enhance the quality, quantity and scope of a large number of NIH supported investigators. Specific projects that will be enhanced by access to this instrument include College of Basic Sciences, Biological Sciences Department: The instrument will be used in analysis of NO-modified iron-sulfur proteins (Ding), protein/protein interactions important in liver metabolism (Pr|fer), and the identification of proteins involved in STAT protein signal transduction pathways (Stephens). College of Basic Sciences, Chemistry Department: the MALDI TOF/TOF instrument will support the study of enzyme function through the development and application of capillary electrophoresis-based approaches for studying enzyme inhibition (Gilman), research in the development of anti-Alzheimer's peptide analogs (Hammer), the identification of MS/MS bacterial biomarkers (Murray), the study of porphyrin bioconjugates for bioimaging and cancer therapy (Vicente), and the structural analysis of proteins involved in atherosclerotic plaque formation (Warner). Louisiana State University School of Veterinary Medicine: The proposed instrument will be used to achieve maximum sequence coverage in a study of genetics and functions of the HSV-1 membrane fusion protein with MALDI run in parallel with ESI with LC separation of digested peptides (Kousoulas). The proposed instrument will complement a hybrid quadrupole TOF mass spectrometer already in the MS facility and will be able to leverage existing research infrastructure for LC-MALDI plate spotting. PUBLIC HEALTH RELEVANCE: Relevance Mass spectrometers are powerful tools of the analysis of biomolecules and access to them is essential for the success of biomedical research. The proposed instrument has unique capabilities of high mass resolution, large mass range, and high throughput that are not currently available to researchers at Louisiana State University. This instrument will allow NIH funded researchers at LSU to identify and quantify proteins and other biomacromolecules important in understanding and combating diseases and in protecting the public health. The instrument will be operated by skilled technicians, overseen by broadly represented committee and available for use by the entire LSU scientific community.

描述（由申请人提供）：本申请中描述的仪器是基质辅助激光解吸电离（MALDI）串联飞行时间（TOF/TOF/TOF）质谱仪。拟议的工具将成为共享质谱用户设施的一部分，并位于路易斯安那州立大学基础科学学院化学系。该设施由博士学位主持和监督。在质谱和分离方面拥有丰富经验的科学家。使用该工具将大大提高大量NIH支持研究人员的质量，数量和范围。可以通过使用该工具来增强的特定项目包括基础科学学院，生物科学系：该仪器将用于分析无修饰的铁硫蛋白（DING），在肝代谢（PR | FER）中很重要的蛋白质/蛋白质相互作用，以及涉及Stat蛋白质信号转换途径的蛋白质鉴定的蛋白质/蛋白质相互作用。基础科学学院化学系：MALDI TOF/TOF仪器将通过开发和应用基于毛细血管电泳的方法来研究酶抑制酶抑制（GILMAN）的研究，研究抗阿尔基氏症肽类模拟的抗alzheimer（Hammer）的开发，研究MS/MS MS Biomark（Mers Biomar）（MURRAIN）（MUR）。生物成像和癌症治疗（Vicente）的生物缀合物以及参与动脉粥样硬化斑块形成（Warner）的蛋白质的结构分析。路易斯安那州立大学兽医学院：在对HSV-1膜融合蛋白的遗传学和功能的研究中，该仪器将用于获得最大的序列覆盖率，其中MALDI与ESI同行与ESI同行，其LC分离消化肽（Kousoulas）。该拟议的仪器将补充MS设施中已经存在的混合四极杆质谱仪，并能够利用现有的研究基础设施来斑点板块。公共卫生相关性：相关性质谱仪是分析生物分子的强大工具，及其访问对于生物医学研究的成功至关重要。拟议的仪器具有高质量分辨率，大质量范围和高吞吐量的独特功能，目前是路易斯安那州立大学的研究人员可用的。该工具将允许NIH资助的LSU研究人员识别和量化蛋白质和其他生物大分子，这对于理解和打击疾病以及保护公共卫生很重要。该工具将由熟练的技术人员运营，由广泛代表的委员会监督，并由整个LSU科学界使用。