Development of remotely actuated deformable membranes for in situ mechanical testing of soft tissue

开发用于软组织原位机械测试的远程致动变形膜

• 批准号: 10708754
• 负责人: Andres Arrieta Diaz
• 金额: $ 18.17万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-23 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10708754
• 关键词: 3-Dimensional; Acute; Algorithms; Animal Disease Models; Animal Model; Animals; Architecture; Basic Science; Big Data; Biomechanics; Biomedical Engineering; Biomedical Research; Chronic; Cicatrix; Complex; Computer software; Connective and Soft Tissue; Contracture; Data; Data Set; Dermal; Development; Devices; Diagnostic; Disease; Disease Progression; Elements; Environment; Exhibits; Frequencies; Future; Goals; Health; Human; Hypertrophic Cicatrix; Implant; In Situ; Investigation; Liver; Liver Fibrosis; Magnetic Resonance Imaging; Magnetism; Measurement; Measures; Mechanics; Membrane; Methods; Modeling; Monitor; Mus; Onset of illness; Organ; Output; Parameter Estimation; Pathology; Pattern; Physiological; Process; Property; Reconstructive Surgical Procedures; Research; Residual state; Skin; Stress; Structure; Technology; Testing; Thinness; Tissue Expansion; Tissue Sample; Tissues; Validation; Vision; Work; biocompatible polymer; data-driven model; imaging modality; implantable device; improved; in vivo; kinematics; machine learning model; magnetic field; mechanical behavior; mechanical properties; new technology; non-invasive imaging; novel; particle; response; simulation; soft tissue; technology development; technology validation; tissue phantom; tissue stress; tool; ultrasound

Project Summary Soft connective tissues have remarkable mechanical functions, operating in the large deformation regime, showing highly nonlinear stress-strain response, and being physiologically under residual stress. Dysregulation of the tissue homeostatic state is associated with pathology, such as hypertrophic scar contracture and liver fibrosis. Progress in imaging modalities has opened a window into tissue kinematics in vivo in health and disease, for example high frequency ultrasound. Yet, measurement of mechanical properties in vivo remains out of reach. The state of the art in biomedical research remains ex vivo mechanical tests, which hinders progress in basic biomedical research towards understanding how tissues adapt mechanically in health and disease. Thus, for accurate measurement of the physiological mechanical environment of soft tissues, to better understand biomechanics of disease onset and progression, and eventually to improve diagnostics and treatment based on the evolving mechanics of soft tissue, new tools to measure mechanical properties in vivo are urgently needed. The objective of this proposal is to develop a novel remotely actuated deformable membrane to perform mechanical tests of soft tissue in vivo. We will develop a remotely actuated membrane capable of locally applying controlled stress fields to underlying tissue and measuring the ensuing deformation with high frequency ultrasound (Aim 1); develop a data-driven model of an active membrane adhered to a soft deformable substrate to enable parameter estimation from complex stress-strain data (Aim 2); and validate the technology on tissue phantoms, and murine skin and liver tissues ex vivo and in vivo (Aim 3). The work proposed here will result in a new technology to do in vivo mechanical tests of soft tissue, enabling progress of basic research in biomedical engineering. Development of this technology will open new possibilities to monitoring tissue mechanics in animal models of disease, expanding the current paradigm of kinematic tracking only. Our future work will continue in the direction of our long-term goal, towards development of implantable devices based on the same core technology proposed here.

项目概要 软结缔组织具有显着的机械功能，在大变形状态下运行， 显示出高度非线性的应力-应变响应，并且在生理上处于残余应力下。失调 组织稳态的变化与病理有关，例如肥厚性疤痕挛缩和肝脏 纤维化。成像方式的进步为了解健康和疾病中的体内组织运动学打开了一扇窗， 例如高频超声波。然而，体内机械性能的测量仍然遥不可及。 生物医学研究的最先进水平仍然是离体机械测试，这阻碍了基础研究的进展 生物医学研究旨在了解组织如何在健康和疾病中机械适应。因此，对于 准确测量软组织的生理力学环境，更好地了解 疾病发生和进展的生物力学，并最终改善诊断和治疗 随着软组织力学的不断发展，迫切需要测量体内力学性能的新工具。 该提案的目的是开发一种新型远程驱动变形膜来执行 体内软组织的力学测试。我们将开发一种能够局部应用的远程驱动膜 控制下方组织的应力场并高频测量随之而来的变形 超声波（目标 1）；开发粘附到软可变形基底的活性膜的数据驱动模型 能够根据复杂的应力-应变数据进行参数估计（目标 2）；并在组织上验证该技术 体模、小鼠皮肤和肝组织离体和体内（目标 3）。这里提出的工作将导致 进行软组织体内力学测试的新技术，促进生物医学基础研究的进展 工程。这项技术的发展将为监测动物组织力学开辟新的可能性 疾病模型，仅扩展了当前的运动学跟踪范式。我们未来的工作将继续在 我们的长期目标方向是开发基于相同核心的植入设备 这里提出的技术。