Acquisition of a confocal Raman microscope for molecular fingerprinting of cells and tissue

获取用于细胞和组织分子指纹分析的共焦拉曼显微镜

• 批准号: 10582119
• 负责人: Kyle Patrick Quinn
• 金额: $ 20.05万
• 依托单位: UNIVERSITY OF ARKANSAS AT FAYETTEVILLE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2026-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10582119
• 关键词: Arkansas; Bioenergetics; Biological; Biomedical Research; Cells; Centers of Research Excellence; Complex; Data Science; Development; Disease; Fingerprint; Future; Goals; Heterogeneity; Image; Interdisciplinary Study; Label; Metabolic; Metabolism; Microscope; Microscopy; Molecular; Molecular Profiling; Organism; Oxidation-Reduction; Oxygen; Phase; Pilot Projects; Research; Research Project Grants; Role; Specimen; Spectrum Analysis; Techniques; Tissues; Universities; cellular imaging; light scattering; metabolic imaging; molecular imaging; repaired; spectroscopic imaging; two photon microscopy; vibration

PROJECT SUMMARY The Arkansas Integrative Metabolic Research Center (AIMRC) is a Phase I COBRE at the University of Arkansas at Fayetteville (04/01/2021-02/28/2026; PI: Dr. Kyle P. Quinn). The scientific theme of the AIMRC is to understand the role of cell and tissue metabolism in disease, development, and repair through research involving advanced imaging, bioenergetics, and data science. The long-term goal of the AIMRC is to establish a sustainable interdisciplinary research center that can support biomedical research at the U of A and grow the emerging strength in metabolic research on campus. The Imaging and Spectroscopy Core will enable label-free non-destructive imaging of cellular redox status and oxygen saturation in live cells, tissues, and organisms through an assortment of microscopy and spectroscopy techniques. In addition to our existing capabilities, we propose to install a confocal Raman microscope for non-destructive and label-free imaging of the molecular composition of cells, tissues, and tissue sections. Raman scattering offers the ability to probe biomolecular changes and visualize the complex molecular heterogeneity directly from cells and tissues. The technique relies on the inelastic scattering of light, arising from its interactions with the biological specimen, to quantify the unique vibrational modes of molecules within its native context. Since every molecular species has a unique Raman scattering spectrum or “fingerprint”, analyses of cells and tissues using the Raman microscope can provide highly complementary information to the quantitative metabolic imaging by two-photon microscopy. The Raman microscope purchased as part of this supplement request will be highly beneficial for the projects proposed by current research project leaders and pilot project awardees who may be candidates for future advancement to research project leaders.

项目摘要 阿肯色州综合代谢研究中心（AIMRC）是阿肯色大学的I期cobre 在Fayetteville（04/01/2021-02/28/2026; PI：Kyle P. Quinn博士）。 AIMRC的科学主题是 通过涉及的研究了解细胞和组织代谢在疾病，发育和修复中的作用 高级成像，生物能学和数据科学。 AIMRC的长期目标是建立一个 可持续的跨学科研究中心，可以支持A的生物医学研究并发展 校园代谢研究的新兴力量。成像和光谱核心将无标签 细胞，组织和生物中细胞氧化还原状态和氧饱和度的无损成像 通过各种显微镜和光谱技术。除了我们现有的功能，我们 提议安装共焦拉曼显微镜，以进行分子的非破坏性和无标签成像 细胞，组织和组织切片的组成。拉曼散射具有探测生物分子的能力 直接从细胞和组织中直接改变并可视化复杂的分子异质性。该技术依赖 在光的非弹性散射上，与生物标本的相互作用产生，以量化独特 分子在其本地环境中的振动模式。由于每个分子物种都有独特的拉曼 散射光谱或“指纹”，使用拉曼显微镜对细胞和组织进行分析可以高度提供 通过两光子显微镜的定量代谢成像的互补信息。拉曼 作为此补充要求的一部分购买的显微镜将对由 当前的研究项目负责人和试点项目获奖者可能会成为未来晋升的候选人 研究项目负责人。