High Throughput Sciences

高通量科学

基本信息

批准号：
10617303
负责人：
Angela Nicole Koehler
金额：
$ 22.14万
依托单位：
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-06-17 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10617303
关键词：
Biocompatible Materials Bioinformatics Biological Biological Assay Budgets Cancer Center Cancer Center Support Grant Cell Line Cells ChIP-seq Clustered Regularly Interspaced Short Palindromic Repeats Collaborations Collection Consultations Core Facility Custom Data Data Analyses Data Set Development Drug Delivery Systems Education Educational workshop Enzyme-Linked Immunosorbent Assay Equipment Evaluation Funding Genetic Genomics Image Imaging technology Information Centers Institution Libraries Library Materials Liquid substance Methods Mission Mus Mycoplasma Preparation Research Research Personnel Resource Sharing Resources Robotics Role Sampling Science Series Services Source Stains System Systems Biology Technology Testing Toxicology Training Validation Zebrafish assay development design drug discovery exome gene discovery instrumentation interest light scattering machine learning algorithm member programs response screening small molecule small molecule libraries success technology platform tissue processing tool training opportunity

项目摘要

High Throughput Sciences Core: Project Summary/Abstract The Koch Institute High Throughput Sciences Core (HTS Core) provides automated technologies, expertise and training that allows Cancer Center Members to investigate the interplay between biomaterials, biomolecules, small molecules and cells in a high-throughput, multiplexed manner to enable drug and gene discovery, drug delivery, toxicology, and systems biology applications. The HTS Core possesses state-of-the-art robotic instrumentation, an array of biological tools, including validated cell lines and curated libraries, and an exceptional staff with expertise in assay development and adaptation, screening execution, and robotics workflows. HTS Core activities were established in 2011, with support from CCSG Developmental Funds and Institutional support. In the prior submission, HTS activities were embedded in the Koch Institute Genomics Core, reflecting their extensive interactions. During this current period, the HTS Core has greatly expanded its roles and impact for both Center Members and other Koch Institute Cores. Given this, and on the advice of our SAB, we are requesting funding for High Throughput Sciences as a stand-alone Core. In the current funding period, the HTS Core added a significant number of new capabilities and modified existing services, including: a new high throughput flow cytometer and tissue processing equipment; added >55,000 new compounds to small molecule libraries; automated workflows for ELISAs, high content imaging and HT sample preparation pipelines, including automated plate-based HT ChIP-Seq sample preparation (collaborating with the Integrated Genomics & Bioinformatics Core); and cell line management services, including mycoplasma testing and banking of mycoplasma-free cell lines. Center Member Core use has been strong: 66% of Center Members use the Core, account for 73% of Core service use, and include investigators from all three Research Programs. In the upcoming period, the HTS Core will continue to offer a wide range of state-of-the-art services to support Center Member research programs, and will evaluate emerging capabilities in the context of Center Member needs and interests. A number of new initiatives are planned, including: acquisition and evaluation of new equipment; upgrades to existing equipment; acquisition of new CRISPR and targeted small molecule libraries; establishment of small molecule screening in zebrafish; continued collaboration with other Koch Institute Core Facilities to develop technology and streamline workflows; deployment of a custom-built LIMS system to manage library materials and screening assay data; dataset sharing to support development of machine learning algorithms; and collaborating with other Koch Institute Cores to develop a monthly workshop series to support Center Members’ access to imaging technology platforms and data analysis. This Shared Resource is essential to the success of the Koch Institute mission and provides exceptional value to the CCSG. The requested CCSG budget for Year 49 is increased by 1.6% over the Core CCSG budget for the current period (Year 48).

高通量科学核心：项目摘要/摘要科赫研究所高通量科学核心（HTS Core）提供自动化技术、专业知识和允许癌症中心成员调查生物材料、生物分子、小分子之间的相互作用的培训以高通量、多重方式分析分子和细胞，以实现药物和基因发现、药物输送、 HTS Core 拥有最先进的机器人仪器、毒理学和系统生物学应用。一系列生物工具，包括经过验证的细胞系和精选的文库，以及具有以下专业知识的优秀员工分析开发和适应、筛选执行和机器人技术工作流程。成立于 2011 年，得到 CCSG 发展基金和机构的支持。根据提交的材料，HTS 活动被嵌入到科赫研究所基因组学核心中，反映了其广泛的在此期间，HTS 核心极大地扩展了其对两个中心的作用和影响。鉴于此，根据我们的 SAB 的建议，我们正在请求资金。高通量科学作为独立的核心。在当前资助期内，HTS Core 添加了大量新功能并修改了现有功能服务，包括：新增超过 55,000 台新型高通量流式细胞仪和组织处理设备；化合物到小分子库；ELISA、高内涵成像和 HT 样品的自动化工作流程制备流程，包括基于板的自动化 HT ChIP-Seq 样品制备（与综合基因组学和生物信息学核心）和细胞系管理服务，包括支原体检测和无支原体细胞系的银行库中心成员核心使用率很高：66% 的中心成员使用该系统。核心，占核心服务使用的 73%，包括来自所有三个研究计划的研究人员。在接下来的一段时间内，HTS Core 将继续提供广泛的最先进的服务来支持中心成员研究计划，并将根据中心成员需求评估新兴能力计划采取一些新举措，包括：新设备的采购和评估；升级现有设备；购买新的CRISPR和靶向小分子库；斑马鱼小分子筛选；继续与科赫研究所其他核心设施合作开发技术并简化工作流程；部署定制的 LIMS 系统来管理图书馆资料和筛选分析数据；数据集共享以支持机器学习算法的开发和协作；与其他科赫研究所核心人员一起开发每月研讨会系列，以支持中心成员获得该共享资源对于科赫的成功至关重要。制定使命并为 CCSG 提供非凡价值要求的第 49 年 CCSG 预算为：比本期（第 48 年）核心 CCSG 预算增加 1.6%。