Rolosense: An innovative platform for automatic mobile phone readout of active SARS-CoV-2 particles

Rolosense：用于自动手机读取活性 SARS-CoV-2 颗粒的创新平台

基本信息

批准号：
10264612
负责人：
Khalid S Salaita
金额：
$ 44.97万
依托单位：
EMORY UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-12-21 至 2022-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10264612
关键词：
2019-nCoV Adhesions Affinity Antibodies Avidity Binding Biological Assay Buffers COVID-19 detection COVID-19 surveillance Caliber Car Phone Cellular Phone Centers for Disease Control and Prevention (U.S.)Complementary RNA DNA Data Device or Instrument Development Diagnostic Engineering Environmental Impact Flushing Geography Goals Hybrids Immobilization Intervention Ionic Strengths Libraries Ligand Binding Mechanics Microfluidic Microchips Microfluidics Motor Mutate Mutation Nebulizer Nucleic Acids Paper Performance Proteins Public Health Publications Publishing RNA Reporting Ribonuclease H Role SARS-CoV-2 spike protein Sensitivity and Specificity Single Nucleotide Polymorphism Specificity Spectrophotometry Speed Structure Surface Technology Temperature Testing Time Transcend Viral Viral Load result Virion Virus Virus Inactivation Work air sampling antibody libraries aptamer commercialization detection limit experience experimental study high reward high risk improved innovation instrumentation millimeter novel particle prototype response screening simulation smartphone Application

2019-nCoV Adhesions Affinity Antibodies Avidity Binding Biological Assay Buffers COVID-19 detection COVID-19 surveillance Caliber Car Phone Cellular Phone Centers for Disease Control and Prevention (U.S.)Complementary RNA DNA Data Device or Instrument Development Diagnostic Engineering Environmental Impact Flushing Geography Goals Hybrids Immobilization Intervention Ionic Strengths Libraries Ligand Binding Mechanics Microfluidic Microchips Microfluidics Motor Mutate Mutation Nebulizer Nucleic Acids Paper Performance Proteins Public Health Publications Publishing RNA Reporting Ribonuclease H Role SARS-CoV-2 spike protein Sensitivity and Specificity Single Nucleotide Polymorphism Specificity Spectrophotometry Speed Structure Surface Technology Temperature Testing Time Transcend Viral Viral Load result Virion Virus Virus Inactivation Work air sampling antibody libraries aptamer commercialization detection limit experience experimental study high reward high risk improved innovation instrumentation millimeter novel particle prototype response screening simulation smartphone Application

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项目摘要

Project Abstract The ultimate goal of this proposal is to develop a novel platform technology for automatic surveillance and tracing of airborne SARS-CoV-2 virus particles in real time. The centerpiece of this proposal is the “Rolosense” technology which leverages a DNA micromotor as the virus sensing and transduction material (VSTM) that can be detected by a conventional smart phone camera. This provides both geographical tracing and surveillance. Rolosense motor are comprised a DNA-coated spherical particle (5 micrometer diameter) that hybridizes to a surface modified with complementary RNA. The particle moves at speeds of over 1 micron/minute upon the addition of RNase H, which selectively hydrolyses hybridized RNA but not single-stranded RNA. DNA motors coated with virus binding ligand (VBL) stall in presence of SARS-CoV-2 virus particles. Because motors move autonomously for distances up to millimeters without intervention, the assay is fully automated, and conventional steps such as viral inactivation, RNA isolation and amplification are not required. The readout is performed using an automated smart phone app for particle tracking without the need for a spectrophotometer or fluorometer. Preliminary data shows realtime SARS-CoV-2 pseudovirus particle sensing. Milestones include the screening and identification of high affinity and high specificity VBLs. Both aptamers and antibody VBLs will be screening and validated. Simulations and experiments will be used to understand the role of temperature and environmental conditions in modulating Rolosense performance. Multivalent display of VBLs with DNA origami will enhance avidity. Finally, microfluidic chips with airborne droplet capture will be implemented and tested. The work will be performed by a highly interdisciplinary team with complementary expertise and a track-record of co-publications. PI Salaita invented the Rolosense technology and has past experience in developing cell phone diagnostics and synthetic motors. Co-I Melikian is an expert virologist, Co-I Heemstra is an expert at developing aptamers for novel targets, Co-I Ke has extensive experience in DNA origami structures for avid target binding and has co-authored work on Rolosense, Co-I Rajaraman and Primordia are experts at microfluidic device development and commercialization. Our solution offers the potential to provide an immediate solution to today’s urgent virus sensing and tracing needs.

项目摘要该提案的最终目标是开发一种新型的平台技术来自动监视并实时追踪机载SARS-COV-2病毒颗粒。该提议的核心是 “ rolosense”技术利用DNA微型运动作为病毒敏感性和转移材料（VSTM）可以通过传统的智能手机相机检测到。这两者都提供地理追踪和监视。 rolosense电动机包含DNA涂层的球形粒子（直径为5微米），与完整的RNA杂交的表面杂交。粒子添加RNase H后，以超过1微米/分钟的速度移动，该rNase H有选择地水解。杂交RNA，而不是单链RNA。涂有病毒结合配体（VBL）失速的DNA电动机在SARS-COV-2病毒颗粒的情况下。因为电动机自动移动以进行距离无干预的毫米，该测定是完全自动化的，常规步骤（例如病毒）失活，不需要RNA分离和扩增。读数是使用用于粒子跟踪的自动化智能手机应用，无需分光光度计或荧光计。初步数据显示实时SARS-COV-2伪病毒颗粒感测。里程碑包括筛选和识别高亲和力和高特异性VBL。既有适体和抗体VBL将进行筛选和验证。模拟和实验将用于理解温度和环境条件在调节rolosense性能中的作用。具有DNA折纸的VBL的多价显示将增强亲和力。最后，带有微流体芯片空降液滴捕获将被实施和测试。这项工作将由高度执行跨学科团队具有完善的专业知识和共同刊登的田径记录。 Pi Salaita 发明了Rolosense技术，并具有开发手机诊断和合成电动机。 Co-I Melikian是一名专家病毒学家，Co-I Heemstra是开发专家适合新目标的适体，Co-I KE在DNA折纸结构方面具有丰富的经验具有约束力，并为Rolosense，Co-I Rajaraman和Primordia共同撰写了作品。微流体设备开发和商业化。我们的解决方案提供了提供的潜力立即解决当今的紧急病毒敏感性和追踪需求。