MOLECULAR CONTROL OF BLOOD VESSEL FORMATION

血管形成的分子控制

• 批准号: 2415457
• 负责人: Victoria L Bautch
• 金额: $ 6.61万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-05-14 至 1998-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2415457
• 关键词: angiogenesis; angiogenesis factor; cell adhesion molecules; cell cell interaction; developmental genetics; embryogenesis; embryonic stem cell; genetic library; genetic transcription; genetically modified animals; growth factor receptors; growth media; in situ hybridization; laboratory mouse; molecular cloning; polymerase chain reaction; receptor expression; tissue /cell culture; vascular endothelium

The proposed research seeks to develop in vitro models of blood molecules that may be involved in this process by i) determining how murine endothelial cells derived from transgenic mouse tumors model and interact with normal endothelial cells; ii) determining if cystic embryoid bodies that are differentiated in vitro from mouse embryonic stem cells mimic the mouse yolk sac in the expression pattern of various molecules, iii) determining the sensitivity of in vitro vascular development to exogenous factors, and iv) identifying novel genes that may be involved in vascular development.

拟议的研究旨在发展血液的体外模型 通过i）确定如何参与此过程的分子 来自转基因小鼠肿瘤模型的鼠内皮细胞和 与正常内皮细胞相互作用； ii）确定是否囊性 在体外与小鼠胚胎分化的胚胎体 干细胞模仿小鼠蛋黄囊以各种表达方式 分子，iii）确定体外血管的敏感性 发展到外源性因素，iv）识别新的基因 可能参与血管发育。

项目成果

In vitro differentiation of mouse ES cells: hematopoietic and vascular development.

小鼠 ES 细胞的体外分化：造血和血管发育。

• DOI: 10.1016/s0076-6879(03)65006-8
• 发表时间: 2003
• 期刊: Methods in enzymology
• 作者: Kearney,JosephB;Bautch,VictoriaL
• 通讯作者: Bautch,VictoriaL