Physiological and perceptual examination of vision restoration

视力恢复的生理和知觉检查

基本信息

批准号：
10576819
负责人：
William H Merigan
金额：
$ 70.73万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-02-01 至 2025-01-31
项目状态：
未结题

项目摘要

Project summary Despite the current enthusiasm for optogenetic vision restoration, there is remarkably little direct evidence about whether it can reverse blindness in humans. The feasibility of optogenetics was first suggested by studies from many labs that showed restoration of neuronal visual responses and visually guided behaviors in previously blind mice or dogs. These studies have been very informative in exploring interventions to treat retinal diseases genetically identical to some human disorders. However, they have not addressed restoration in an animal model that closely matches human vision, nor have they examined the perceptual quality of the restored vision. In the studies proposed here, we will address these two issues, first by using macaque monkeys in all studies, the animal model that most resembles humans in visual structure and function, and second by examining the range of visual perceptual abilities made possible by optogenetics. We will use a recently developed in-vivo physiology method to study vision restoration at the level of individual retinal cells in macaque monkeys. This approach will be used in the first aim to examine at a cellular level the spatial, temporal and sensitivity responses of macaque retinal neurons produced by optogenetic restoration. In the second aim we will use controlled fixation psychophysical testing of macaques to examine the range of visual capabilities provided by channelrhodopsin restoration, something that has never been studied. Finally, we will examine the duration of optogenetic restoration, measuring any long-term decrease in function or practice-related improvement in visual function.

项目摘要尽管目前对恢复光遗传学的热情，但仍有关于它是否可以逆转失明的直接证据很少人类。波遗传学的可行性首先是由许多研究提出的显示神经元视觉反应并视觉引导的实验室以前盲小鼠或狗的行为。这些研究非常探索干预措施以治疗视网膜疾病遗传相同的干预措施的信息患有一些人类疾病。但是，他们尚未解决与人类视野紧密相匹配的动物模型，也没有检查恢复视力的感知质量。在这里提出的研究中，我们将首先通过在所有研究中使用猕猴，解决这两个问题，在视觉结构和功能中大多类似于人类的动物模型，并且其次通过检查视觉感知能力的范围光遗传学。我们将使用最近开发的体内生理方法来在猕猴中单个视网膜细胞水平的研究视力恢复猴子。该方法将在第一个目的中用于检查细胞水平猕猴视网膜神经元的空间，时间和灵敏度响应由光遗传学恢复产生。在第二个目标中，我们将使用受控的猕猴的固定心理物理测试，以检查视觉范围 ChannelRhopopsin Restoration提供的功能，从未被研究。最后，我们将检查光遗传恢复的持续时间，测量功能或练习相关的长期降低视觉功能。

项目成果

期刊论文数量（3）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Restoring vision at the fovea.

恢复中央凹的视力。

DOI：
10.1016/j.cobeha.2019.10.003
发表时间：
2019-12
期刊：
Current opinion in behavioral sciences
影响因子：
5
作者：
McGregor JE
通讯作者：
McGregor JE

Antibody neutralization poses a barrier to intravitreal adeno-associated viral vector gene delivery to non-human primates.

抗体中和构成玻璃体内腺相关病毒载体基因递送至非人类灵长类动物的障碍。

DOI：
10.1038/gt.2014.115
发表时间：
2015-02
期刊：
GENE THERAPY
影响因子：
5.1
作者：
Kotterman, M. A.;Yin, L.;Strazzeri, J. M.;Flannery, J. G.;Merigan, W. H.;Schaffer, D. V.
通讯作者：
Schaffer, D. V.