Selenium metabolism in cold-induced adaptive thermogenesis

冷诱导适应性产热中的硒代谢

基本信息

批准号：
10616992
负责人：
Lucia Andreia Seale
金额：
$ 4.81万
依托单位：
UNIVERSITY OF HAWAII AT MANOA
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-01 至 2026-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10616992
关键词：
Adipocytes Administrative Supplement Bioinformatics Brown Fat Cell Death Complement Development Plans Doctor of Philosophy Educational workshop Enzymes Experimental Designs Genes Hispanic Histology Iron Knockout Mice Medical Metabolism Micronutrients Microscopy Oral Parents Recycling Research Research Training Role Selenium Thermogenesis Training Underrepresented Minority Writing adipocyte differentiation career career development collaborative environment experience in vivo meetings mouse model parent grant programs research and development response selenocysteine lyase selenoprotein skills

Adipocytes Administrative Supplement Bioinformatics Brown Fat Cell Death Complement Development Plans Doctor of Philosophy Educational workshop Enzymes Experimental Designs Genes Hispanic Histology Iron Knockout Mice Medical Metabolism Micronutrients Microscopy Oral Parents Recycling Research Research Training Role Selenium Thermogenesis Training Underrepresented Minority Writing adipocyte differentiation career career development collaborative environment experience in vivo meetings mouse model parent grant programs research and development response selenocysteine lyase selenoprotein skills

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项目摘要

ABSTRACT The studies proposed in this Administrative Supplement to parent grant DK128390 aim to promote the scientific research and career development of Sydonie Swanson, a Postbaccalaureate candidate with a Hispanic heritage seeking to gain research experience. The parent grant focuses on determining the role of selenium recycling in regulating selenoprotein enzymes GPX1 and DIO2 in brown adipose tissue and how the recycling mechanism contributes to cold-induced adaptive thermogenesis in vivo, using whole-body and brown adipocyte-specific knockout mouse models of the selenium recycling enzyme selenocysteine lyase (SCLY). This Administrative Supplement will complement these studies by pursuing these Research Aims: 1) To determine the role of selenium and SCLY in the differentiation of brown adipocytes; and 2) To determine if treatment of pre-adipocytes with selenium or the activity of SCLY controls the expression and activity of selenoprotein GPX4, impacting responses to ferroptosis, an iron-dependent cell death mechanism, in differentiated brown adipocytes. The Research and Career Development Plan for Sydonie focuses on training technical (e.g., histology, microscopy, gene editing, bioinformatics) and professional (e.g., experimental design, scientific writing, and oral presentations) skills in a collaborative environment that will provide a robust research experience and favor her successful inclusion in the medical workforce.

抽象的这项行政补充的研究对父母赠款DK128390的旨在促进科学 Sydonie Swanson的研究与职业发展，后者是西班牙裔遗产的后果候选人寻求获得研究经验。父母赠款专注于确定硒回收在调节棕色脂肪组织中的硒蛋白酶GPX1和DIO2以及如何回收机制使用全身和棕色脂肪细胞特异硒回收酶硒代半胱氨酸裂解酶（SCLY）的基因敲除小鼠模型。这个行政补充将通过追求这些研究的目的来补充这些研究：1）确定在棕色脂肪细胞的分化中，硒和scly； 2）确定脂肪细胞的治疗是否使用硒或SCLY的活性控制硒蛋白GPX4的表达和活性，影响对铁铁作用的反应，在分化的棕色脂肪细胞中，铁依赖性细胞死亡机制。这 Sydonie的研究与职业发展计划专注于培训技术（例如，组织学，显微镜，基因编辑，生物信息学）和专业（例如，实验设计，科学写作和口头演讲）在协作环境中的技能，将提供强大的研究经验并偏爱她成功地包括在医疗劳动力中。