Clemson Light Imaging Facility (CLIF)

克莱姆森光学成像设备 (CLIF)

基本信息

批准号：
8813282
负责人：
Terri Lane Foster Bruce
金额：
$ 19.18万
依托单位：
CLEMSON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8813282
关键词：
Address Biochemical Biological Sciences Cell Count Cell Separation Cell physiology Cells Centers of Research Excellence Charge Communities Conceptions Core Facility Cytometry Data Development Educational workshop Equipment Faculty Fees Floor Flow Cytometry Fluorescence-Activated Cell Sorting Fostering Funding Generations Goals Grant Guava Fruit Housing Image Imagery Imaging Techniques Institution Laboratories Light Light Microscope Location Microscope Microscopy Multiphoton Fluorescence Microscopy Organelles Preparation Research Research Infrastructure Research Personnel Research Project Grants Research Technics Resolution Running Sorting - Cell Movement South Carolina Specimen Students Targeted Research Technical Expertise Techniques Technology Time Training Training Support United States National Institutes of Health Universities Work base collaborative environment design equipment training fluorescence activated cell sorter device graduate student imaging system improved innovation instrumentation interest light microscopy meetings member pathogen polarized light research study tool university student user-friendly

项目摘要

Summary: The Clemson Light Imaging Facility (CLIF) will serve as the Imaging and Cell Sorting Core for the EPIC COBRE. The CLIF is a multi-user core facility located in the new Clemson University Life Sciences Facility. The core is under the direction of Dr. Terri Bruce, and houses a suite of Nikon microscopes, a Zeiss LSM 510 confocal microscope, a CytoViva Hyperspectral Imaging System, a Leica DM 750 P polarized light microscope, a Leica DM IL inverted contrasting microscope, a Leica M80 stereomicroscope, a Leica TCS SPE spectral confocal and a Leica SP8X multiphoton spectral confocal microscope. Additionally, the CLIF houses cell counting and sorting equipment, including a Guava EasyCyte HT and a BD Influx FACS cell sorter. The CLIF includes a multi-user specimen preparation laboratory, and a dedicated 20-seat classroom equipped with video conferencing and recording capabilities. The specific aims of the Imaging and Cell Sorting Core are 1) to enhance the cell sorting capabilities of the Clemson Light Imaging Facility (CLIF); 2) to promote and facilitate the research undertaken by the target PIs of this COBRE; and 3) to promote and facilitate research at Clemson University and the state of South Carolina. Aim 1 will be addressed by the addition of a BioRad S3 Cell Sorter (funds from CU Provost) and a dedicated cell sorting technician. Aims 2&3 will be addressed through providing assistance to target investigators with design and implementation of visualization and cell sorting experiments, providing advanced technique and equipment training, and providing organized workshops on multiphoton/confocal imaging and advanced cell sorting.

摘要：克莱姆森光学成像设施 (CLIF) 将作为成像和细胞分选核心史诗般的COBRE。 CLIF 是位于新克莱姆森大学生命科学中心的多用户核心设施设施。核心由 Terri Bruce 博士指导，配备一套尼康显微镜、一台蔡司显微镜 LSM 510 共焦显微镜、CytoViva 高光谱成像系统、Leica DM 750 P 偏振光显微镜、Leica DM IL 倒置对比显微镜、Leica M80 体视显微镜、Leica TCS SPE 光谱共焦显微镜和 Leica SP8X 多光子光谱共焦显微镜。此外，CLIF 还设有细胞计数和分选设备，包括 Guava EasyCyte HT 和 BD Influx FACS 细胞分选仪。这 CLIF 包括一个多用户标本制备实验室和一个配备 20 座位的专用教室视频会议和录音功能。成像和细胞分选核心的具体目标是 1) 增强细胞分选能力克莱姆森光学成像设备（CLIF）； 2) 促进和促进目标PI进行的研究这个 COBRE； 3) 促进和促进克莱姆森大学和南卡罗来纳州的研究。目标 1 将通过添加 BioRad S3 细胞分选仪（由 CU 教务长提供资金）和专用的细胞分选技术员。目标 2 和 3 将通过向目标调查人员提供协助来解决可视化和细胞分选实验的设计和实施，提供先进的技术和设备培训，并提供有组织的多光子/共焦成像和先进细胞研讨会排序。