ShEEP Request for a Multimodal Plate Reader

ShEEP 请求多模式读板仪

• 批准号: 10738976
• 负责人: CHRISTOPHER P CARDOZO
• 金额: --
• 依托单位: JAMES J PETERS VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-01 至 2023-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10738976
• 关键词: Acceleration; Aging; Antibodies; Biochemical Reaction; Biological; Biological Assay; Biomedical Research; Cancer Biology; Chemicals; DNA; Dementia; Devices; Diagnosis; Disease; Enzyme-Linked Immunosorbent Assay; Equipment; Fluorescence; Fluorescence Polarization; Funding; Growth Factor; Health; Hormones; Kidney Diseases; Kinetics; Laboratories; Life; Light; Luciferases; Measurement; Measures; Molecular; Persian Gulf Syndrome; Proteins; Quality of life; RNA; Reader; Research; Research Design; Scanning; Services; Sheep; Specific qualifier value; Spectrophotometry; Spinal cord injury; Temperature; Testing; Time; Traumatic Brain Injury; Veterans; anticancer research; blood pressure control; bone loss; detector; improved; instrument; instrumentation; laptop; light emission; luminescence; molecular dynamics; multimodality; protein protein interaction; Acceleration; Aging; Antibodies; Biochemical Reaction; Biological; Biological Assay; Biomedical Research; Cancer Biology; Chemicals; DNA; Dementia; Devices; Diagnosis; Disease; Enzyme-Linked Immunosorbent Assay; Equipment; Fluorescence; Fluorescence Polarization; Funding; Growth Factor; Health; Hormones; Kidney Diseases; Kinetics; Laboratories; Life; Light; Luciferases; Measurement; Measures; Molecular; Persian Gulf Syndrome; Proteins; Quality of life; RNA; Reader; Research; Research Design; Scanning; Services; Sheep; Specific qualifier value; Spectrophotometry; Spinal cord injury; Temperature; Testing; Time; Traumatic Brain Injury; Veterans; anticancer research; blood pressure control; bone loss; detector; improved; instrument; instrumentation; laptop; light emission; luminescence; molecular dynamics; multimodality; protein protein interaction

The requested instrument is a Molecular Dynamics Spectramax id5 multi-mode Microplate reader with dual injectors. It is used to perform assays based on spectrophotometry, fluorimetry, time- resolved fluorescence and luminescence. This type of instrument is widely used in biomedical research for measuring amounts of certain chemicals such as ATP and of biological substances such as hormones, antibodies and growth factors. It can also be used to test for specific types of protein- protein interactions. It will replace existing though aging equipment that is reaching the end of its service life. It will also be adding capabilities our current instrumentation lacks, such as dual injectors for use in flash assays such as measurement of ATP levels, and the capacity to make measurements using 384-well plates. Its availability will enhance ongoing VA-funded projects focused on reducing bone loss due to spinal cord injury, advancing understanding of the diagnosis and treatment of dementia, greater understanding of Gulf War syndrome and other disorders related to miliary service, traumatic brain injury, renal disease and blood pressure control. By doing so, the equipment will accelerate the pace of VA research aimed at improving health and quality of life of Veterans.

请求的仪器是一种分子动力学Spectramax ID5多模式读取器 带双注射器。它用于根据分光光度法，荧光法，时间 - 进行测定 分辨荧光和发光。这种类型的仪器广泛用于生物医学 研究测量某些化学物质（例如ATP和生物学物质）的研究 作为激素，抗体和生长因子。它也可以用于测试特定类型的蛋白质 - 蛋白质相互作用。它将取代现有的老化设备，该设备已达到其末端 服务寿命。它还将添加我们当前仪器缺乏的功能，例如双向喷油器 用于诸如ATP水平的测量以及进行测量的能力之类的闪光测定法 使用384孔板。它的可用性将增强正在进行的VA资助的项目，以减少降低 由于脊髓损伤引起的骨质流失，推进了对诊断和治疗的理解 痴呆症，对海湾战争综合症的更了解和与媒体服务有关的其他疾病， 创伤性脑损伤，肾脏疾病和血压控制。这样，设备将 加快旨在改善退伍军人健康和生活质量的VA研究速度。