REGULATION OF PLACENTAL DEVELOPMENT BY ESTROGEN

雌激素对胎盘发育的调节

• 批准号: 2024827
• 负责人: BILJANA MUSICKI
• 金额: $ 2.61万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2024827
• 关键词: baboons; cell growth regulation; epidermal growth factor; estrogens; fluorescent in situ hybridization; growth factor; hormone receptor; hormone regulation /control mechanism; insulinlike growth factor; northern blottings; placenta; polymerase chain reaction; receptor expression; trophoblast; western blottings; baboons; cell growth regulation; epidermal growth factor; estrogens; fluorescent in situ hybridization; growth factor; hormone receptor; hormone regulation /control mechanism; insulinlike growth factor; northern blottings; placenta; polymerase chain reaction; receptor expression; trophoblast; western blottings

The present study will test the hypothesis that estrogen has a major role in programming the proliferation and/or morphological differentiation of cytotrophoblasts and growth of the placenta during baboon pregnancy through developmental regulation, in an autocrine/paracrine manner, of expression and production of IGF- lI/IGFBP-2, EGF/EGF receptor. Ontogenetic regulation of trophoblast proliferation/ morphological differentiation and growth factor expression will be determined in placentas obtained from baboons on days 45, 60 and 100 of gestation (term = 184 days). The role of estrogen on the same parameters will be studied in placentas obtained from baboons in which estrogen levels have been prematurely elevated early in pregnancy. The temporal effect of estrogen on peptide growth factor expression and trophoblast proliferation/morphological differentiation will be determined in short-term studies (24 and 48 h after injection of androstenedione to baboons on day 45 of pregnancy). Trophoblast proliferation will be assessed by PCNA (index of DNA replication), and morphological differentiation will be assessed by histology and CS production. Quantification and localization of mRNAs and proteins for IGF-II/IGFBP-2, EGF/EGF receptor will be determined by RT-PCR/Northern/Western blot and in situ hybridization, respectively. The proposed study is expected to provide new insight into the developmental regulation of placental growth and differentiation during early primate pregnancy.

本研究将检验雌激素具有主要的假设 在编程扩散和/或形态学中的作用 细胞增生细胞的分化和胎盘的生长 通过发育调节，狒狒怀孕 自分泌/旁分泌方式，IGF-的表达和产生 LI/IGFBP-2，EGF/EGF受体。滋养细胞的个体发生调节 增殖/形态分化和生长因子 表达将在从狒狒获得的胎盘中确定 妊娠的第45、60和100天（术语= 184天）。的作用 将在获得的胎盘中研究相同参数的雌激素 从雌激素水平过高升高的狒狒 怀孕初期。雌激素对肽生长的时间影响 因子表达和滋养细胞增殖/形态学 分化将在短期研究中确定（24和48 h 在怀孕的第45天注射雄性酮向狒狒注射后）。 滋养细胞增殖将通过PCNA评估（DNA指数 复制），形态学分化将由 组织学和CS生产。 mRNA的量化和定位 将确定IGF-II/IGFBP-2，EGF/EGF受体的蛋白质 由RT-PCR/Northern/Western印迹和原位杂交， 分别。拟议的研究预计将提供新的见解 进入胎盘生长的发展调节和 灵长类动物怀孕期间的分化。