High-Parameter Cytometry Shared Resource

高参数细胞分析共享资源

• 批准号: 10239124
• 负责人: Christine Beeton
• 金额: $ 14.17万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10239124
• 关键词: Antibodies; Award; Biology; Cancer Center; Cancer Center Support Grant; Cell Communication; Cell Cycle Progression; Cell Separation; Cell Survival; Cells; Cellular Metabolic Process; Chromatin; Consultations; Core Facility; Cytometry; DNA; Data Analyses; Development; Ensure; Equipment; Evolution; Flow Cytometry; Funding; Future; Generations; Genetic Transcription; Goals; Grant; Growth; Hour; Image; Immune; In Situ; Individual; Inflammation; Laboratories; Letters; Life; Magnetism; Malignant Childhood Neoplasm; Malignant Neoplasms; Medicine; Metals; Mutation; Neoplasm Metastasis; Nuclear Receptors; Oncogenes; Peer Review; Phenotype; Preparation; Protocols documentation; Publications; Reagent; Research; Research Design; Research Institute; Research Personnel; Resource Sharing; Resources; Sampling; Services; Signal Pathway; Signal Transduction; Sorting - Cell Movement; Suspensions; Technology; Texas; Time; Tissue Sample; Tissues; Training; Transcriptional Regulation; Translating; Tumor Suppression; Tumor Suppressor Proteins; Work; angiogenesis; anticancer research; base; cancer cell; cancer prevention; cancer stem cell; cancer therapy; cell type; college; cost effective; data management; experience; imaging platform; imaging system; instrument; instrumentation; malignant breast neoplasm; meetings; member; neoplastic cell; new technology; new therapeutic target; novel; novel therapeutics; particle; prevent; programs; ranpirnase; recruit; repaired; submicron; success; tissue culture; tool; transforming virus; tumor; tumor microenvironment; Antibodies; Award; Biology; Cancer Center; Cancer Center Support Grant; Cell Communication; Cell Cycle Progression; Cell Separation; Cell Survival; Cells; Cellular Metabolic Process; Chromatin; Consultations; Core Facility; Cytometry; DNA; Data Analyses; Development; Ensure; Equipment; Evolution; Flow Cytometry; Funding; Future; Generations; Genetic Transcription; Goals; Grant; Growth; Hour; Image; Immune; In Situ; Individual; Inflammation; Laboratories; Letters; Life; Magnetism; Malignant Childhood Neoplasm; Malignant Neoplasms; Medicine; Metals; Mutation; Neoplasm Metastasis; Nuclear Receptors; Oncogenes; Peer Review; Phenotype; Preparation; Protocols documentation; Publications; Reagent; Research; Research Design; Research Institute; Research Personnel; Resource Sharing; Resources; Sampling; Services; Signal Pathway; Signal Transduction; Sorting - Cell Movement; Suspensions; Technology; Texas; Time; Tissue Sample; Tissues; Training; Transcriptional Regulation; Translating; Tumor Suppression; Tumor Suppressor Proteins; Work; angiogenesis; anticancer research; base; cancer cell; cancer prevention; cancer stem cell; cancer therapy; cell type; college; cost effective; data management; experience; imaging platform; imaging system; instrument; instrumentation; malignant breast neoplasm; meetings; member; neoplastic cell; new technology; new therapeutic target; novel; novel therapeutics; particle; prevent; programs; ranpirnase; recruit; repaired; submicron; success; tissue culture; tool; transforming virus; tumor; tumor microenvironment

PROJECT SUMMARY: High-Parameter Cytometry Shared Resource (HPCSR) Cytometry is an essential and broadly applicable technology for cancer research to assist with elucidating mechanisms of tumor suppressor and oncogenes, cell-cycle progression, transforming viruses, and to evaluate cancer therapies. It is integral to studies of cancer stem cells, angiogenesis, inflammation, transcriptional regulation in tumor cells, and mechanisms of DNA break and repair. The goal of the High-Parameter Cytometry Shared Resource (HPCSR) is to provide a wide range of Cancer Center investigators with access to cost- effective state-of-the-art instrumentation, expertise and training for their cell sorting, and analysis needs. This technology continues to develop at a rapid pace, especially with the advent of novel tools and reagents, increased computational capacity, and more cost-effective equipment. To keep pace with changing technologies, increased utilization, and growth of users over the past 4 years, the HPCSR has expanded its space with new lab renovations, acquired substantial upgrades of existing equipment, and acquired new major equipment utilizing mass, imaging, high-parameter, and large and small particle cytometry. The main facility is housed in centrally located space and is available to trained users 24 hours a day, 7 days a week by key-card access. Current major instrumentation includes a mass cytometer and imaging system, high-parameter fluorescent cytometer, an imaging cytometer, 4 florescence-activated cell sorters, 7 flow analyzers, and magnetic and large particle cell sorters. The Resource is directed by Dr. Christine Beeton and Mr. Joel Sederstrom, whom both have over 20 years of experience in flow cytometry and is staffed by 5 full-time experienced flow cytometrists who perform operator-assisted cytometry and assist users with data analysis. To ensure optimal use of services, HPCSR provides consultations, group and individually tailored training, and protocols for sample preparation, flow analysis, and cell sorting. This is a heavily used Shared Resource that provided service for 119 Cancer Center investigators during the last year, a substantial increase from 87 users in the first year of the CCSG award. It serves all Programs of the DLDCCC with the Programs in Cell Signaling and Metabolism (CSM), Nuclear Receptor, Transcription and Chromatin Biology (NRTCB), Breast Cancer (BCP), Mechanisms in Cancer Evolution (MCEP), and Pediatric Cancer (PCP) being the biggest users of this Shared Resource. This use translates into an average of 50 publications annually. Future plans include development of new technologies and instrumentation for mass, high-parameter, imaging, large and small particle, and spectral cytometry that are pushing the boundaries of conventional cytometry beyond 50 parameters. Further expansion of space and research staff are required for these new technologies and anticipated continued growth of HPCSR.

项目摘要：高参数细胞仪共享资源（HPCSR） 细胞仪是癌症研究的必不可少的技术，可帮助阐明 肿瘤抑制剂和癌基因的机制，细胞周期进展，转化病毒并评估 癌症疗法。它与癌症干细胞，血管生成，炎症，转录的研究不可或缺 调节肿瘤细胞和DNA断裂和修复机制。高参数细胞仪的目标 共享资源（HPCSR）是为了提供广泛的癌症中心调查人员，以获得成本 - 有效的最先进的仪器，专业知识和细胞分类的培训以及分析需求。这 技术继续以快速发展，尤其是随着新颖的工具和试剂的出现， 提高了计算能力和更具成本效益的设备。为了跟上不断变化的技术的步伐， HPCSR的利用率增加和用户的增长，HPCSR通过新的空间扩大了空间 实验室翻新，获得了现有设备的大量升级，并获得了新的主要设备 利用质量，成像，高参数以及大和小粒子细胞仪。主设施安置在 位于中央的空间，每周7天，每天24小时可通过钥匙卡访问提供训练的用户。 当前的主要仪器包括质量细胞仪和成像系统，高参数荧光 细胞仪，一个成像细胞仪，4个荧光激活的细胞分类器，7个流量分析仪以及磁性和大型 粒子细胞分类器。资源由克里斯汀·贝顿（Christine Beeton）博士和乔尔·塞德斯特罗姆（Joel Sederstrom）先生执导，他们都有 超过20年的流式细胞仪经验，由5名全职经验丰富的流式细胞学家组成 执行操作员辅助的细胞仪并帮助用户进行数据分析。为了确保最佳使用服务， HPCSR提供咨询，小组和单独量身定制的培训以及用于样本准备的协议， 流量分析和细胞分类。这是一种大量使用的共享资源，可为119个癌症提供服务 去年中心调查人员，在CCSG的第一年，87位用户大幅增加 奖。它为DLDCCC的所有程序提供了细胞信号传导和代谢方面的程序（CSM）， 核受体，转录和染色质生物学（NRTCB），乳腺癌（BCP），癌症的机制 进化（MCEP）和小儿癌（PCP）是该共享资源的最大用户。此用途 平均每年平均50个出版物。未来计划包括开发新技术 和仪器的质量，高参数，成像，大小粒子和光谱细胞仪的仪器 将常规细胞仪的边界推向50个参数以上。进一步扩展空间和 这些新技术需要研究人员，并预计HPCSR会持续增长。