Investigating the role of correlated neural activity in pair bonding using prairie voles

使用草原田鼠研究相关神经活动在配对中的作用

• 批准号: 10617698
• 负责人: Kathleen Murphy
• 金额: $ 4.02万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-05-16 至 2024-11-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10617698
• 关键词: Address; Animals; Automobile Driving; Behavior; Behavioral; Behavioral Paradigm; Calcium; Chiroptera; Complex; Control Groups; Couples; Data; Disease; Exhibits; Feedback; Fiber; Fluorescence; Frequencies; Friends; Health; Human; Impairment; Individual; Laboratories; Linear Models; Machine Learning; Mammals; Measures; Medial; Mediating; Mental disorders; Microtus; Modernization; Mus; Nature; Neurons; Neurosciences; Nose; Optics; Pair Bond; Partner in relationship; Pattern; Personal Satisfaction; Persons; Photometry; Posture; Prefrontal Cortex; Proxy; Research Personnel; Risk; Role; Side; Signal Transduction; Social Behavior; Social Environment; Social Interaction; Techniques; Testing; Therapeutic; Time; Touch sensation; Training; Work; affiliative behavior; autism spectrum disorder; experience; experimental study; fluorophore; neural; neural correlate; optogenetics; prairie vole; preference; sensor; sex; skills; social attachment; social deficits; social engagement; social relationships; supervised learning; tool; unsupervised learning

PROJECT SUMMARY Romantic bonds are a fundamental part of the human experience, positively contributing to our health and well-being by decreasing risk for physical and mental illness. A key feature of pair bonds - and social relationships more generally - is that they are highly reciprocal, evolving as a function of ongoing feedback from the other individual. Yet the interdependent nature of neural activity within pair bonding remains understudied, due in part to the dearth of laboratory-amenable mammals that form selective attachments with their mating partners. One promising metric for explaining aspects of interaction and its progression in dynamic social contexts is interbrain synchronization, or correlation, in which the neural dynamics of two individuals become more similar to each other as they interact. Interbrain correlation scales with relational variables; it is stronger in romantic partners than strangers during social interaction. I have found that interbrain correlation is also evident in prairie voles and that pair bonded voles show stronger correlations when interacting with their partner than with a stranger. In this proposal, I will leverage modern optical tools to detect and functionally manipulate interbrain correlation in prairie voles. In my first aim, I will perform within-animal comparisons to determine how interbrain correlation varies across discrete behaviors and as a function of pair bonding. I will use fiber-photometry to conduct simultaneous real-time calcium recordings in the medial prefrontal cortex of interacting animals, and use supervised and unsupervised machine learning to conduct thorough behavioral annotations of the interaction. Using a variety of analytical approaches, including generalized linear models, I will delineate the relationship between pair bonding, affiliative behavior and interbrain correlation. In my second aim, I will use synchronized real-time optogenetic stimulation in the mPFC to test whether induced interbrain correlation can facilitate pair bond formation. Together these results will characterize the occurrence of interbrain correlation during distinct behaviors and as a function of bond state and test the function of interbrain correlation in pair bond formation. Such work is instrumental for determining whether therapeutic approaches targeting interbrain correlation will be beneficial for treating disorders that impair social attachment like autism spectrum disorders.

项目概要 浪漫纽带是人类体验的基本组成部分，对我们的健康做出积极贡献 通过降低身体和精神疾病的风险来提高健康水平。结对关系的一个关键特征——社交 更普遍的关系是，它们是高度互惠的，随着持续反馈而不断发展 来自另一个人。然而，配对关系中神经活动的相互依存性质仍然存在 研究不足，部分原因是缺乏适合实验室的哺乳动物，这些哺乳动物与动物形成选择性依恋 他们的交配伙伴。一种有前途的指标，用于解释交互的各个方面及其动态进展 社会情境是脑间同步或相关性，其中两个个体的神经动力学 当他们互动时，彼此会变得更加相似。具有相关变量的脑间相关量表；这是 在社交互动中，浪漫伴侣比陌生人更强。我发现脑间相关性是 在草原田鼠中也很明显，并且成对的田鼠在与它们相互作用时表现出更强的相关性 伙伴胜过与陌生人。在这个提案中，我将利用现代光学工具来检测和功能 操纵草原田鼠的脑间相关性。在我的第一个目标中，我将进行动物内比较 确定脑间相关性如何随着离散行为和配对关系的变化而变化。我会 使用纤维光度测定法对内侧前额叶皮层进行同步实时钙记录 互动动物，并使用有监督和无监督的机器学习来进行彻底的行为 交互的注释。使用各种分析方法，包括广义线性模型，我 将描绘结对关系、亲和行为和脑间相关性之间的关系。在我的第二个 目的，我将在 mPFC 中使用同步实时光遗传学刺激来测试是否诱导脑间 相关性可以促进配对键的形成。这些结果将共同描述发生的情况 不同行为期间的脑间相关性以及作为键状态的函数并测试脑间的功能 成对键形成的相关性。此类工作有助于确定治疗方法是否有效 针对脑间相关性将有助于治疗自闭症等损害社会依恋的疾病 频谱障碍。