Confocal and Specialized Microscopy Shared Resource

共聚焦和专业显微镜共享资源

• 批准号: 10245178
• 负责人: Liza A Pon
• 金额: $ 12.67万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-04 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10245178
• 关键词: 3-Dimensional; Address; Biological; Biophysics; Cancer Biology; Cancer Center Support Grant; Cancer Model; Cancer Research Project; Cells; Cellular biology; Color; Computer software; Confocal Microscopy; Consult; Consultations; Data; Data Analyses; Doctor of Philosophy; Doctor' s Degree; Ensure; Equipment; Evaluation; Experimental Designs; Fluorescence; Funding; Generations; Goals; Grant; Herbert Irving Comprehensive Cancer Center; Histology; Hour; Human Resources; Image; Imaging Device; Imaging Techniques; Imaging technology; Journals; Laser Scanning Confocal Microscopy; Light; Lighting; Microscope; Microscopy; Nature; Optics; Peer Review; Policies; Proteins; Publications; Reader; Reporting; Reproducibility; Research; Research Project Grants; Research Support; Resolution; Resource Sharing; Resources; Sampling; Scanning; Scientist; Secure; Services; Site; Source; Structure; Surveys; System; Techniques; Technology; Time; Tissues; Training; Training Support; Training and Education; United States National Institutes of Health; Update; Visualization; anticancer research; cancer cell; cellular imaging; community center; cost effectiveness; data acquisition; data visualization; empowered; experience; fluorescence imaging; high resolution imaging; image processing; imaging modality; imaging platform; imaging system; insight; instrument; instrumentation; irradiation; laser capture microdissection; live cell imaging; member; microscopic imaging; new technology; novel; novel imaging technology; operation; programs; prospective; reconstruction; response; second harmonic; service providers; spectrograph; technology development; technology validation; three dimensional structure; tool; two-photon

CONFOCAL AND SPECIALIZED MICROSCOPY SHARED RESOURCE: PROJECT SUMMARY The Confocal and Specialized Microscopy Shared Resource (CSMSR), directed by Liza Pon, PhD, provides access to advanced imaging technologies for Herbert Irving Comprehensive Cancer Center (HICCC) members. Imaging services include: (1) scanning confocal microscopy (including resonant scanning and spectral imaging), (2) two-photon and second-harmonic generation microscopy, (3) spinning-disk confocal microscopy, (4) super-resolution microscopy (Structured Illumination and Stochastic Optical Reconstruction), (5) wide-field fluorescence and color histology imaging, (6) micro-irradiation and laser capture microdissection, and (7) support for image processing, visualization, and quantitative analysis. These comprehensive and integrated services and technologies facilitate analyses of living and fixed cells and tissues in a broad range of cancer models used by HICCC members. During the current reporting period, this state-of-the-art facility updated much of its equipment and incorporated new instruments with support from the HICCC and shared instrumentation grants. This includes acquisition of a cell imaging and multi-mode microplate reader and a multiplate long-term live cell imaging system, as well as major upgrades in Spectral, Resonant-Scanning One- and Two-Photon Confocal and 3D structured illumination super-resolution/scanning confocal microscopes. As result, the CSMSR has expanded its capacity and portfolio of services. In combination with its advanced equipment, highly qualified staff, a broad portfolio of specialized services, easy accessibility, and high cost-effectiveness, the CSMSR is the service provider of choice for HICCC members for advanced imaging. In annual user surveys, the CSMSR is consistently ranked as one of the most highly valued resources at the HICCC. The CSMSR provides services that are not available from commercial sources and not easily accessible at other sites and that are of central importance to the research of numerous HICCC members. In addition to providing both basic and advanced services across different microscopy and imaging platforms, the CSMSR also provides training to HICCC members in the operation of imaging instruments and in image data visualization and analysis. In addition, consulting services in experimental design by expert personnel in the CSMSR facilitate incorporation of advanced imaging techniques into the research portfolio of HICCC members. The CSMSR will continue to incorporate novel imaging platforms and technologies, to provide state-of-the-art imaging services, and to promote high value and accessibility through training and consulting with the goal of empowering the research programs at the HICCC. Over the current project period (2014-2019), the capabilities of the CSMSR were utilized by 66 HICCC members, supported key data and insights facilitating 61 HICCC Member peer-reviewed publications, including 30 contributions in journals of the highest impact factor (IF>10), of which 16 were in journals with and impact factor >20 (e.g., Cell, Nature, Cancer Cell), and currently supports research 44 NIH funded research project grants (25 NCI).

共聚焦和专业显微镜共享资源：项目摘要 由Liza Pon，博士指导的共焦和专业显微镜共享资源（CSMSR）提供 访问Herbert Irving综合癌症中心（HICCC）的高级成像技术 成员。成像服务包括：（1）扫描共聚焦显微镜（包括共振扫描和 光谱成像），（2）两光子和第二谐波生成显微镜，（3）旋转盘共焦 显微镜，（4）超分辨率显微镜（结构化照明和随机光学重建）， （5）宽视野荧光和颜色组织学成像，（6）微辐照和激光捕获 微分解和（7）支持图像处理，可视化和定量分析。这些 全面的综合服务和技术促进了生命和固定细胞的分析，以及 HICCC成员使用的广泛癌症模型的组织。在当前的报告期间， 最先进的设施更新了许多设备，并在 HICCC和共享仪器赠款。这包括获取单元成像和多模式 微板读取器和乘以长期的活细胞成像系统，以及光谱中的重大升级， 谐振的一和两光子共聚焦和3D结构化照明超分辨率/扫描 共聚焦显微镜。结果，CSMSR扩大了其能力和服务组合。在 结合其高级设备，高素质的员工，广泛的专业服务组合，轻松 CSMSR是HICCC成员的首选服务提供商，可访问性和高成本效益 用于高级成像。在年度用户调查中，CSMSR始终被评为最高的 在HICCC上重视资源。 CSMSR提供商业无法获得的服务 来源，在其他站点不容易访问，这对于众多研究至关重要 HICCC成员。除了在不同的显微镜和 成像平台，CSMSR还为HICCC成员提供了成像操作的培训 仪器以及图像数据可视化和分析。此外，在实验中咨询服务 CSMSR中专家人员的设计促进了将高级成像技术纳入 HICCC成员的研究组合。 CSMSR将继续合并新颖的成像平台和 技术，提供最先进的成像服务，并通过 培训和咨询，目的是授权HICCC的研究计划。超过电流 项目期（2014-2019），CSMSR的功能由66位HICCC成员使用，支持 促进61位HICCC成员同行评审出版物的关键数据和见解，包括30个贡献 影响因子最高的期刊（如果> 10），其中16个是在具有影响因素> 20的期刊中（例如， 细胞，自然，癌细胞），目前支持研究44 NIH资助的研究项目补助金（25 NCI）。