Neutralization Mechanisms Of Anti-HIV-1 Monoclonal Antibodies

抗 HIV-1 单克隆抗体的中和机制

• 批准号: 7732745
• 负责人: Peter D Kwong
• 金额: $ 47.83万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7732745
• 关键词: Antibodies; Antibody Binding Sites; Binding; Binding Sites; Epitopes; HIV-1; Immune system; Infection; Lead; Membrane; Monoclonal Antibodies; Site; Vaccines; Viral; X-Ray Crystallography; neutralizing antibody; pathogen

A lack of neutralizing antibodies distinguishes HIV-1 from most other viral pathogens and is responsible to a large degree for the ability of HIV-1 to maintain a persistent infection. A few monoclonal antibodies have been isolated that are broadly neutralizing. We have used X-ray crystallography to investigate the epitopes of broadly neutralizing antibodies and their mechanisms of neutralization. In particular, we have examined CD4-induced (CD4i) antibodies that recognize the coreceptor-binding site, membrane-proximal-binding antibodies like 2F5, and CD4-binding-site antibodies like b12. The b12 analysis lead to the identification of a site of vulnerability to neutralizing antibody on HIV-1, which we are now pursuing as a prime vaccine target.

缺乏中和抗体将HIV-1与大多数其他病毒病原体区分开，并且在很大程度上负责HIV-1维持持续感染的能力。已经分离出一些大致中和的单克隆抗体。我们已经使用X射线晶体学来研究广泛中和抗体及其中和的机制的表位。 特别是，我们检查了CD4诱导的（CD4I）抗体，这些抗体识别良好的结合位点，膜 - 透明质结合抗体（如2F5）以及B12（如B12）等CD4粘合位点抗体。 B12分析导致鉴定了在HIV-1上中和抗体的脆弱性位点，我们现在正在将其作为主要疫苗靶标。

项目成果

The 447-52D antibody: hitting HIV-1 where its armor is thickest.

447-52D 抗体：攻击 HIV-1 装甲最厚的地方。

• DOI: 10.1016/j.str.2004.01.018
• 发表时间: 2004
• 期刊: Structure (London, England : 1993)
• 作者: Kwong,PeterD

Antigenicity and Immunogenicity in HIV-1 Antibody-Based Vaccine Design.

• DOI: 10.4172/2155-6113
• 发表时间: 2012
• 期刊: Journal of AIDS & clinical research
• 作者: Kong L;Sattentau QJ
• 通讯作者: Sattentau QJ