Physiological and perceptual examination of vision restoration

视力恢复的生理和知觉检查

基本信息

批准号：
10576819
负责人：
William H Merigan
金额：
$ 70.73万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-02-01 至 2025-01-31
项目状态：
未结题

项目摘要

Project summary Despite the current enthusiasm for optogenetic vision restoration, there is remarkably little direct evidence about whether it can reverse blindness in humans. The feasibility of optogenetics was first suggested by studies from many labs that showed restoration of neuronal visual responses and visually guided behaviors in previously blind mice or dogs. These studies have been very informative in exploring interventions to treat retinal diseases genetically identical to some human disorders. However, they have not addressed restoration in an animal model that closely matches human vision, nor have they examined the perceptual quality of the restored vision. In the studies proposed here, we will address these two issues, first by using macaque monkeys in all studies, the animal model that most resembles humans in visual structure and function, and second by examining the range of visual perceptual abilities made possible by optogenetics. We will use a recently developed in-vivo physiology method to study vision restoration at the level of individual retinal cells in macaque monkeys. This approach will be used in the first aim to examine at a cellular level the spatial, temporal and sensitivity responses of macaque retinal neurons produced by optogenetic restoration. In the second aim we will use controlled fixation psychophysical testing of macaques to examine the range of visual capabilities provided by channelrhodopsin restoration, something that has never been studied. Finally, we will examine the duration of optogenetic restoration, measuring any long-term decrease in function or practice-related improvement in visual function.

项目概要尽管目前人们对光遗传学视力恢复充满热情，但关于它是否可以逆转失明的直接证据非常少人类。许多研究首次提出了光遗传学的可行性实验室显示神经元视觉反应和视觉引导的恢复先前失明的老鼠或狗的行为。这些研究非常为探索治疗遗传相同的视网膜疾病的干预措施提供信息某些人类疾病。然而，他们还没有解决恢复问题与人类视觉非常匹配的动物模型，他们也没有检查过恢复视力的感知质量。在这里提出的研究中，我们将解决这两个问题，首先在所有研究中都使用猕猴，在视觉结构和功能上最类似于人类的动物模型，以及其次，通过检查视觉感知能力的范围光遗传学。我们将使用最近开发的体内生理学方法来研究猕猴个体视网膜细胞水平的视力恢复猴子。该方法将用于第一个目标是在细胞水平上进行检查猕猴视网膜神经元的空间、时间和敏感性反应通过光遗传学修复产生。在第二个目标中，我们将使用受控的对猕猴进行注视心理物理测试，以检查视觉范围视紫红质通道恢复提供的功能，这是前所未有的被研究过。最后，我们将检查光遗传学恢复的持续时间，衡量功能的任何长期下降或与实践相关的改善视觉功能。

项目成果

期刊论文数量（3）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Antibody neutralization poses a barrier to intravitreal adeno-associated viral vector gene delivery to non-human primates.

DOI：
10.1038/gt.2014.115
发表时间：
2015-02
期刊：
GENE THERAPY
影响因子：
5.1
作者：
Kotterman, M. A.;Yin, L.;Strazzeri, J. M.;Flannery, J. G.;Merigan, W. H.;Schaffer, D. V.
通讯作者：
Schaffer, D. V.

Restoring vision at the fovea.