Optical Tools to Study Neuropeptide Signaling
研究神经肽信号转导的光学工具
基本信息
- 批准号:9135392
- 负责人:
- 金额:$ 22.68万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2015
- 资助国家:美国
- 起止时间:2015-09-01 至 2018-08-31
- 项目状态:已结题
- 来源:
- 关键词:Absence of pain sensationAffinityAlcohol dependenceAminobutyric AcidsAnxietyAttentionBehaviorBindingBiosensorBlood - brain barrier anatomyBolus InfusionBrainCalciumCell physiologyCellsCharacteristicsCognitionDevelopmentDiffuseDisease modelDistantDrug AddictionDynorphinsElectrophysiology (science)End Point AssayEngineeringEnkephalinsG-Protein-Coupled ReceptorsGlutamatesGoalsHealthIn Situ HybridizationInjection of therapeutic agentKnowledgeLeadLeftLifeLightMental DepressionMethodologyMethodsMicrodialysisMolecularMotorNeuromodulatorNeuropeptidesNeurotransmittersOpioidOpticsOxygenPainParkinson DiseasePathway interactionsPeptidesPharmacologyPhysiologyPlayProcessProtein EngineeringProteinsReporterResolutionRoleSignal TransductionSiteSliceSynapsesSynaptic TransmissionSystemTertiary Protein StructureTimeTissuesVariantaddictionanimal imagingbasebehavioral pharmacologybrain tissuelight microscopylive cell imagingnervous system disorderneural circuitneurotransmissionoptogeneticspainful neuropathyrelating to nervous systemspatiotemporaltemporal measurementtooltransmission processvoltage
项目摘要
DESCRIPTION (provided by applicant): Neuropeptides are neuromodulators that regulate the physiology of cells, synapses, and neural circuits in the brain. For example, opioid neuropeptides are involved in pain and analgesia, and altered opioid neuropeptide levels have been observed in addiction, depression and anxiety, as well as a number of other neurological disorders including Parkinson's disease. Clearly, neuropeptides play a significant role in modulating behavior and cognition, but many aspects of neuropeptide signaling are not well understood. Unlike classical fast synaptic transmitters such as glutamate and GABA, neuropeptides can be released both at the synapse and outside the synapse. Neuropeptides can also diffuse significant distances from where they were released. At distant sites, neuropeptides can continue to function as signals at low concentrations by activating high affinity G-protein coupled receptors. Thus, neuropeptides engage in signaling over much broader spatial and temporal scales than synaptic transmission, and these spatiotemporal characteristics have made it difficult to precisely study how neuropeptide signals propagate throughout the brain. This experimental barrier has left important gaps in our knowledge of how the neural circuits that are responsible for behavior and cognition are modulated. Therefore, in order to overcome this critical barrier and enable neuropeptides to be directly studied in healthy and diseased brain tissue, it is the goal of this proposal is to develop new genetically-encoded optical tools to (1) record and (2) modulate neuropeptide signaling.
描述(由申请人提供):神经肽是调节大脑中细胞、突触和神经回路生理学的神经调节剂,例如,阿片类神经肽参与疼痛和镇痛,并且已在成瘾、抑郁和抑郁症中观察到阿片类神经肽水平。焦虑以及包括帕金森氏病在内的许多其他神经系统疾病显然,神经肽在调节行为和认知方面发挥着重要作用,但神经肽信号传导的许多方面尚不清楚。与谷氨酸和 GABA 等经典的快速突触递质不同,神经肽可以在突触内和突触外释放。因此,神经肽在比突触传递更广泛的空间和时间尺度上参与信号传导,并且这些时空特征使得很难精确研究神经肽信号如何在整个大脑中传播,这一实验障碍在我们对负责行为和认知的神经回路如何调节的知识中留下了重要的空白,因此,为了克服这一关键障碍并使神经肽能够直接传播。在健康和患病的脑组织中进行研究,该提案的目标是开发新的基因编码光学工具来(1)记录和(2)调节神经肽信号传导。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
pH- and Temperature-Dependent Peptide Binding to the Lactococcus lactis Oligopeptide-Binding Protein A Measured with a Fluorescence Anisotropy Assay.
使用荧光各向异性测定测量 pH 和温度依赖性肽与乳酸乳球菌寡肽结合蛋白 A 的结合。
- DOI:
- 发表时间:2019-02-28
- 期刊:
- 影响因子:4.1
- 作者:Norcross, Stevie;Sunderraj, Ashwin;Tantama, Mathew
- 通讯作者:Tantama, Mathew
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Mathew Tantama其他文献
Mathew Tantama的其他文献
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{{ truncateString('Mathew Tantama', 18)}}的其他基金
Optical Tools to Study Purinergic Signaling - Administrative Supplement
研究嘌呤能信号传导的光学工具 - 行政补充
- 批准号:
10591310 - 财政年份:2022
- 资助金额:
$ 22.68万 - 项目类别:
Optical Tools to Study Purinergic Signaling - Administrative Supplement
研究嘌呤能信号传导的光学工具 - 行政补充
- 批准号:
10580281 - 财政年份:2022
- 资助金额:
$ 22.68万 - 项目类别:
Optical Tools to Study Purinergic Signaling - Diversity Supplement Postbaccalaureate
研究嘌呤能信号传导的光学工具 - 本科后多样性补充
- 批准号:
10621984 - 财政年份:2022
- 资助金额:
$ 22.68万 - 项目类别:
Genetically-encoded optical sensors to study purinergic signaling
用于研究嘌呤能信号传导的基因编码光学传感器
- 批准号:
8995713 - 财政年份:2015
- 资助金额:
$ 22.68万 - 项目类别:
Compartmentation of Neuronal ATP and Metabolic Regulation of Excitability
神经元 ATP 的划分和兴奋性的代谢调节
- 批准号:
8056931 - 财政年份:2011
- 资助金额:
$ 22.68万 - 项目类别:
Compartmentation of Neuronal ATP and Metabolic Regulation of Excitability
神经元 ATP 的划分和兴奋性的代谢调节
- 批准号:
8424723 - 财政年份:2011
- 资助金额:
$ 22.68万 - 项目类别:
Compartmentation of Neuronal ATP and Metabolic Regulation of Excitability
神经元 ATP 的划分和兴奋性的代谢调节
- 批准号:
8442324 - 财政年份:2011
- 资助金额:
$ 22.68万 - 项目类别:
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