Identifying Differential Psychosocial and Neurobiological Risk Factors of the Transition from Acute to Chronic Pain in Black and Non-­Hispanic White Adults

识别黑人和非西班牙裔白人成人从急性疼痛转变为慢性疼痛的差异心理社会和神经生物学危险因素

• 批准号: 10576262
• 负责人: Bright Eze
• 金额: $ 4.39万
• 依托单位: UNIVERSITY OF CONNECTICUT STORRS
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-02-25 至 2024-02-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10576262
• 关键词: Achievement; Activities of Daily Living; Acute; Address; Adult; Affect; Age; Area; Biological; Black American; Black Populations; Black race; Blood specimen; CREB1 gene; Chronic; Chronic low back pain; Clinical Research; Cohort Studies; DNA Methylation; DNA methylation profiling; Data; Disparity; Ethnic Origin; Event; Exclusion; Exposure to; Female; Foundations; Freezing; Future; Gene Expression; Gene Expression Profile; Genes; Genetic Transcription; Genomics; Goals; Health Sciences; High Prevalence; Incidence; Individual; Inflammation; Inflammation Mediators; Inflammatory; Interleukin-1; Interleukin-6; Intervention; Job Satisfaction; Knowledge; Laboratories; Life; Low Back Pain; Maintenance; Measures; Mentorship; Methods; Moods; Multiomic Data; Musculoskeletal; NF-kappa B; Neurobiology; Not Hispanic or Latino; Pain; Pain Research; Pain Threshold; Pain management; Participant; Pathway interactions; Persons; Phenotype; Physiological; Population; Postdoctoral Fellow; Process; Productivity; Public Health; Quality of life; RNA methylation; Race; Random Allocation; Rehabilitation therapy; Reporting; Research; Risk; Risk Factors; Role; Sampling; Sensory; Severities; Signal Pathway; Site; Socioeconomic Status; Statistical Data Interpretation; Stress; TNF gene; Techniques; Technology; Testing; Time; Traineeship; Training; Tube; United States; Whole Blood; Woman; Work; affective disturbance; age effect; biopsychosocial; black men; black women; career; chronic pain; design; differential expression; disability; ethnic minority population; follow-up; gene environment interaction; health disparity; improved; knowledge integration; low socioeconomic status; mRNA Expression; male; men; neurobiological mechanism; next generation; pain chronification; pain reduction; pain self-management; pain sensitivity; perceived discrimination; perceived stress; preservation; pressure; programs; psychosocial; racial minority population; response; secondary analysis; sex; skills; targeted treatment; theories; Achievement; Activities of Daily Living; Acute; Address; Adult; Affect; Age; Area; Biological; Black American; Black Populations; Black race; Blood specimen; CREB1 gene; Chronic; Chronic low back pain; Clinical Research; Cohort Studies; DNA Methylation; DNA methylation profiling; Data; Disparity; Ethnic Origin; Event; Exclusion; Exposure to; Female; Foundations; Freezing; Future; Gene Expression; Gene Expression Profile; Genes; Genetic Transcription; Genomics; Goals; Health Sciences; High Prevalence; Incidence; Individual; Inflammation; Inflammation Mediators; Inflammatory; Interleukin-1; Interleukin-6; Intervention; Job Satisfaction; Knowledge; Laboratories; Life; Low Back Pain; Maintenance; Measures; Mentorship; Methods; Moods; Multiomic Data; Musculoskeletal; NF-kappa B; Neurobiology; Not Hispanic or Latino; Pain; Pain Research; Pain Threshold; Pain management; Participant; Pathway interactions; Persons; Phenotype; Physiological; Population; Postdoctoral Fellow; Process; Productivity; Public Health; Quality of life; RNA methylation; Race; Random Allocation; Rehabilitation therapy; Reporting; Research; Risk; Risk Factors; Role; Sampling; Sensory; Severities; Signal Pathway; Site; Socioeconomic Status; Statistical Data Interpretation; Stress; TNF gene; Techniques; Technology; Testing; Time; Traineeship; Training; Tube; United States; Whole Blood; Woman; Work; affective disturbance; age effect; biopsychosocial; black men; black women; career; chronic pain; design; differential expression; disability; ethnic minority population; follow-up; gene environment interaction; health disparity; improved; knowledge integration; low socioeconomic status; mRNA Expression; male; men; neurobiological mechanism; next generation; pain chronification; pain reduction; pain self-management; pain sensitivity; perceived discrimination; perceived stress; preservation; pressure; programs; psychosocial; racial minority population; response; secondary analysis; sex; skills; targeted treatment; theories

Abstract Low back pain affects an estimated 65 million individuals in the United States, and Black Americans are disproportionately affected by the detrimental consequences of pain, including a higher incidence of converting to chronic pain, reduced functional capacity, mood, and quality of life compared to Non-Hispanic whites (NHWs). Empirical evidence regarding the Conserved Transcriptional Response to Adversity (CTRA) suggests that adverse psychosocial conditions, such as perceived stress due to experienced discrimination or low socioeconomic status, cause increased expression of genes involved in inflammation and stress-related pathways. While these pathways theoretically overlap with those that influence the transition from acute to chronic pain, the CTRA has never been systematically examined in Black individuals who are at risk of a chronic pain trajectory. This F31 application was designed to provide the applicant with the knowledge and skills to establish a research trajectory in pain-related health disparities. He has garnered the support of a highly engaged and productive mentorship team to fulfill his traineeship goals and accomplish the study aims. The applicant’s overall goals are to: 1) gain a deep understanding of the methods and measures used in pain research; 2) build expertise in health disparities theory and measures; 3) acquire genomics knowledge and principles for carrying out rigorous laboratory techniques with next generation technologies; 4) perform the sequencing pipeline, statistical analysis and interpretation of multi-omic data; and, 5) advance and integrate knowledge of the psychosocial and neurobiological mechanisms of the transition from acute to chronic low back pain in Black Americans. The proposed study is a secondary analysis of a completed inception cohort study (R01NR013932; n=220) that tracked individuals at the onset of acute low back pain and followed them every six weeks for six months. The applicant will randomly select 40 Black and 40 NHW participants (20 men and 20 women each for a total of 80 participants) who developed chronic low back pain. Preserved whole blood samples that were drawn into PAXgene tubes and immediately frozen at -80oC will be processed (baseline acute low back pain and at 6-months/chronic low back pain) for RNA and DNA-methylation (DNAm) sequencing to assess gene x environment interactions. The study aims are to: 1) Identify differences in psychosocial (pain severity and interference, mood, perceived stress, work satisfaction) and neurobiological (quantitative sensory testing) factors; and, 2) Examine differential mRNA expression and DNAm profiles between Black and NHW participants with low back pain at acute onset and at 6-months follow-up. Race x sex comparisons will also be conducted. The proposed study will provide a first-step toward establishing the applicant’s program of research, and will identify unique factors that influence pain-related health disparities in Black individuals that may be used in the future to develop targeted therapy and ethnically and culturally- tailored pain self-management interventions.

抽象的 腰痛影响美国估计有6500万人，黑人美国人是 受到疼痛的不利后果的影响不成比例，包括更高的转化事件 与非西班牙裔白人相比，慢性疼痛，功能能力降低，情绪和生活质量 （NHWS）。关于保守转录反应（CTRA）的经验证据表明 那种不利的社会心理状况，例如由于经历的歧视或低而引起的压力 社会经济状况，导致涉及炎症和压力相关的基因表达增加 途径。这些途径理论上与影响从急性过渡到的那些途径重叠 慢性疼痛，CTRA从未在有可能有危险的黑人中进行系统检查 慢性疼痛轨迹。该F31应用程序旨在为应用程序提供知识和 在与疼痛有关的健康差异方面建立研究轨迹的技能。他获得了 高度参与和产品心态团队实现了他的培训目标并实现了研究目标。 申请人的总体目标是：1）对痛苦中使用的方法和措施有深入的了解 研究; 2）建立健康分配理论和措施方面的专业知识； 3）获取基因组学知识和 使用下一代技术执行严格的实验室技术的原则； 4）执行 测序管道，统计分析和多摩变数据的解释； 5）进步和整合 了解从急性到慢性低的过渡的心理和神经生物学机制 黑人美国人的背痛。 拟议的研究是对完整造成队列的次要分析 研究（R01NR013932; n = 220）在急性下腰痛开始时跟踪个体并跟随它们 每六个星期持续六个月。申请人将随机选择40个黑色和40名NHW参与者（20个人 共有20名女性，共有80名参与者）患有慢性下背部疼痛。保存完整 将加工成-80oc的paxgene管中的血液样本将被处理 （基线急性下腰痛，在6个月/慢性下腰痛时）和DNA-甲基化（DNAM） 评估基因X环境相互作用的测序。研究目的是：1）确定 社会心理（疼痛严重性和干扰，情绪，感知压力，工作满意度）和神经生物学 （定量感觉测试）因素； 2）检查差异mRNA表达和DNAM曲线 在急性发作时的黑色和NHW参与者和6个月的随访之间。种族X性 还将进行比较。拟议的研究将为建立 申请人的研究计划，并将确定影响与疼痛有关的健康分布的独特因素 将来可能会用于开发有针对性疗法以及种族和文化的黑人个体 量身定制的疼痛自我管理干预措施。