Enhancing the Safety, Efficiency, and Research Capacity of the University of Pittsburgh Regional Biocontainment Laboratory to Study Virus Families of Greatest Pandemic Concern

增强匹兹堡大学地区生物防护实验室的安全性、效率和研究能力，以研究最受流行病关注的病毒家族

• 批准号: 10611748
• 负责人: Anantha Shekhar
• 金额: $ 748.1万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-16 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10611748
• 关键词: 2019-nCoV; Address; Air; Animals; Area; Automation; Award; Biology; Biometry; Bunyavirales; COVID-19; Categories; Cities; Communicable Diseases; Coronavirus; Development; Ecosystem; Elements; Ensure; Epidemic; Equipment; Europe; Excision; Family; Ferrets; Fire - disasters; Flaviviridae; Floor; Frequencies; Funding; Future; Gases; Goals; Health; Housing; Human; Individual; Infection; Infectious Agent; Infectious Diseases Research; Infrastructure; Institution; International; Knowledge; Laboratories; Laboratory Research; Lighting; Longevity; Maintenance; Minor; Modernization; Oryctolagus cuniculus; Paramyxovirus; Pennsylvania; Persons; Plasma; Productivity; Public Health; RNA Viruses; Readiness; Research; Research Infrastructure; Research Personnel; Research Project Grants; Rodent; Safety; Schedule; Security; South America; Steam; System; Therapeutic; Time; Togaviridae; Toxin; Translating; Translational Research; United States National Institutes of Health; Universities; University resources; Vaccine Research; Viral; Virus; Walking; Water; Work; animal facility; building renovation; design; exhaust; faculty research; innovation; laboratory facility; medical schools; microbial; nonhuman primate; operation; pandemic disease; pandemic preparedness; pathogen; priority pathogen; programs; recruit; repaired; response; success; time use; tool; vaccine development; vaccine evaluation

Abstract. In 2008, the University of Pittsburgh opened a Regional Biocontainment Laboratory (RBL). Research at the RBL has grown significantly since then, with investigators working on several highly infectious microbial agents requiring BSL2 and ABSL2 facilities. Current RBL research projects cover multiple viruses in 5 of the 7 RNA virus families of pandemic concern as defined by NIH, including, Bunyavirales, Coronaviridae, Flaviviridae, Paramyxoviridae, and Togaviridae. The RBL is an invaluable resource for the University of Pittsburgh, western Pennsylvania, and institutions in the US, Europe and South America. Indeed, the RBL received the SARS-CoV-2 virus in mid-February, 2020, and received almost $5M in March 2020 from the international Coalition for Epidemic Preparedness Innovations to pursue vaccine development for COVID-19. The continued success of the RBL is essential for the US to conduct the necessary research to be prepared for the rapid development and testing of vaccines and therapeutics in response to future pandemics similar to the recent SARS-CoV-2. Over the last 14 years, the infrastructure and facilities in the RBL have begun to degrade, with some reaching the end of their usefulness. The focus of this application is to modernize the RBL through both upgrades and renovations that are designed to allow for continuing cutting-edge research on infectious agents of pandemic concern for many years to come. An effective and efficient RBL is a vital tool for pandemic preparedness. To accomplish our goals, 3 areas of RBL functionality and efficiency will be addressed. First, we will enhance RBL safety and longevity. Repairs or replacements will be made to the bio- containment flooring, the supply air system, the exhaust system, variable frequency drives, chiller controls, steam water heaters, diesel engine generator fuel system controls, fire alarm system, building automation system, walk-in cooler, and biometric security. Second, the efficiency of RBL operations will be enhanced by replacement of existing system lighting with energy-efficient LED lights and removal of a gas plasma sterilizer to generate much needed space. Third, the research capacity of the RBL will be enhanced by increasing rodent housing capacity and purchasing additional caging for non-human primates, rabbits, and ferrets. The ultimate goal of this proposal is to ensure the RBL remains functional, efficient, and ready for rapid response to future emerging pandemics.

抽象的。 2008年，匹兹堡大学开设了一个区域生物疗养实验室（RBL）。 从那时起，RBL的研究就显着增长，研究人员正在研究几个 需要BSL2和ABSL2设施的高度感染性微生物剂。当前的RBL研究 项目涵盖了7种大流行关注的RNA病毒家族中的5个病毒 由NIH，包括Bunyavirales，Coronaviridae，Flaviviridae，Paramyxoviridae和Togaviridae。 RBL是宾夕法尼亚州匹兹堡大学和 美国，欧洲和南美的机构。确实，RBL收到了SARS-COV-2 2020年2月中旬的病毒，并于2020年3月从国际获得了近500万美元 争取Covid-19的疫苗开发的流行病备忘创新联盟。 RBL的持续成功对于美国进行必要的研究至关重要 为响应疫苗和治疗剂的快速开发和测试做好准备 未来的大流行类似于最近的SARS-COV-2。 在过去的14年中，RBL的基础设施和设施开始降级，随着 有些人达到了他们的实用性。该应用程序的重点是现代化RBL 通过旨在继续前沿的升级和翻新 关于流行病的传染病患者的研究多年。有效和 有效的RBL是大流行准备的重要工具。 为了实现我们的目标，将解决RBL功能和效率的3个领域。首先，我们 将提高RBL的安全性和寿命。维修或更换将对生物进行 围栏地板，供应空气系统，排气系统，可变频率驱动器，冷却器 控件，蒸汽热水器，柴油发动机发电机燃油系统控制，火警系统， 建筑自动化系统，步入式冷却器和生物识别安全性。第二，RBL的效率 通过用节能LED替换现有系统照明，将增强操作 灯和去除气体等离子灭菌器可产生急需的空间。第三，研究 通过提高啮齿动物住房能力和购买，将增强RBL的能力 非人类灵长类动物，兔子和雪貂的其他笼子。 该提案的最终目标是确保RBL保持功能，高效和准备 对未来新兴大流行的快速反应。