T CELL RECEPTOR SIGNALING DURING DEVELOPMENT

发育过程中的 T 细胞受体信号转导

• 批准号: 2429430
• 负责人: LISA M SPAIN
• 金额: $ 14.06万
• 依托单位: WISTAR INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-06-01 至 1999-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2429430
• 关键词: Retroviridae; T cell receptor; T lymphocyte; antigen presentation; biological signal transduction; cell differentiation; gene mutation; genetically modified animals; hybridomas; laboratory mouse; leukocyte activation /transformation; organ culture; site directed mutagenesis; thymus; tissue mosaicism

DESCRIPTION (Adapted from the Investigator's abstract): The goal of this research is to understand how the structure of the T-cell receptor determines its range of signaling capabilities. The investigator has chosen a genetic approach to determine which structures of the T-cell receptor (TCR) beta chain are important for signaling in T-cell development and in antigen recognition. The investigator will mutate specific domains and residues of the TCR beta chain and determine the effects of these mutations on T-cell development in thymic organ culture using retroviral vectors, and in transgenic/chimeric mice. Effects of TCR beta chain mutations on T-cell signaling will be measured in T-cell hybridomas and clones recognizing altered peptide ligands. Mutations will be introduced into the transmembrane domain, the variable and constant domain interface, and the constant domain loop.

描述（根据调查员的摘要改编）：目的 研究是了解T细胞受体的结构如何 确定其信号传导功能范围。 调查员选择了 确定T细胞受体哪些结构的遗传方法 （TCR）Beta链对于T细胞发育和中的信号传导很重要 抗原识别。 研究人员将突变特定领域，并 TCRβ链的残留物并确定这些突变的影响 关于使用逆转录病毒载体在胸腺培养中的T细胞发育，以及 在转基因/嵌合小鼠中。 TCRβ链突变对T细胞的影响 信号将在识别的T细胞杂交和克隆中测量 肽配体改变。 突变将被引入 跨膜域，变量和常数域接口，以及 恒定域循环。