Advanced wireless optogenetics and photometry system for neuroscience research

用于神经科学研究的先进无线光遗传学和光度测量系统

• 批准号: 10240473
• 负责人: Roozbeh Ghaffari
• 金额: $ 99.54万
• 依托单位: NEUROLUX, INC.
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-05-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10240473
• 关键词: 3-Dimensional; Address; Affect; Animal Behavior; Animal Model; Animals; Area; Attention; BRAIN initiative; Behavior; Behavioral; Biological Assay; Biology; Brain; Cannulas; Communities; Complex; Computer software; Country; Detection; Development; Device Designs; Devices; Disadvantaged; Electronics; Engineering; Environment; Feedback; Fiber Optics; Fluorescence; Frequencies; Funding Mechanisms; Goals; Harvest; Implant; Laboratories; Lead; Light; Lighting; Measurement; Measures; Mechanics; Metals; Microfluidics; Mission; Modeling; Modernization; Monitor; Motion; Neurons; Neurosciences; Neurosciences Research; Optics; Output; Peripheral Nervous System; Pharmacology; Pharmacology Study; Phase; Photometry; Physiologic pulse; Price; Program Development; Pump; Quality Control; Research Personnel; Research Support; Running; Scheme; Small Business Innovation Research Grant; Small Business Technology Transfer Research; Social Interaction; Source; System; Talents; Techniques; Technology; Testing; Time; Tissues; Wireless Technology; Work; base; brain research; cost; design; digital; engineering design; experimental study; field study; fluorescence imaging; free behavior; implantable device; improved; in vivo; innovation; irritation; millisecond; miniaturize; neural circuit; operation; optogenetics; prevent; programs; radio frequency; relating to nervous system; research study; response; social group; social movement; software systems; success; tool

Project Summary/Abstract Neuroscience research over the last decade has been revolutionized by many technological advancements. Optogenetics and fluorescence imaging represent two distinct, and sometimes complementary tools used in neuroscience research to study the central and peripheral nervous systems in the context of the BRAIN initiative. Advanced interrogations of underlying neural circuits and biology are often frustrated, however, by technological limitations that prevent the use of these approaches to study natural behaviors of untethered, freely moving animals. Traditional fiber-optic cable for optogenetics and bulky metal cannulas connected with external mechanical pumps for pharmacology impart significant damage to fragile neural tissue, limit the natural behavior of freely moving animals, affect social interactions and movements in complex, naturalistic 3D environment, and lead to persistent irritation at the biotic/abiotic interface due to mechanical mismatch and micromotions. These drawbacks, together with the costly setup, of current technologies motivate the development of innovative engineering designs to improve fidelity, operational ease, versatility and range of advanced brain research studies with live animal models. Our work during Phase II developed capabilities to build our existing system-level hardware and software, as well as scalable manufacturing scheme to fabricate our wireless, battery-free optogenetics devices. Through these efforts, NeuroLux has already achieved substantial success in disseminating tools for neuroscience research, increasing user base to 80 laboratories in 11 countries in the last three years. The proposed work for Phase IIB focuses on further propelling our initial success by refining critical aspects of our hardware, as well as expanding our capabilities to include fully implantable, wireless, battery-free fluorescence imaging to monitor neural activity in real time, in a manner that leverages our current hardware and software. Specifically, the proposed work will (1) develop hardware for multi-enclosure operation with advanced features, (2) advance long- range optogenetic stimulators with active, programmable control over illumination intensity, and (3) develop optoelectronic photometers with options with integrated optogenetic stimulation capabilities.

项目概要/摘要 过去十年的神经科学研究因许多技术进步而发生了革命性的变化。 光遗传学和荧光成像代表了两种不同的、有时是互补的工具，用于 神经科学研究，在 BRAIN 计划的背景下研究中枢和周围神经系统。 然而，对底层神经回路和生物学的高级研究常常因技术的原因而受挫。 阻碍使用这些方法来研究不受束缚、自由移动的自然行为的局限性 动物。用于光遗传学的传统光纤电缆和与外部连接的笨重金属插管 用于药理学的机械泵会对脆弱的神经组织造成严重损害，限制自然行为 自由移动的动物，影响复杂、自然的 3D 环境中的社会互动和运动，以及 由于机械不匹配和微运动，导致生物/非生物界面的持续刺激。这些 当前技术的缺点以及昂贵的设置激励了创新技术的发展 工程设计可提高高级大脑研究的保真度、操作简便性、多功能性和范围 使用活体动物模型进行研究。 我们在第二阶段的工作开发了构建现有系统级硬件和软件的能力，例如 以及可扩展的制造方案来制造我们的无线、无电池光遗传学设备。通过 通过这些努力，NeuroLux 已经在传播神经科学工具方面取得了巨大成功 研究，在过去三年中将用户群增加到 11 个国家的 80 个实验室。拟议的工作 IIB 阶段的重点是通过完善硬件的关键方面来进一步推动我们的初步成功，以及 扩展我们的能力，包括完全植入式、无线、无电池荧光成像来监测 以利用我们当前的硬件和软件的方式实时进行神经活动。具体来说， 拟议的工作将（1）开发具有先进功能的多机柜操作硬件，（2）推进长期 系列光遗传学刺激器具有主动、可编程控制照明强度的能力，并且 (3) 开发 光电光度计，具有集成光遗传学刺激功能。