Development of a handheld rapid air sensing system to monitor and quantify SARS-CoV-2 in aerosols in real-time

开发手持式快速空气传感系统，实时监测和量化气溶胶中的 SARS-CoV-2

基本信息

批准号：
10273983
负责人：
Ricardo Mancebo
金额：
$ 272.32万
依托单位：
GENENDEAVOR, LLC
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-20 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10273983
关键词：
2019-nCoV Address Aerosols Air Applications Grants Aspirate substance Benchmarking Biophotonics COVID-19 COVID-19 detection COVID-19 monitoring COVID-19 pandemic COVID-19 patient Cell Culture Techniques Cell Nucleus Cessation of life Chemicals Collection Communities Contact Tracing Country DNA Data Detection Development Devices Dose Drops Environment Enzyme Inhibitor Drugs Enzymes Goals Guidelines Handwashing Health Hospitals Hour Human Individual Interdisciplinary Study Investigation Knowledge Laboratories Lead Ligation Lung Masks Measurement Measures Methods Monitor Nasal cavity Onset of illness Performance Photons Polymerase Chain Reaction Predisposition Preparation Process Protocols documentation Quarantine RNA RNA-Directed DNA Polymerase Reaction Time Reading Reagent Reporting Research Research Proposals Reverse Transcription Risk Risk Management Role SARS-CoV-2 infection SARS-CoV-2 transmission Safety Sampling Schools Severity of illness Signal Transduction Social Distance Speed Standardization Symptoms System Technology Testing Time Uncertainty Viral Viral Load result Virus Virus Inactivation Workplace aerosolized air monitoring air sampling base chemical kinetics design detection platform disease transmission epidemiology study foot genomic RNA high risk human coronavirus infection rate infection risk innovation insight knowledge base laboratory equipment monitoring device novel particle photon-counting detector portability prevent real time monitoring sensor solid state electronics tissue culture tool transmission process viral detection

项目摘要

Project Summary The ability to rapidly monitor SARS-CoV-2 in aerosol—drop particles <5 μm in size that evaporate into droplet nuclei and become suspended in air—at the point of presentation is critical to managing the risk of infection by airborne transmission as people return to their communities, workplaces, and schools during the COVID-19 pandemic. However, current enzyme-based methods lack sensitivity, speed, simplicity, and require lab equipment—hence, lack the capability for real-time point-of-presentation (POP) monitoring. In the absence of a real-time POP monitoring capability, SARS-CoV-2 transmission remains poorly understood. In this application, a multidisciplinary research approach that integrates innovations in rapid-kinetic chemical auto-ligation, non- enzymatic isothermal signal amplification, solid-state electronics, and biophotonics is proposed to enable the development of a novel air monitoring system (AMS) that detects and quantifies aerosolized SARS-CoV-2 at the point of presentation in real-time. Recent advances in viral culturing protocols, air sampling technology, and single-photon detection capability will provide the framework for a collaborative research endeavor to establish a new paradigm to address the knowledge gap between the spread of COVID-19 and SARS-CoV-2 aerosol transmission. Therefore, the proposal is aimed at transforming the way COVID-19 is currently researched by providing a tool to enable unparalleled studies that will significantly advance the current knowledgebase. These transformative studies could ultimately guide a new field of investigations that lead to a better understanding of COVID-19 spread, such as viral exposure vs. risk, viral decay rate vs. infectivity, and viral load vs. infectious dose in SARS-CoV-2 airborne transmission. At a minimum, the proposed three research objectives will provide a basic understanding of COVID-19 aerosol transmission. Firstly, current air sampling systems use a multi-step workflow that takes several hours to complete and requires lab equipment, reagents, and significant hands-on time. The goal of objective 1 is to combine air sampling and detection into a one-step real-time POP AMS device that yields SARS-CoV-2 quantification results in less than 5 minutes, without lab equipment or reagents. Secondly, viral inoculum, or initial dose of virus, aspirated into the nasal cavity and lungs has been associated with disease onset and severity. The goal of objective 2 is to optimize and validate AMS to correlate readings from the air monitoring device with tissue-culture infectious dose (TCID50) and reverse transcription polymerase chain reaction (RT-PCR) quantities. These parameters can then later be applied to Human studies to determine the Human infectious dose of SARS-CoV-2 by aerosol transmission. Thirdly, field-based testing in hospitals will provide a means to beta test AMS performance in high-risk environments. The goal of objective 3 is to calibrate AMS measurements with RT-PCR cycle-threshold (Ct) values and cell-culture TCID50 viability results and then benchmark with results from high-risk environments taken from around the world to correlate SARS-CoV-2 aerosol concentrations with global infection rate, as a potential for establishing threshold levels.

项目摘要在气溶胶中快速监测SARS-COV-2的能力 - 大小<5μm的滴颗粒蒸发到Dropret中核并被悬浮在空气中 - 在演示点上，对于管理感染风险至关重要随着人们在Covid-19期间返回社区，工作场所和学校的空气传播，大流行。但是，基于酶的方法缺乏灵敏度，速度，简单设备 - 缺乏实时露天点（POP）监视的能力。实时的POP监测能力，SARS-COV-2传输在此应用中仍然很差。一种多学科研究方法，将创新整合到快速运动化学自动结合中提出了酶等温信号信号放大，固态电子和生物光谱学以使您成为主题开发一种新型的空气监测系统（AMS），该系统检测和量化了雾化的SARS-COV-2 ATT 实时介绍的重点。单光子检测可粘性将主要为建立协作研究的框架一个新的范式来解决Covid-19和Sars-Cov-2气溶胶之间的知识差距因此。提供一种工具来实现非平行的研究，可以显着推进当前的知识库。变革性研究最终可以指导一个新的调查领域，从而更好地理解 COVID-19-19 SARS-COV-2机载传输中的剂量至少为三个互动目标对COVID-19的基本理解，首先是当前的空气符号系统工作流需要几个小时才能完成，需要实验室设备时间。目标1的目标是将空气采样和检测结合在一起产生SARS-COV-2量化的设备在没有实验室设备或试剂的情况下会导致比5分钟少的设备。其次，病毒接种或病毒的初始剂量被吸入鼻腔和肺部。疾病的发作和严重性。从带有组织文化感染剂量（TCID50）的空气监测装置和逆转录聚合酶链反应（RT-PCR）量。通过气溶胶传播确定人类传染剂量的SARS-COV-2。医院将在高风险环境中提供beta测试AMS的手段。是用RT-PCR周期阈值（CT）值和培养物TCID50生存能力校准AM测量结果和蒂马克（Thimark）以及来自世界各地的高风险环境的结果具有全球感染率的SARS-COV-2气溶胶浓度，如建立阈值水平的ASA潜力。