Swarming motility and the regulation of flagellar biosynthesis in Bacillus subtilis

枯草芽孢杆菌的集群运动和鞭毛生物合成的调控

• 批准号: 10582609
• 负责人: Daniel B Kearns
• 金额: $ 48.98万
• 依托单位: TRUSTEES OF INDIANA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10582609
• 关键词: Anabolism; Architecture; Bacillus subtilis; Bacteria; Basic Science; Biochemistry; Cell surface; Cells; Complex; Environment; Flagella; Goals; Gram-Positive Bacteria; Learning; Length; Location; Mediating; Octodon; Pathogenesis; Pattern; Peptidoglycan; Pilum; Positioning Attribute; Proteins; Proteolysis; Regulation; Rod; Rotation; Solid; Stimulus; Surface; System; Thick; Time; Update; Virulence; Work; cell envelope; cell growth; cell motility; forward genetics; nanomachine; response; self organization; spatiotemporal; superresolution microscopy

ABSTRACT Many bacteria are motile by synthesizing corkscrew-like flagella which when rotated propel bacteria through the environment. Each bacterium synthesizes a species-specific number of flagella and inserts the flagella in a species-specific pattern on the cell surface. Flagella are complex nanomachines assembled from dozens of different proteins and how each bacterial species controls flagellar number and patterning is poorly- understood. Moreover, the number of flagella per cell increases when cells come into contact a solid surface to initiate a form of surface motility called swarming. The Kearns lab uses classical forward genetics, super- resolution microscopy, and biochemistry to study flagellar biosynthesis and swarming motility of the Gram positive bacterium Bacillus subtilis. The goals of the project are to understand flagellar biosynthesis in the context of growing cell architecture. First, we will determine how flagellar number is controlled by the poorly- understood master regulator of flagellar biosynthesis SwrA and a response regulator DegU. Second, we will explore how the surface contact response is transduced to inhibit the adaptor-mediated regulatory proteolysis of SwrA and increase flagellar number. Third, we will learn how the flagellar rod insertion through peptidoglycan occurs, and how rod length is controlled to match the thickness of peptidoglycan. Fourth, flagella are synthesized in a grid-like pattern and we will study how flagellar patterning is interpreted and updated in time during cell growth, and coordinated with peptidoglycan insertion. Ultimately, we want to achieve a holistic understanding of how a cell dynamically governs the initiation of flagellar biosynthesis at specific locations to insert the machine through the cell envelope. Our basic research is fundamental to how cells self-organize and is applicable to the spatiotemporal control of the assembly of transenvelope nanomachines involved in pathogenesis including flagella, pili and secretion systems like the injectisome.

抽象的 许多细菌通过合成类似开瓶器的鞭毛是流动的，当旋转的推进细菌时 通过环境。每个细菌都合成了特定物种的鞭毛数量，并插入 鞭毛在细胞表面的物种特异性图案中。鞭毛是复杂的纳米机器 数十种不同的蛋白质以及每个细菌如何控制鞭毛数和图案的方式很差 - 理解。此外，当细胞接触固体表面时，每个细胞的鞭毛数量增加 启动一种称为蜂群的表面运动形式。 Kearns实验室使用经典的前向遗传学，超级 分辨率显微镜和生物化学研究鞭毛生物合成和克的蜂群 细菌阳性枯草菌。该项目的目标是了解鞭毛生物合成 不断增长的细胞体系结构的背景。首先，我们将确定鞭毛号如何由不良的控制 理解了鞭毛生物合成的主调节剂和响应调节剂Degu。第二，我们会的 探索如何转导表面接触响应以抑制适应器介导的调节蛋白水解。 SWRA和增加鞭毛数。第三，我们将学习如何通过鞭毛杆插入 发生肽聚糖，以及如何控制杆长以匹配肽聚糖的厚度。第四，鞭毛 以网格状模式合成，我们将研究如何解释和更新鞭毛图案 细胞生长过程中的时间，并与肽聚糖插入协调。最终，我们想实现整体 了解细胞如何动态控制特定位置的鞭毛生物合成的开始 将机器通过电池信封插入。我们的基础研究是细胞如何自我组织和 适用于涉及跨玻璃纳维罗纳米机器组装的时空控制 发病机理，包括鞭毛，菌毛和分泌系统，例如注射体。