GENETIC ANALYSIS OF QUINONE BINDING IN MEMPRANE PROTEINS

膜蛋白中醌结合的遗传分析

• 批准号: 5211693
• 负责人: EDWARD J BYLINA
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5211693
• 关键词: Rhodopseudomonas; chemical binding; electron transport; gel electrophoresis; membrane proteins; photosystem; protein engineering; protein purification; protein sequence; protein structure function; quinones

Quinones are ubiquitous in nature and play an important role in electron transfer and energy transduction. Protein interactions modify the properties of these quinones so that the same quinone species can perform many functions in the cell. The bacterial photosynthetic reaction center is an excellent model system to study these quinone-protein interactions, since it contains two well characterized, chemically distinct quinone binding sites whose structures are known to atomic resolution. We are currently developing a genetic system in Rhodopseudomonas viridis to study these interactions. Protein engineering of the photosynthetic reaction center will be utilized to study quinone binding. An understanding of the molecular basis of quinone-protein interactions and how these interactions modify the chemical properties of quinones in the reaction center can be applied to other electron transfer and energy transducing systems, including those of mitochondria. This research project will promote biomedical research in the following ways: (1) Membrane-bound protein complexes are involved in many important biological processes, including respiration, transport processes, transformation, sensory transduction, receptor binding, and signal transduction. The reaction center is the only membrane protein whose structure is known to atomic resolution. Insights into the correlation of structure and function in this system can be applied to these other systems. (2) The study of the molecular basis of quinone function in reaction centers will provide the student with an understanding of the mechanisms of electron transfer in biological systems and substrate binding in enzymes. (3) The genetic engineering techniques and principles that will be used by the student in this proposal can be applied to a wide variety of other projects of biomedical relevance.

醌在自然界中普遍存在，在电子中发挥着重要作用 转移和能量转换。 蛋白质相互作用改变 这些醌的性质，以便相同的醌种类可以发挥作用 细胞中的许多功能。 细菌光合作用反应中心 是研究这些醌-蛋白质相互作用的优秀模型系统， 因为它含有两种性质明确、化学性质不同的醌 其结构通过原子分辨率已知的结合位点。 我们是 目前正在开发绿色红假单胞菌的遗传系统 研究这些相互作用。 光合作用的蛋白质工程 反应中心将用于研究醌结合。 一个 了解醌-蛋白质相互作用的分子基础和 这些相互作用如何改变醌的化学性质 反应中心可应用于其他电子传递和能量 转导系统，包括线粒体的转导系统。 该研究项目将推动以下生物医学研究 途径：（1）膜结合蛋白复合物参与许多 重要的生物过程，包括呼吸、运输 过程、转化、感觉转导、受体结合，以及 信号转导。 反应中心是唯一的膜蛋白 其结构以原子分辨率已知。 洞察 该系统结构和功能的相关性可应用于 这些其他系统。 (2)醌的分子基础研究 反应中心的功能将为学生提供 了解生物中电子转移的机制 系统和酶中的底物结合。 (3)基因工程 学生在此过程中将使用的技术和原则 该提案可应用于生物医学的各种其他项目 关联。