CAR BACILLUS INFECTION

汽车杆菌感染

• 批准号: 3421520
• 负责人: TRENTON R SCHOEB
• 金额: $ 11.29万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-30 至 1993-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3421520
• 关键词: Bacillus; Mycoplasma; antibacterial antibody; bacterial antigens; communicable diseases; enzyme linked immunosorbent assay; epizootiology; genetic strain; genotype; hybridomas; laboratory mouse; laboratory rat; monoclonal antibody; parainfluenza virus type 1; polymerase chain reaction; respiratory disorder diagnosis; respiratory epithelium; respiratory infections; tissue /cell culture; veterinary medicine

Cilia-associated respiratory (CAR) bacillus is a recently recognized respiratory pathogen of rats and mice. The pathogenesis of CAR bacillus disease has not been investigated thoroughly, and the prevalence of the organism is not known. However, identification of ten colonies with overt CAR bacillus disease by our laboratory in addition to reported cases suggests that infection could be common. Similarities between CAR bacillus disease and murine respiratory mycoplasmosis (MRM), such as parasitism of the surface of ciliated respiratory epithelial cells and the type, distribution, and duration of lesions, suggest that additional similarities probably exist. These include the existence of inapparent infections that may be difficult to detect, and contribution of ancillary factors such as concurrent viral infections to disease expression. If inapparent CAR bacillus infections occur, they could develop into severe respiratory disease under stresses associated with experimentation or concurrent infections acquired in user facilities, adversely affecting the use of rodents in biomedical research. Therefore, our overall objective is to assess the importance of CAR bacillus as a rodent pathogen, especially as a complicating factor in research with rats and mice. Specifically, we will: (i) determine the effects of host genotype, concurrent infection with other respiratory pathogens, exposure to gaseous ammonia, and CAR bacillus strain on expression of CAR bacillus disease in rats and mice; (ii) develop competition and mu-capture ELlSAs of documented sensitivity and specificity that are suitable for large-scale screening of laboratory animals for antibodies to CAR bacillus; and (iii) develop a method, based on amplification of CAR bacillus nucleic acid sequences by the polymerase chain reaction, for detecting CAR bacillus in either fixed, embedded tissues or in fresh tissue samples.

纤毛相关呼吸道（CAR）杆菌是最近公认的 大鼠和小鼠的呼吸道病原体。 汽车芽孢杆菌的发病机理 疾病尚未得到彻底研究，并且患病率 有机体是不知道的。 但是，鉴定了十个殖民地 我们的实验室除了报道 病例表明感染可能很常见。 汽车之间的相似之处 芽孢杆菌疾病和鼠呼吸道癌（MRM），例如 纤毛呼吸性上皮细胞表面的寄生虫和 病变的类型，分布和持续时间表明额外 相似之处可能存在。 这些包括不存在的存在 可能难以检测的感染和辅助的贡献 诸如疾病表达的病毒感染等因素。 如果 发生不适中的汽车芽孢杆菌感染，它们可能发育为严重 与实验或 在用户设施中获得的并发感染，对 在生物医学研究中使用啮齿动物。 因此，我们的整体 目的是评估汽车芽孢杆菌作为啮齿动物的重要性 病原体，特别是作为大鼠研究的复杂因素和 老鼠。 具体而言，我们将：（i）确定宿主基因型的影响， 同时感染了其他呼吸道病原体，暴露于 气态氨和汽车芽孢杆菌的表达表达 大鼠和小鼠的疾病； （ii）发展竞争和MU捕捉Ellsas 适合的敏感性和特异性 大规模筛查实验动物的汽车抗体 芽孢杆菌； （iii）基于汽车的扩增开发一种方法 通过聚合酶链反应的核酸杆菌序列，用于 检测固定，嵌入组织或新鲜的固定组织中的汽车芽孢杆菌 组织样品。