TOPOGRAPHY OF CONE CLASSES

锥体类的地形

• 批准号: 2415020
• 负责人: DAVID R WILLIAMS
• 金额: $ 14.24万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-05-01 至 1998-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2415020
• 关键词: Chelonia; Macaca; brain mapping; charge coupled device camera; color visions; cone cell; human tissue; image enhancement; light intensity; neural information processing; physical model; retina; rod cell; tissue /cell culture; visual fields; visual photoreceptor; visual pigments

Though it has been known that color vision is mediated by three types of photoreceptors for some 200 years, there is little information about the spacing and packing arrangement of the short (S), middle (M), and long (L) wavelength sensitive cones. The spatial organization of the S cones is the best known, while little is known about the relative numbers and distribution of M and L cones. During the last grant period we established the viability of a new technique, Photopigment Transmittance Imaging, to classify cones in the fresh, excised primate retina. Full color images of the photoreceptor mosaic are acquired with a high resolution, high sensitivity CCD camera at light levels that do not bleach substantial amounts of pigment. This allows cones to be typed on the basis of the absorption spectra of their photopigments. The goal of this proposal is to use our imaging technique to map the three interleaved cone submosaics across the monkey and human retina. Such maps may eventually help 1) to understand how trichromacy is incorporated into the retina with minimal cost for spatial vision, 2) to explore the contributions of the three cone types to the post-receptoral channels that are posited from psychophysical experiments, 3) to determine the neural circuitry underlying color vision, and 4) to study the mechanisms responsible for the development of the trichromatic cone mosaic. Our preparation for the classification of cones also allows us to measure the efficiency with which the photoreceptors collect the light incident on the retina. Such measurements will allow the first comprehensive analysis of the fate of photons as they pass through the photoreceptor layer.

尽管众所周知，色觉是由三种类型的 感光器已有200年了，几乎没有关于 短（S），中（M）和长（L）的间距和包装布置 波长敏感锥。 S锥的空间组织是 最著名的，虽然对相对数字和 M和L锥的分布。在最后的赠款期间，我们建立了 一种新技术的生存能力，光化传输成像， 将锥体分类为新鲜，切除的灵长类动物视网膜。全彩色图像 获得感光体的镶嵌物以高分辨率高高地获取 敏感性CCD摄像机的光级别不会变化很大 颜料量。这允许根据 其光鲜摄影的吸收光谱。该提议的目的是 使用我们的成像技术来映射三个交织的锥形子胶质 跨过猴子和人类视网膜。这样的地图最终可能会有所帮助1） 了解如何以最小的方式纳入视网膜 空间视觉的成本，2）探索三个圆锥的贡献 从心理物理中提出的感受后通道的类型 实验，3）确定彩色视觉的神经回路， 4）研究负责发展的机制 三色锥体马赛克。我们为锥体分类的准备 还允许我们测量光感受器的效率 收集视网膜上的灯事件。这样的测量将允许 对光子的命运的首次综合分析 光感受器层。