Genetically Defined Herpes/Retrovirus SPF Macaques

基因定义的疱疹/逆转录病毒 SPF 猕猴

• 批准号: 7887502
• 负责人: DALLAS Melvin HYDE
• 金额: $ 47.13万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-20 至 2012-08-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7887502
• 关键词: Genetically; Defined; Herpes; Retrovirus; SPF

The goal of this proposal is to expand the production of genetically characterized rhesus macaques of Chinese-ancestry that are specific pathogen-free (SPF) for 7 persistent viruses, including simian immunodeficiency virus (SIV), simian type D retrovirus (SRV), simian T-lymphotropic virus (STLV), Cercopithecine herpesvirus 1 (B virus), simian foamy virus (SFV), rhesus cytomegalovirus (RhCMV) and rhesus rhadinovirus (RRV). This proposed expansion will be geared to the production of SPF animals for use in AIDS-related research, and to establishing a long-term SPF breeding colony of Chinese-origin rhesus macaques with known pedigrees and well-defined MHC genotypes. To meet these objectives, we will continue to derive SPF offspring from conventional, non-SPF Chinese rhesus breeding stock through nursery.-rearing of neonates. In addition, we will expand the existing Chinese-origin SPF colony through natural breeding and mother-rearing in social groups. An expanded program of MHC typing that includes additional microsatellite loci, class I alleles, and class II genes will be used to characterize offspring and their parents. This expanded MHC typing will allow the identification and definition of immune response genotypes, and will allow for selected breeding to produce animals with specific genetic profiles to respond to research needs. We also propose to collect longitudinal data on age-related changes in phenotype and function of lymphocyte subsets in cohorts of nursery-reared and mother-reared SPF rhesus macaques and in cohorts of SPF infants and non-SPF infants with differential exposure to infectious disease agents. Age- specific reference ranges, developed from normal, healthy SPF-rhesus macaques, are essential to more fully understand and utilize the macaque model system, and to provide comparative data allowing for more accurate interpretation of lymphocyte subset phenotype and functional alterations observed in AIDS and other infectious and immunologic diseases. Information on the genetics of immunity in SPF macaques will also impact the use of this valuable nonhuman primate for research on transplantation and stem cell/regenerative medicine. The scope of work proposed in this application will produce well characterized nonhuman primate animal resources for use in NIH-funded AIDS-related research. Genetic and immunologic data obtained from this project will better define and characterize the rhesus macaque model system in support of research on AIDS and other infectious diseases.

该提案的目的是扩大具有遗传特征的恒河猕猴的产生 中文疗法是7种持续病毒的特定无病原体（SPF），包括猿猴 免疫缺陷病毒（SIV），D型D型逆转录病毒（SRV），Simian T-淋巴机病毒（STLV），STLV）， Cercopithecine疱疹病毒1（B病毒），Simian Foamy病毒（SFV），恒河猴（RHCMV）和 鼠尾草病毒（RRV）。该提议的扩展将适合生产SPF动物供使用 在与艾滋病相关的研究中，并建立了中国王朝的长期SPF繁殖菌落 具有已知的血统和明确定义的MHC基因型的猕猴。为了实现这些目标，我们将 继续从传统的非SPF中国恒河猴繁殖库存中得出SPF后代 苗圃。新生儿的抚养。此外，我们将通过现有的中文SPF殖民地扩展 社会群体中的自然育种和母亲。扩展的MHC键入程序，其中包括 其他微卫星基因座，I级等位基因和II类基因将用于表征后代及其它们的表征 父母。这种扩展的MHC键入将允许识别和定义免疫反应 基因型，并将允许选定的育种产生具有特定遗传特征的动物以做出反应 用于研究需求。我们还建议收集有关表型与年龄相关的变化的纵向数据和 淋巴细胞亚群在苗圃和母亲饲养的SPF恒河猕猴中的功能 在SPF婴儿和非SPF婴儿中，暴露于传染病药物的情况下。年龄- 由正常的，健康的SPF逆转猕猴开发的特定参考范围对于更全面至关重要 了解和利用猕猴模型系统，并提供比较数据，以允许更多 在艾滋病和 其他传染病和免疫疾病。 SPF猕猴中免疫遗传学的信息将 还影响这种有价值的非人类灵长类动物在移植和STEM上的研究 细胞/再生医学。 本应用中提出的工作范围将产生良好的非人类灵长类动物 用于NIH资助的艾滋病相关研究的资源。从此获得的遗传和免疫数据 项目将更好地定义和描述恒河猕猴模型系统，以支持对艾滋病的研究 和其他传染病。