PHOTOPHYSICS AND PHOTODYNAMIC EFFICIENCY OF HEMATOPORPHYRINS

血卟啉的光物理学和光动力效率

• 批准号: 5211544
• 负责人: JOHN W OWENS
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5211544
• 关键词: breast neoplasms; chemical structure function; clone cells; cytotoxicity; high performance liquid chromatography; laboratory mouse; neoplasm /cancer photoradiation therapy; nonhuman therapy evaluation; photosensitizing agents; porphyrin structure; porphyrins; quantum chemistry; tissue /cell culture

This projects seeks to improve the current state of photosensitization used in the detection and photodynamic therapy of neoplastic disease. Since the value of Hematoporphyrin Derative and Photofrin are well documented, this project will examine the electronic and fluorescent effects of three of the known components of HpD and Photofrin-II: protoporphyrin, hematoporphyrin, and dihematoporphyrin ether/ester. These three prophyrins will be examined by ultraviolet/visible and excitation/emission fluorescence spectrophotometry in the metal free and metallated Zinc forms. The metal free and Zinc metallated porphyrins will be examined in PBS (phosphate buffered saline; ph-7.2) free of cells and in PBS solutions containing PTK2 cells (rat kangaroo epithelial kidney cells). The respective porphyrin solutions containing the PTK2 cells will also be subjected to photoirradiation form a dye laser2 to examine the phototoxicity of the metal-free and metallated Zinc porphyrins. The specific aims of this project are to test Zinc substituted porphyrin complexes against known components of HpD and Photofrin-II. The effects to be examined include the following: 1). identification and assignment of electronic transitions. 2). a comparison of the fluorescence lifetime and quantum yields, 3). measurements and comparisons of the photokill ratios. The comparison of HpD and Photofrin-II components against their metallated Zinc analogues is important because it is well known that metalloporphyrins are better able to fluoresce and phosphoresce than their demetallated counterparts. If it can be shown that Zinc substituted protoporphyrin, for example, exhibits a superior fluorescence than does protoporphyrin in the demetallated form, this complex could be used as a localizer for cancer cells. If any one of these Zinc-porphyrins exhibited superior sensitization of cells, they could be used as photosentizers, either as precursors for or in conjunction with HpD.

该项目旨在改善当前光敏的状态 用于肿瘤疾病的检测和光动力疗法。 由于血源性肾蛋白衍生物和光蛋白的值很好 记录的，该项目将检查电子和荧光 HPD和Photofrin-II的三个已知成分的影响： 原磷脂，造血脊髓磷脂和二脑甲磷脂醚/酯。这些 三个先知蛋白将通过紫外线/可见和 兴奋/发射荧光分光光度计在无金属和 金属锌形式。金属和锌金属的卟啉将 在PBS（磷酸盐缓冲盐水; pH-7.2）中进行检查，没有细胞和 在包含PTK2细胞的PBS溶液中（大鼠袋鼠上皮肾脏 细胞）。包含PTK2细胞的各自的卟啉溶液将 还要受到光辐射形式的染料激光2检查 无金属和金属锌卟啉的光毒性。 该项目的具体目的是测试锌取代的卟啉 针对HPD和Photofrin-II的已知成分的复合物。效果 待检查包括以下内容：1）。标识和分配 电子过渡。 2）。荧光寿命的比较 和量子产量，3）。光数字的测量和比较 比率。 HPD和Photofrin-II成分与它们的比较 金属锌类似物很重要，因为众所周知 金属核蛋白比荧光和磷酸盐更好 他们被淘汰的同行。如果可以证明锌取代 例如，原源性的荧光比 原化形式的原源性磷脂，该复合物可以用作 癌细胞的本地化。如果这些锌 - 卟啉中有任何一个 表现出对细胞的较高敏化，可以用作 摄影剂是作为HPD的前体或结合使用的前体。