Bioinspired mechanogating biosensors for real-time biodetection

用于实时生物检测的仿生机械生物传感器

• 批准号: 10580157
• 负责人: Jun Yao
• 金额: $ 17.92万
• 依托单位: UNIVERSITY OF MASSACHUSETTS AMHERST
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-03-01 至 2024-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10580157
• 关键词: Area; Binding; Biological; Biological Markers; Biosensing Techniques; Biosensor; Charge; Chemical Interference; Data; Detection; Devices; Diagnosis; Diagnostic; Disease; Early Diagnosis; Electronics; Environment; Exposure to; FDA approved; Future; Goals; Health; Human; Human Bites; Immune response; In Vitro; Intervention; Ionic Strengths; Lyme Disease; Microfluidics; Monitor; Nucleic Acids; Organelles; Pathogen detection; Performance; Physiological; Play; Preparation; Prevention; Public Health; Research; Resolution; Risk; Role; Sampling; Signal Transduction; Silicon; Structure; System; Techniques; Testing; Tick-Borne Diseases; Ticks; Time; Transcend; Transistors; Validation; antibody detection; biochip; clinical diagnostics; design; disease diagnosis; disorder prevention; electrical measurement; fluid flow; improved; mechanotransduction; multiplex detection; nano; nanowire; pathogen; point-of-care diagnostics; prevent; receptor; resilience; screening; sensor; structural imaging; tick bite; tick-borne pathogen; transmission process

Project Summary Electronic biosensors have been playing increasingly important roles in medical diagnostics. Early detections of various biomarkers are desired to provide timely diagnosis for the prevention and treatment of diseases. However, current electronic biosensors are limited in biodetection, often attributed to nonspecific interferences from a complicated ionic environment in bodily solutions. Specifically, charge screening has prevented field-effect biosensors from real-time biodetection in physiological environment. We intend to bring in and validate a new biosensor concept, which can be free of nonspecific charge interference and provide generic solution to the specific biodetection. We also intend to apply the biosensor in the early detection of tick- borne diseases, which have imposed serious threat to public health but lacked means in early detection for timely treatment. Inspired by the mechanotransduction in biological organelles, we will employ a `mechanogating' sensing mechanism that is orthogonal and hence resilient to charge interference. Specifically, we propose to design a biosensor based on a suspended nanotransistor exposed to analyte flow; the binding biomolecules are expected to increase the effective cross-sectional area of the nanotransistor and hence the drag force by the fluid flow; the induced strain will lead to a conductance change through the piezoresistance effect. To realize the goals, in Aim 1 we will assembly and integrate highly suspended nanotransistors as the biosensors specifically designed for the proposed sensing mechanism. In Aim 2, we will evaluate and verify the biosensor function and performance in high ionic strength mimicking the physiological environment. In Aim 3, we will implement the biosensor for the selective detection of pathogens of tick-borne diseases. If successful, the biosensor will provide a practical solution for improved/timely treatment in tick-borne diseases. The research is expected to create a new class of biosensors, which will transcend the inability of field-effect biosensors and realize generic biodetection in physiological environment, leading to advanced biomedical devices for versatile point-of-care diagnostics.

项目摘要 电子生物传感器在医学诊断中发挥了越来越重要的作用。早期的 希望对各种生物标志物的检测提供及时的诊断，以预防和治疗 疾病。但是，当前的电子生物传感器在生物调整方面受到限制，通常归因于非特异性 在身体溶液中的复杂离子环境中干扰。具体来说，电荷筛查具有 在生理环境中阻止了现场效应的生物传感器实时生物调节。我们打算带 在并验证一个新的生物传感器概念，该概念可以免于非特异性电荷干扰并提供 特定生物调整的通用解决方案。我们还打算在早期检测到tick的早期检测中应用生物传感器 - 传播疾病对公共卫生造成了严重威胁，但缺乏早期发现的手段 及时治疗。受到生物细胞器机械转导的启发，我们将采用 正交的“机械化”感应机制，因此可以充电干扰。具体来说， 我们建议根据暴露于分析物流的悬浮纳米递质设计生物传感器。结合 预计生物分子会增加纳米晶体管的有效横截面面积，因此会增加 流体流动的力；诱导的应变将导致通过压电的电导变化 影响。 为了实现目标，在AIM 1中，我们将组建并整合高度悬浮的纳米晶体管作为 专为建议的感应机制而设计的生物传感器。在AIM 2中，我们将评估和验证 模仿生理环境的高离子强度的生物传感器功能和性能。在AIM 3中， 我们将实施生物传感器，以选择性检测tick传播疾病的病原体。如果成功， 生物传感器将为改进/及时治疗tick传播疾病提供实用解决方案。这 预计研究将创建新的生物传感器，这将超越现场效应 生物传感器并在生理环境中实现通用生物调整，从而导致先进的生物医学 多功能诊断的设备。