SPATIAL EXTENT OF RETINAL DAMAGE

视网膜损伤的空间范围

• 批准号: 2415000
• 负责人: ANN E ELSNER
• 金额: $ 24.53万
• 依托单位: SCHEPENS EYE RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1999-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2415000
• 关键词: aging; biomarker; cell death; choroid uvea; densitometry; digital imaging; early diagnosis; eye disorder diagnosis; eye fundus photography; fluorescein angiography; human middle age (35-64); human old age (65+); human subject; lasers; macular degeneration; pathologic process; retina disorder; retinal pigment epithelium; rod cell; statistics /biometry; visual photoreceptor; young adult human (21-34)

The effects of aging and age-related macular degeneration on photoreceptor function and the supporting retinal pigment epithelial cells is the focus of this proposal. The extent of structural and functional damage across retinal area within retinal and subretinal layers will be probed. We will test the hypothesis that accumulation of subretinaI deposits leads to loss of photoreceptor function. In this context we have two long-term goals: first, to provide a better definition of what is normal aging, as opposed to early signs of disease; second, to understand the role of disease in the death of photoreceptors, including subretinal pathology such as deposits, new vessel membranes, and presence of fluid in detachment. We will determine which portions of the retina are damaged by apparently well-localized vs. diffuse retinal disease processes. This information can be of use for evaluating possible preventative measures, determining the focal effects of treatment, estimating prognosis, and understanding disease processes. To compare structure with function, we use a research Scanning Laser Ophthalmoscope (SLO). To quantify subretinal pathologies we use infra-red imaging to quantify the size and location of sub-retinal features such as deposits, fluid accumulation, and sub-retinal vessels, even through cataract and hemorrhage. To quantify the composition of subretinal pathology, seen with infra-red imaging, and verify its position, we use fluorescein angiography. The location of potential damage to photoreceptors from deposited or exudative material is quantified. The composition of the subretinal pathology will be probed by the pattern of fluorescent binding. To assess the structural integrity of the central cones, their ability to direct light will be quantified with a reflectometric Stiles-Crawford I measurement. To quantify photopigment function, foveal cone and peripheral rod photopigment distribution are measured. Our retinal densitometry measurements are rapid and resistant to stray light. Visual sensitivity at selected retinal loci will be measured with increment threshold. These will be compared with predicted amounts of sensitivity loss from loci of structural damage and amount of photopigment present. Cross sectional studies of clinically normal adults quantify the a) subretinal changes and b) pattern across the retina of photoreceptor loss with aging and in groups at risk for age-related macular degeneration. A statistical definition of normal will be developed for early detection of age-related macular degeneration. We will compare the photopigment loss and structural changes in early and exudative disease in a cross-sectional study and how they are affected by hypertension.

衰老和年龄相关的黄斑变性对感光器的影响 功能和支持的视网膜色素上皮细胞是焦点 该提议。整个结构和功能损害的程度 将探测视网膜和视网膜下层内的视网膜区域。我们将 检验假设的假设，即SubRetinai沉积物的积累导致损失 光感受器功能。在这种情况下，我们有两个长期目标： 首先，为正常衰老提供更好的定义，而不是 早期疾病迹象；第二，了解疾病在 光感受器的死亡，包括视网膜下病理，例如 沉积物，新容器膜以及脱离的液体的存在。我们 将确定视网膜的哪些部分显然损坏了 定位良好的视网膜疾病过程。此信息可以 用于评估可能的预防措施，确定 治疗的焦点效果，估计预后和理解 疾病过程。 为了将结构与功能进行比较，我们使用研究扫描激光器 眼镜镜（SLO）。为了量化视网膜下病理，我们使用了红外线 成像以量化下视网膜特征的大小和位置，例如 即使通过 白内障和出血。 为了量化潜在的视网膜下病理的组成 成像并验证其位置，我们使用荧光素血管造影。这 沉积或渗出性受到感光体的潜在损害的位置 材料被量化。视网膜下病理的组成将 可以通过荧光结合的模式进行探测。评估结构 中央圆锥的完整性，它们的指示能力将是 用反射测量测量值crawford I测量进行量化。 为了量化光谱函数，凹锥和外围棒 测量光谱分布。我们的视网膜光密度法 测量很快，可抵抗杂散的光。视觉灵敏度 选定的视网膜基因座将以增量阈值进行测量。这些 将与预测的敏感性损失量进行比较 存在的结构损伤和存在的光谱量。 临床正常成年人的横截面研究量化了A） 视网膜下变化和b）跨感受器损失的视网膜模式 与年龄相关的黄斑变性的衰老和群体有风险。一个 将开发正常的统计定义，以提早检测 与年龄相关的黄斑变性。我们将比较照片损失 以及横截面早期和渗出性疾病的结构变化 研究以及它们如何受到高血压的影响。