Core C: Comparative Pathology Core

核心 C：比较病理学核心

基本信息

批准号：
10573310
负责人：
Enrico Radaelli
金额：
$ 27.89万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-02-15 至 2027-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10573310
关键词：
Address Algorithms Animal Experiments Animal Model Animals Archives Autopsy Biological Biological Markers Biological Process Board Certification Canis familiaris Carbon Cells Classification Clinical Collaborations Collection Comparative Pathology Computer software Consultations Data Dedications Development Ensure Epithelium Evaluation Fibrosis Goals Growth Hematoxylin and Eosin Staining Method Histological Techniques Histology Histopathology Image Analysis Immune Immunofluorescence Immunologic Immunohistochemistry In Situ Hybridization Inflammation Inflammatory Infrastructure Label Laboratories Lesion Macrophage Malignant - descriptor Measurable Modality Modernization Molecular Morphology Mus Normal tissue morphology Outcome Paraffin Embedding Pathologic Pathologist Pathology Patients Phenotype Play Population Pre-Clinical Model Preparation Procedures Protocols documentation Protons Radiation therapy Reproducibility Research Design Research Personnel Rodent Role Route Sampling Services Severities Signal Pathway Sirius Red F3B Slide Solid Specimen Stains Standardization Structure System T-Lymphocyte Techniques Technology Testing Therapeutic Index Time Tissue Sample Tissues Toxic effect Training Translations Trichrome stain method Variant Work animal tissue cytokine digital imaging digital pathology efficacy evaluation experience functional status immune cell infiltrate immunological status interest neoplastic neoplastic cell neutrophil particle pathology imaging personalized approach pre-clinical preclinical study preservation programs response tissue preparation tool translational study tumor tumor microenvironment whole slide imaging

Address Algorithms Animal Experiments Animal Model Animals Archives Autopsy Biological Biological Markers Biological Process Board Certification Canis familiaris Carbon Cells Classification Clinical Collaborations Collection Comparative Pathology Computer software Consultations Data Dedications Development Ensure Epithelium Evaluation Fibrosis Goals Growth Hematoxylin and Eosin Staining Method Histological Techniques Histology Histopathology Image Analysis Immune Immunofluorescence Immunologic Immunohistochemistry In Situ Hybridization Inflammation Inflammatory Infrastructure Label Laboratories Lesion Macrophage Malignant - descriptor Measurable Modality Modernization Molecular Morphology Mus Normal tissue morphology Outcome Paraffin Embedding Pathologic Pathologist Pathology Patients Phenotype Play Population Pre-Clinical Model Preparation Procedures Protocols documentation Protons Radiation therapy Reproducibility Research Design Research Personnel Rodent Role Route Sampling Services Severities Signal Pathway Sirius Red F3B Slide Solid Specimen Stains Standardization Structure System T-Lymphocyte Techniques Technology Testing Therapeutic Index Time Tissue Sample Tissues Toxic effect Training Translations Trichrome stain method Variant Work animal tissue cytokine digital imaging digital pathology efficacy evaluation experience functional status immune cell infiltrate immunological status interest neoplastic neoplastic cell neutrophil particle pathology imaging personalized approach pre-clinical preclinical study preservation programs response tissue preparation tool translational study tumor tumor microenvironment whole slide imaging

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项目摘要

Project Summary The study of the preclinical animal models included in all four Projects plays a critical role to investigate the overall hypothesis that Proton/Carbon Particle FLASH Radiotherapy is superior to Standard Particle Radiotherapy in protecting normal tissues while maintaining equipotent malignant growth control. The extrapolation of robust preclinical data in these experimental settings relies heavily on the unbiased assessment of specific histopathological parameters to investigate the biological effects of FLASH particle radiotherapy as compared to Standard particle radiotherapy on designated neoplastic lesions and adjacent normal tissues. The Comparative Pathology Core (CPC; Core C) will provide its expertise contributing to the planning, evaluation, and interpretation of the pathology endpoints of the animal experiments included in the Projects. As a PennVet clinical laboratory, Core C importantly follows standardized working protocols with strict QA/QC procedures. The histopathology service offered by Core C will ensure accurate collection, proper preservation, timely processing, and staining of the animal specimens. Core C will provide the necessary technical support and submissions of tissue samples will follow a prioritized route. In addition to standard histopathology, Core C will also develop and validate tailored approaches to investigate and quantify tissue changes specifically associated with RT such as fibrosis, intralesional distribution of inflammatory/immune cell populations, expression of markers to evaluate epithelial barrier integrity, etc. The board-certified veterinary pathologists of Core C will deliver expert evaluation and unbiased interpretation of the pathology endpoints. Objective and reproducible quantification of tissue changes in response to the diverse RT modalities will be guaranteed by the application of validated scoring systems and the utilization of software-based algorithms for digital pathology and image analysis. Core pathologists have advanced training in digital imaging pathology from Leica pathology systems. Moreover, Dr. Assenmacher has collaborated with Leica in the development of analysis tools, lending him particular experience in the analytic modules of the digital pathology system.

项目摘要所有四个项目中包含的临床前动物模型的研究起着至关重要的作用总体假设质子/碳颗粒闪光放射疗法优于标准粒子放射疗法在保护正常组织的同时保持等值性恶性生长控制。这在这些实验设置中鲁棒临床前数据的外推在很大程度上取决于公正评估特定的组织病理学参数以研究闪光颗粒的生物学作用放射疗法与指定的肿瘤病变上的标准颗粒放疗相比正常组织。比较病理核心（CPC;核心C）将提供其专业知识，从而有助于对动物实验的病理终点的计划，评估和解释项目。作为Pennvet临床实验室，核心C重要地遵循严格的标准化工作方案 QA/QC程序。 Core C提供的组织病理学服务将确保准确收集，正确动物标本的保存，及时加工和染色。核心将提供必要的组织样本的技术支持和提交将遵循优先的途径。除了标准组织病理学，核心C还将开发和验证量身定制的方法来研究和定量组织与RT特别相关的变化，例如纤维化，炎症/免疫细胞的病内分布种群，评估上皮屏障完整性等标记的表达等。董事会认证的兽医核心C的病理学家将对病理终点进行专家评估和无偏解释。组织变化的客观和可重复的定量响应于不同的RT方式将是通过应用经过验证的评分系统和基于软件的算法的利用来保证用于数字病理和图像分析。核心病理学家接受了数字成像病理学的高级培训来自Leica病理系统。此外，阿森马赫博士与莱卡（Leica）合作开发分析工具，在数字病理系统的分析模块中借用了他的特殊经验。