Investigating the role of correlated neural activity in pair bonding using prairie voles

使用草原田鼠研究相关神经活动在配对中的作用

• 批准号: 10617698
• 负责人: Kathleen Murphy
• 金额: $ 4.02万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-05-16 至 2024-11-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10617698
• 关键词: Address; Animals; Automobile Driving; Behavior; Behavioral; Behavioral Paradigm; Calcium; Chiroptera; Complex; Control Groups; Couples; Data; Disease; Exhibits; Feedback; Fiber; Fluorescence; Frequencies; Friends; Health; Human; Impairment; Individual; Laboratories; Linear Models; Machine Learning; Mammals; Measures; Medial; Mediating; Mental disorders; Microtus; Modernization; Mus; Nature; Neurons; Neurosciences; Nose; Optics; Pair Bond; Partner in relationship; Pattern; Personal Satisfaction; Persons; Photometry; Posture; Prefrontal Cortex; Proxy; Research Personnel; Risk; Role; Side; Signal Transduction; Social Behavior; Social Environment; Social Interaction; Techniques; Testing; Therapeutic; Time; Touch sensation; Training; Work; affiliative behavior; autism spectrum disorder; experience; experimental study; fluorophore; neural; neural correlate; optogenetics; prairie vole; preference; sensor; sex; skills; social attachment; social deficits; social engagement; social relationships; supervised learning; tool; unsupervised learning

PROJECT SUMMARY Romantic bonds are a fundamental part of the human experience, positively contributing to our health and well-being by decreasing risk for physical and mental illness. A key feature of pair bonds - and social relationships more generally - is that they are highly reciprocal, evolving as a function of ongoing feedback from the other individual. Yet the interdependent nature of neural activity within pair bonding remains understudied, due in part to the dearth of laboratory-amenable mammals that form selective attachments with their mating partners. One promising metric for explaining aspects of interaction and its progression in dynamic social contexts is interbrain synchronization, or correlation, in which the neural dynamics of two individuals become more similar to each other as they interact. Interbrain correlation scales with relational variables; it is stronger in romantic partners than strangers during social interaction. I have found that interbrain correlation is also evident in prairie voles and that pair bonded voles show stronger correlations when interacting with their partner than with a stranger. In this proposal, I will leverage modern optical tools to detect and functionally manipulate interbrain correlation in prairie voles. In my first aim, I will perform within-animal comparisons to determine how interbrain correlation varies across discrete behaviors and as a function of pair bonding. I will use fiber-photometry to conduct simultaneous real-time calcium recordings in the medial prefrontal cortex of interacting animals, and use supervised and unsupervised machine learning to conduct thorough behavioral annotations of the interaction. Using a variety of analytical approaches, including generalized linear models, I will delineate the relationship between pair bonding, affiliative behavior and interbrain correlation. In my second aim, I will use synchronized real-time optogenetic stimulation in the mPFC to test whether induced interbrain correlation can facilitate pair bond formation. Together these results will characterize the occurrence of interbrain correlation during distinct behaviors and as a function of bond state and test the function of interbrain correlation in pair bond formation. Such work is instrumental for determining whether therapeutic approaches targeting interbrain correlation will be beneficial for treating disorders that impair social attachment like autism spectrum disorders.

项目摘要 浪漫纽带是人类经验的基本组成部分，为我们的健康做出了积极贡献 以及通过降低身体和精神疾病的风险来幸福。配对债券和社交的关键特征 关系更一般 - 它们是高度互惠的，随着正在进行的反馈的函数而演变 来自另一个人。然而，配对键内神经活动的相互依赖性质仍然存在 研究了，部分是由于缺乏实验室无哺乳动物，这些哺乳动物与 他们的交配伙伴。一种有希望的指标，用于解释相互作用的各个方面及其在动态方面的发展 社会背景是脑间同步或相关性，其中两个人的神经动力学 当互动时变得更加相似。脑间相关量表与关系变量；这是 在社交互动期间，浪漫伴侣比陌生人更强大。我发现脑之间的相关性是 在草原田鼠中也很明显，与它们的互动时，对田鼠的相互作用也更强 伴侣比和一个陌生人。在此建议中，我将利用现代光学工具来检测和功能 在草原田鼠中操纵脑间相关性。在我的第一个目标中，我将进行自动比较 确定脑间相关性如何在离散行为和成对键合的函数中变化。我会 使用光纤光学法在内侧前额叶皮层中同时进行实时钙记录 相互作用的动物，并使用受监督和无监督的机器学习进行彻底的行为 相互作用的注释。使用多种分析方法，包括通用线性模型，I 将描绘成对键，隶属行为和脑间相关性之间的关系。在我的第二个 目的，我将在MPFC中使用同步的实时光遗传刺激来测试是否诱导脑 相关可以促进成对键的形成。这些结果在一起将表征 在不同的行为和键状态的函数和脑的功能中，脑间相关性的脑部相关性 成对键形成中的相关性。此类工作对确定治疗方法是否有用 靶向脑之间的相关性将有益于治疗损害社会依恋等自闭症的疾病 频谱障碍。