Component for Institution # 264313

机构组件

基本信息

批准号：
10611924
负责人：
Thomas J Rogers
金额：
$ 18.87万
依托单位：
TEMPLE UNIV OF THE COMMONWEALTH
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-09-30 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10611924
关键词：
Animals Behavior Breeding Cell Line Cells Cloning Collaborations Core Facility Development Discipline Dominant-Negative Mutation Drug Regulations Epitopes Evaluation Funding Gene Expression Gene Expression Profiling Gene Expression Regulation Genes Genotype HIV Immune system In Vitro Institution Investigation Measures Messenger RNA Methodology MicroRNAs Molecular Molecular Analysis Molecular Biology National Institute of Drug Abuse Northern Blotting Nuclear Opioid Receptor Organ Parasites Pharmacotherapy Plasmids Population Procedures Process Proteins Quantitative Reverse Transcriptase PCR RNA Research Research Personnel Research Project Grants Resources Reverse Transcriptase Polymerase Chain Reaction Ribonucleases Rodent Role Run-On Assays Savings Services Signal Transduction Pathway System Techniques Time Tissues Training Transcript Transfection United States National Institutes of Health Universities Work addiction cannabinoid receptor chromatin immunoprecipitation design and construction drug action drug of abuse experience experimental study gene product in vivo interest meetings mutant novel promoter receptor receptor-mediated signaling service utilization trafficking transcription factor vector

项目摘要

The Molecular Biology Core is a critical component of the NIDA P30 Center at Temple University. The Molecular Core offers services to investigators that involve either short- or long- term use of molecular techniques. This Core provides three types of services. First, the Core supports the design and construction of molecular constructs for the expression of genes of interest. This will include, for example, the expression either in vitro or in vivo of opioid or cannabinoid receptors. These molecular constructs will permit a variety of studies of interest to collaborating investigators including the analysis of receptor-mediated signaling processes and the analysis of the cellular trafficking of receptors (or other genes of interest). Second, the Molecular Biology Core will provide gene expression analysis both in vitro and in vivo. The Core provides highly quantitative analysis of mRNA and miRNA expression using RT-PCR, northern blot analysis, RNase protection analysis, and new to this renewal application, the novel FISH flow (PrimeFlow) analysis. The Core also offers comprehensive transcription factor function analyses. Third, the Core provides genotyping services for the Animal Core and to other investigators breeding mutant animals. Services are provided for investigators who span a variety of disciplines and work is carried out in direct collaboration with the investigator. The Molecular Biology Core is particularly interested in continuing efforts to promote the use of molecular techniques to study the role of drugs of abuse in the host-parasite interaction with HIV. The Core has been highly successful in engaging NIH-funded investigators from many disciplines in collaborative research projects that have advanced the understanding of the actions of drugs of abuse and their interplay with the immune system and with HIV. By combining the expertise of investigators with diverse interests, new perspectives and potentially novel investigations can be developed.

分子生物学核心是Temple NIDA P30中心的关键组成部分大学。分子核为调查人员提供服务，涉及短期或长期分子技术的术语使用。该核心提供三种类型的服务。首先，核心支持分子构建体的设计和构建，以表达兴趣。这将包括例如在体外或体内阿片类药物或大麻素受体。这些分子结构将允许各种感兴趣的研究合作研究人员，包括分析受体介导的信号传导过程和分析受体的细胞运输（或其他感兴趣的基因）。第二，分子生物学核心将在体外和体内提供基因表达分析。核心使用RT-PCR，Northern对mRNA和miRNA表达进行高度定量的分析新生鱼的印迹分析，RNase保护分析以及此更新应用的新应用流（PrimeFlow）分析。核心还提供全面的转录因子功能分析。第三，核心为动物核心和其他动物提供基因分型服务研究人员育种突变动物。为跨越的调查人员提供服务各种学科和工作是与调查员直接合作进行的。这分子生物学核心特别感兴趣地继续努力促进使用分子技术研究滥用药物在宿主寄生虫相互作用中的作用艾滋病病毒。核心在吸引许多人的NIH资助的调查员方面非常成功协作研究项目中的学科，使人们对滥用药物及其与免疫系统和艾滋病毒的相互作用的作用。经过将调查人员的专业知识与各种兴趣，新观点以及潜在的专业知识相结合可以开发新颖的调查。