Microbiome Research Core

微生物组研究核心

• 批准号: 10659067
• 负责人: Brandon Briggs
• 金额: $ 18.63万
• 依托单位: UNIVERSITY OF ALASKA FAIRBANKS
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-16 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10659067
• 关键词: Active Learning; Alaska; Algorithms; American; Awareness; Basic Science; Bioinformatics; Biological; Bioreactors; Cells; Centers of Research Excellence; Clinical; Collaborations; Computer software; Computers; Consultations; Cytolysis; Data Analyses; Dedications; Dimensions; Discipline; Ecology; Elements; Ensure; Equipment; Event; Experimental Designs; Faculty; General Population; Genome; Goals; Health; Hibernation; Incubators; Infrastructure; Laboratory culture; Link; Metabolic; Metabolic Pathway; Metabolism; Microbe; Microbiology; Molecular; Molecular Analysis; Nutrient; Organism; Phase; Play; Procedures; Process; Research; Research Personnel; Research Project Grants; Research Support; Resources; Role; Services; Societies; Students; Symbiosis; System; Taxonomy; Technology; Training; Translational Research; Universities; bioinformatics tool; computerized data processing; host microbiome; microbial; microbial community; microbiome; microbiome research; nanopore; novel; outreach; programs; reconstruction; sample collection; symposium; tool

Project Summary - Microbiome Core Characterization of microbial communities and isolates is a key element necessary to achieve the scientific objectives of the proposed Center in Transformative Research in Metabolism. The Microbiome Core will provide a unique service dedicated to microbiome research and training. The goal of the Core is to support a comprehensive pipeline for characterization of microbial communities and isolates, which when characterized will generate testable hypotheses explaining host-microbiome symbiosis. We will achieve this goal by accomplishing the following Specific Aims (SA). SA1. Provide and maintain state-of-the-art technologies and procedures for a high throughput pipeline that characterizes microbial communities and isolates. SA2. Provide expertise in microbiome research, from experimental design and sample collection to data analysis and bioinformatics. SA3. Support and grow microbiome research and dissemination through expert consultation, and by engaging in outreach events with COBRE investigators and the general public. The novelty of this proposed core is to combine multiple modules that researchers can plug into at any point along the pipeline or access the full pipeline to characterize either isolates or microbial communities. The Microbiome Core is an essential component of the Research Projects supported by the Phase 1 center that focus on basic research into microbe-host symbiosis in metabolism. The Core provides the expertise, equipment, and software required for organismal, molecular, and bioinformatic analysis. Key equipment maintained by the Core includes a microbial bioreactor that allows users to set up experimental conditions similar to host guts and perturb the system to identify key microbial processes. An Illumina MiSeq and Nanopore MinION will provide the sequencing power to analyze the molecular underpinnings. In collaboration with the INBRE Bioinformatics Core, the Microbiome Core will provide expertise on bioinformatic algorithms needed for microbiome research.

项目摘要 - 微生物组核心 微生物群落和分离株的表征是实现科学评价所必需的关键要素 拟议的代谢变革研究中心的目标。微生物组核心将 提供致力于微生物组研究和培训的独特服务。核心的目标是支持 用于表征微生物群落和分离株的综合管道，当表征时 将产生解释宿主-微生物组共生的可检验假设。我们将通过以下方式实现这一目标 实现以下具体目标 (SA)。 SA1。提供并维护最先进的技术 用于表征微生物群落和分离物的高通量管道的程序。 SA2。提供 微生物组研究的专业知识，从实验设计和样本收集到数据分析和 生物信息学。 SA3。通过专家咨询支持和发展微生物组研究和传播， 并与 COBRE 调查人员和公众一起开展外展活动。这个的新颖之处 提议的核心是将多个模块组合起来，研究人员可以将其插入管道中的任何点或 访问完整的管道来表征分离株或微生物群落。微生物组核心是 一期中心支持的以基础研究为主的研究项目的重要组成部分 代谢过程中微生物与宿主的共生。核心提供所需的专业知识、设备和软件 用于有机体、分子和生物信息分析。核心维护的关键设备包括 微生物生物反应器，允许用户设置类似于宿主肠道的实验条件并扰乱 系统识别关键微生物过程。 Illumina MiSeq 和 Nanopore MinION 将提供 测序能力来分析分子基础。与 INBRE 生物信息学合作 微生物组核心将提供微生物组研究所需的生物信息算法专业知识。