Advanced wireless optogenetics and photometry system for neuroscience research

用于神经科学研究的先进无线光遗传学和光度测量系统

• 批准号: 10240473
• 负责人: Roozbeh Ghaffari
• 金额: $ 99.54万
• 依托单位: NEUROLUX, INC.
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-05-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10240473
• 关键词: 3-Dimensional; Address; Affect; Animal Behavior; Animal Model; Animals; Area; Attention; BRAIN initiative; Behavior; Behavioral; Biological Assay; Biology; Brain; Cannulas; Communities; Complex; Computer software; Country; Detection; Development; Device Designs; Devices; Disadvantaged; Electronics; Engineering; Environment; Feedback; Fiber Optics; Fluorescence; Frequencies; Funding Mechanisms; Goals; Harvest; Implant; Laboratories; Lead; Light; Lighting; Measurement; Measures; Mechanics; Metals; Microfluidics; Mission; Modeling; Modernization; Monitor; Motion; Neurons; Neurosciences; Neurosciences Research; Optics; Output; Peripheral Nervous System; Pharmacology; Pharmacology Study; Phase; Photometry; Physiologic pulse; Price; Program Development; Pump; Quality Control; Research Personnel; Research Support; Running; Scheme; Small Business Innovation Research Grant; Small Business Technology Transfer Research; Social Interaction; Source; System; Talents; Techniques; Technology; Testing; Time; Tissues; Wireless Technology; Work; base; brain research; cost; design; digital; engineering design; experimental study; field study; fluorescence imaging; free behavior; implantable device; improved; in vivo; innovation; irritation; millisecond; miniaturize; neural circuit; operation; optogenetics; prevent; programs; radio frequency; relating to nervous system; research study; response; social group; social movement; software systems; success; tool

Project Summary/Abstract Neuroscience research over the last decade has been revolutionized by many technological advancements. Optogenetics and fluorescence imaging represent two distinct, and sometimes complementary tools used in neuroscience research to study the central and peripheral nervous systems in the context of the BRAIN initiative. Advanced interrogations of underlying neural circuits and biology are often frustrated, however, by technological limitations that prevent the use of these approaches to study natural behaviors of untethered, freely moving animals. Traditional fiber-optic cable for optogenetics and bulky metal cannulas connected with external mechanical pumps for pharmacology impart significant damage to fragile neural tissue, limit the natural behavior of freely moving animals, affect social interactions and movements in complex, naturalistic 3D environment, and lead to persistent irritation at the biotic/abiotic interface due to mechanical mismatch and micromotions. These drawbacks, together with the costly setup, of current technologies motivate the development of innovative engineering designs to improve fidelity, operational ease, versatility and range of advanced brain research studies with live animal models. Our work during Phase II developed capabilities to build our existing system-level hardware and software, as well as scalable manufacturing scheme to fabricate our wireless, battery-free optogenetics devices. Through these efforts, NeuroLux has already achieved substantial success in disseminating tools for neuroscience research, increasing user base to 80 laboratories in 11 countries in the last three years. The proposed work for Phase IIB focuses on further propelling our initial success by refining critical aspects of our hardware, as well as expanding our capabilities to include fully implantable, wireless, battery-free fluorescence imaging to monitor neural activity in real time, in a manner that leverages our current hardware and software. Specifically, the proposed work will (1) develop hardware for multi-enclosure operation with advanced features, (2) advance long- range optogenetic stimulators with active, programmable control over illumination intensity, and (3) develop optoelectronic photometers with options with integrated optogenetic stimulation capabilities.

项目摘要/摘要 在过去十年中，神经科学研究已被许多技术进步彻底改变。 光遗传学和荧光成像代表了两个不同的，有时是互补工具 神经科学研究在大脑倡议的背景下研究中央和周围神经系统。 但是，通过技术，通常会对潜在的神经回路和生物学进行先进的询问 阻止使用这些方法来研究不受限制，自由移动的自然行为的局限性 动物。传统的光纤电缆，用于光遗传学和与外部相连的笨重的金属插管 药理学的机械泵对脆弱的神经组织造成重大损害，限制自然行为 自由移动的动物，影响复杂，自然主义3D环境中的社会互动和运动，以及 由于机械不匹配和微功能，导致生物/非生物界面的持续刺激。这些 当前技术的缺点，以及昂贵的设置，激发了创新的发展 工程设计，以提高忠诚度，运营便利性，多功​​能性和高级大脑研究的范围 使用活动物模型的研究。 我们在第二阶段的工作开发了构建我们现有系统级硬件和软件的功能， 以及可扩展的制造方案，以制造我们的无线无线光遗传学设备。通过 这些努力，Neurolux在传播神经科学工具方面已经取得了巨大成功 研究，在过去三年中，将用户群增加到11个国家的80个实验室。拟议的工作 IIB阶段致力于通过完善硬件的关键方面以及 扩展我们的功能，包括完全植入，无线，无电池荧光成像以监视 实时神经活动，以利用我们当前的硬件和软件的方式。具体来说， 拟议的工作将（1）开发用于具有高级功能的多重操作的硬件，（2）长期提前 具有主动，可编程控制照明强度的范围光遗传刺激器，（3）发展 具有集成光遗传刺激能力的选件的光电子光度计。