Animal Model and Genotyping Core Support Facility

动物模型和基因分型核心支持设施

• 批准号: 7733445
• 负责人: GLENN T. MERLINO
• 金额: $ 137.85万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7733445
• 关键词: Amendment; Animal Care and Use Committees; Animal Genetics; Animal Model; Animals; Area; B-Cell Lymphomas; Breeding; Cancer Biology; Communities; Computer software; DNA; Databases; Development; Equipment; Foundations; Genes; Genetic; Genomics; Genotype; Goals; Laboratories; Malignant Neoplasms; Malignant neoplasm of prostate; Mining; Monitor; Multiple Myeloma; Mus; NCI Center for Cancer Research; National Cancer Institute; Neoplasm Metastasis; Numbers; Pathway Analysis; Pharmaceutical Preparations; Plasma Cell Neoplasm; Plasmacytoma; Polymerase Chain Reaction; Preclinical Testing; Preparation; Research; Research Personnel; Research Support; Sequence Analysis; Sequence Homology; Single Nucleotide Polymorphism; Skin Carcinogenesis; Techniques; Writing; anticancer research; day; design; gel electrophoresis; malignant breast neoplasm; melanoma; mouse model; new technology; programs; research study; tool; tumor; tumor progression; tumorigenesis; vector

The mouse model animal and genotyping core provides support to investigators in the Laboratory of Cancer Biology and Genetics in several areas, all relating to mouse models of B cell lymphoma, myeloma, plasma cell tumorigenesis, skin carcinogenesis, melanoma, breast cancer and prostate cancer, all of which have been developed by various investigators in the group. In the animal core, we maintain the genetic integrity and oversee all of the breeding strategies required to maintain the many different lines of mice. The core staff carries out all genetic monitoring/genotyping. We routinely perform DNA extraction, genotyping of all mouse lines by PCR and gel electrophoresis. We actively participate in the planning, design, execution and analysis of tumor induction experiments and in setting up and executing preclinical testing of various drugs for their anti-tumor activity. We write many of the original Animal Study Proposals and associated amendments, and assure that they are kept up to date and in compliance with the NCI Animal Care and Use Committee. We actively participate in preparation for all inspections by the ACUC and AALAC. The animal core is responsible for coordinating the shipment of mouse lines as well as tumor lines to investigators all over the world, thus providing useful research tools to others in the cancer research community. The genomics section provides assistance to investigators within the laboratory, and in the Center for Cancer Research when appropriate, with respect to a wide variety of genomic applications including, but not limited to database mining, marker development, single nucleotide polymorphism (SNP) identification and analysis, sequencing and sequence homology searches. Programs currently supported include MacVector, DS Gene, Vector NTI, LaserGene, Sequencher, Partek, and Ingenuity Pathway Analysis. The combination of animal and genomic expertise in the core assists our investigators in their mechanistic studies of cancer initiation, cancer progression and cancer metastasis.

小鼠模型动物和基因分型核心为癌症生物学和遗传学实验室的研究者提供了支持，这些区域都与B细胞淋巴瘤，骨髓瘤，浆细胞肿瘤发生，皮肤致癌，黑色素癌，乳腺癌和前列腺癌的小鼠模型有关，所有这些都由该组的研究者开发。在动物核心中，我们保持遗传完整性，并监督维持许多不同小鼠所需的所有育种策略。核心人员进行所有遗传监测/基因分型。我们通常通过PCR和凝胶电泳对所有小鼠系进行DNA提取，基因分型。我们积极参与肿瘤诱导实验的计划，设计，执行和分析，并为各种药物进行抗肿瘤活性的临床前测试和执行临床前测试。我们撰写了许多原始动物研究建议和相关的修正案，并确保它们保持最新状态并符合NCI动物护理和使用委员会。我们积极参与ACUC和AALAC的所有检查准备。动物核心负责协调小鼠管路的运输以及向全世界的研究人员的肿瘤线的运输，从而为癌症研究界的其他人提供有用的研究工具。 “基因组学”部分在适当的情况下为实验室内的研究人员和癌症研究中心提供了帮助，这些基因组应用在各种基因组应用方面，包括但不限于数据库挖掘，标记开发，单核苷酸多态性（SNP）识别和分析，分析，测序和序列同源性搜索。 当前支持的程序包括MacVector，DS基因，Vector NTI，Lasergene，Sequencher，Partek和Ingenuity途径分析。动物和基因组专业知识在核心中的结合有助于我们的研究人员对癌症启动，癌症进展和癌症转移的机械研究。