Einstein Mount Sinai Diabetes Research Center

爱因斯坦西奈山糖尿病研究中心

• 批准号: 10618196
• 负责人: Irwin Jack Kurland
• 金额: $ 33.57万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10618196
• 关键词: Adenoviruses; Adipocytes; Anabolism; Animal Model; Animals; Biochemical; Biological; Biological Assay; Biological Sciences; Carbohydrates; Cells; Citric Acid Cycle; Clinical Investigator; Collection; Computer Analysis; Core Facility; Coupled; Data Analyses; Diabetes Mellitus; Diagnosis; Dissection; Experimental Designs; Feces; Gas Chromatography; Genus Hippocampus; Glucose; Glycerol; Glycolysis; Goals; Hepatic; Homeostasis; Human; Human Resources; In Vitro; Infrastructure; Infusion procedures; Islet Cell; Isotopes; Lipids; Liquid substance; Lymph; Measurement; Metabolic; Metabolic Pathway; Metabolism; Mission; Mitochondria; Molecular; Muscle; Osmosis; Oxygen Consumption; Pathway interactions; Peripheral; Phosphocreatine; Physiological; Physiology; Plasma; Preparation; Production; Protein Biosynthesis; Pump; Recycling; Research; Research Personnel; Resolution; Respiration; Rodent; Services; Students; Technology; Time; Tissue Sample; Tissues; Tracer; Translational Research; Update; Urine; Water; Work; analytical method; cost effective; dalton; design; experimental study; extracellular; glucose disposal; glucose production; human model; implantation; improved; in vivo; inorganic phosphate; instrument; ion mobility; islet; lipid biosynthesis; lipidomics; liquid chromatography mass spectrometry; metabolic rate; metabolomics; stable isotope; synergism; tissue culture; tissue/cell culture

The goal of the Stable Isotope and Metabolomics Core (SIMC) is to provide to Einstein-Mount Sinai Diabetes Research Center research investigators cost effective, efficient and robust platforms for the determination of metabolites/lipids designed to cover interlocking metabolite and lipid pathways, and help to improve our understanding of conditions which change fuel utilization and biosynthesis in cytosolic and mitochondrial metabolic pathways, i.e., glycolysis, pentose and tricarboxylic acid (TCA) cycles. To accomplish these goals in one facility, the SIMC has updated its technology infrastructure to include the latest Seahorse XFe24 and XFe96 Seahorse Flux Analyzers for assessment of glycolytic and mitochondrial energetics in real time, the latest Sciex 6500+ QTRAP with Selexion ion mobility for targeted LC/MS.MS metabolite and lipidomic analyses, and is due to replace its Orbitrap/LTQ high mass accuracy instrument with the latest Shimadzu LC-9030 quadrupole Time of Flight with Nexera UPLC for untargeted metabolomic and lipidomic analyses. These latest additions add to the high mass resolution Waters GC-TOF/MS and two unit Dalton resolution Agilent single quadrupole GC/MS machines to accomplish the following aims: 1. To determine metabolomic/lipidomic profiles for the diagnosis/characterization of physiological and pathophysiological states in cells, tissues and biofluids (plasma, urine, stool, etc.). 2. To perform in vivo stable isotope substrate assays to determine rates of protein synthesis, lipogenesis, peripheral glucose disposal, hepatic glucose recycling, glucose-glycerol cycling and Cori cycling, high energy phosphate (ATP, creatine phosphate) turnover, and in vitro stable isotope flux dissections of metabolic pathways. 3. To perform assessments of glycolysis (extracellular acidification rates, glycolytic ATP production rates) and mitochondrial oxygen consumption (mitochondrial respiration and mitochondrial ATP production rates) in tissue explants, primary isolated and tissue culture cells using Seahorse Biosciences Flux Analyzers. 4. To advise and instruct ES-DRC investigators and their students, fellows and technical staff in the design and interpretation of fluxomics, metabolomics/lipidomics and computational analyses with the aim of elucidating the molecular basis underlying glucose and lipid homeostasis in humans and animal models.

稳定的同位素和代谢组学核心（SIMC）的目的是为爱因斯坦安装提供 西奈糖尿病研究中心研究人员的成本效益，高效且健壮 用于测定代谢物/脂质的平台，旨在覆盖互锁代谢物 和脂质途径，并有助于提高我们对改变燃料的条件的理解 胞质和线粒体代谢途径的利用和生物合成，即糖酵解， 五羧酸（TCA）循环。为了在一个设施中实现这些目标， 已更新了技术基础设施，包括最新的Seahorse XFE24和XFE96 用于评估糖酵解和线粒体能量学评估的海马通量分析仪， 最新的SCIEX 6500+ QTRAP带有针对目标LC/MS.MS代谢物的SELEXION离子移动性和 脂质组分析，并将用其Orbitrap/LTQ高质量精度使用 最新的Shimadzu LC-9030四极杆的飞行时间与Nexera UPLC无关 代谢组学和脂肪组分析。这些最新添加增加了高质量分辨率 水域GC-TOF/MS和两个单位Dalton分辨率Agilent单四极杆GC/MS机器 实现以下目标： 1。确定代谢组/脂肪组谱以诊断/表征 细胞，组织和生物流体中的生理和病理生理态（血浆，尿液，， 凳子等）。 2。执行体内稳定的同位素底物测定以确定蛋白质合成的速率， 脂肪生成，外周葡萄糖处置，肝葡萄糖回收，葡萄糖 - 甘油循环 和Cori Cycling，高能磷酸盐（ATP，肌酸磷酸盐）营业额和体外 代谢途径的稳定同位素通量解剖。 3。进行糖酵解的评估（细胞外酸化速率，糖酵解ATP 生产率）和线粒体氧的消耗（线粒体呼吸和 线粒体ATP生产率）在组织外植体中，主要分离和组织培养 使用Seahorse Biosciences通量分析仪的细胞。 4。为ES-DRC调查人员及其学生，研究员和技术人员提供建议和指导 在通量学，代谢组学/脂质组学和计算的设计和解释中 分析的目的是阐明分子基础葡萄糖和脂质 人类和动物模型中的稳态。