MULTIDISCIPLINARY PROGRAM IN FEMALE AND MALE REPRODUCTION

女性和男性生殖多学科计划

• 批准号: 7716072
• 负责人: Eugene D. Albrecht
• 金额: $ 22.87万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7716072
• 关键词: Basic Science; Blood Vessels; Cells; Clinical; Clinical Research; Coculture Techniques; Computer Retrieval of Information on Scientific Projects Database; Development; Endometrial; Endometrium; Estrogens; Etiology; Female; Fertility; Functional disorder; Funding; Grant; Human; In Vitro; Infertility; Institution; Knowledge; Maryland; Menstrual cycle; Modeling; Oocytes; Ovarian; Ovary; Papio; Play; Primates; Progesterone; Reproduction; Reproductive Biology; Reproductive system; Research; Research Personnel; Research Project Grants; Resources; Role; Source; Testis; Translating; United States National Institutes of Health; Universities; Vascular remodeling; Virginia; Woman; angiogenesis; fetal; fetal programming; immunocytochemistry; improved; in utero; in vivo; innovation; male; medical schools; men; multidisciplinary; nonhuman primate; programs; receptor; reproductive; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objective of the Specialized Cooperative Centers Program in Reproduction Research (SCCPRR) at the University of Maryland School of Medicine is to conduct innovative basic and clinical research on female and male reproductive biology and to apply the knowledge gained from these studies to improve understanding of the etiology and treatment of reproductive dysfunction and infertility in women and men. The thematic focus of this proposal is the role that estrogen plays in regulating maturation of primate fetal ovaries and testes during intrauterine development and vascular remodeling of the uterine endometrium during the menstrual cycle. In Project I (Gerald J. Pepe), the regulatory role of estrogen in utero in programming fetal ovarian follicular and oocyte development and the impact this has on fertility in adulthood will be assessed in the baboon. In Project II (Eugene D. Albrecht), the regulatory role of estrogen in utero in programming baboon fetal testicular and excurrent ductule/epididymal development and the impact this has on fertility in adulthood will be assessed. A combined clinical-basic research study is proposed in Project III (Robert D. Koos), to determine the role of estrogen and progesterone, acting via expression of angiostimulatory and angioinhibitory factors, on endometrial angiogenesis and vascular remodeling in vivo in baboons and in vitro in cocultures of human endometrial and vascular endothelial and mural cells. We will collaborate with experts in human and nonhuman primate reproductive biology at the University of Maryland SCCPRR, Eastern Virginia Medical School, the University of Pittsburgh SCCPRR, and the Johns Hopkins University SCCPRR. Each of the research projects will draw heavily upon the immunocytochemistry Core (Gloria E. Hoffman) for the cellular localization/expression of regulatory factors and receptors. Integration of the scientific effort in this SCCPRR will be achieved through the thematic focus on estrogen's role in developmental maturation of the reproductive system, use of the baboon as a nonhuman primate model, and an established tradition of collaborative research. The discoveries obtained in the baboon primate model are expected to translate to the human to advance knowledge of human reproductive biology.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 中心，不一定是研究者的机构。 马里兰大学医学院生殖研究专业合作中心项目 (SCCPRR) 的目标是对女性和男性生殖生物学进行创新的基础和临床研究，并应用从这些研究中获得的知识来增进对生殖生物学的理解。女性和男性生殖功能障碍和不孕症的病因和治疗。该提案的主题重点是雌激素在宫内发育过程中调节灵长类胎儿卵巢和睾丸成熟以及月经周期子宫内膜血管重塑中发挥的作用。在项目 I（Gerald J. Pepe）中，将在狒狒身上评估子宫内雌激素对胎儿卵泡和卵母细胞发育的调节作用及其对成年生育力的影响。在项目 II (Eugene D. Albrecht) 中，将评估子宫内雌激素在狒狒胎儿睾丸和外泌管/附睾发育中的调节作用及其对成年生育力的影响。项目 III（Robert D. Koos）提出了一项联合临床基础研究，旨在确定雌激素和孕激素通过血管刺激因子和血管抑制因子的表达对狒狒体内和体外子宫内膜血管生成和血管重塑的作用。在人类子宫内膜和血管内皮细胞和壁细胞的共培养中。我们将与马里兰大学 SCCPRR、东弗吉尼亚医学院、匹兹堡大学 SCCPRR 和约翰霍普金斯大学 SCCPRR 的人类和非人类灵长类动物生殖生物学专家合作。每个研究项目都将大量利用免疫细胞化学核心（Gloria E. Hoffman）来进行调节因子和受体的细胞定位/表达。本次 SCCPRR 科学工作的整合将通过以下主题来实现：雌激素在生殖系统发育成熟中的作用、使用狒狒作为非人类灵长类动物模型以及既定的合作研究传统。在狒狒灵长类动物模型中获得的发现有望转化为人类，以增进人类生殖生物学的知识。