RESTORATION OF CELL SIGNALING AND MUSCLE PROTEIN SYNTHESIS IN THE ELDERLY

老年人细胞信号传导和肌肉蛋白合成的恢复

基本信息

批准号：
7719198
负责人：
Douglas Paddon-Jones
金额：
$ 3.44万
依托单位：
UNIVERSITY OF TEXAS MED BR GALVESTON
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-04-01 至 2009-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7719198
关键词：
Amino Acids Blood Computer Retrieval of Information on Scientific Projects Database Daily Eating Elderly Experimental Designs Funding Grant Growth Home environment Individual Injury Institution Intervention Invasive Juice Leucine Measures Methodology Monitor Muscle Muscle Proteins Numbers Patients Population Procedures Process Protein Biosynthesis Research Research Personnel Resources Risk Role Sampling Signal Transduction Source Supplementation Techniques Testing Time Treatment Protocols United States National Institutes of Health Water Week cohort day diaries direct application experience human FRAP1 protein novel nutrition restoration stable isotope volunteer

Amino Acids Blood Computer Retrieval of Information on Scientific Projects Database Daily Eating Elderly Experimental Designs Funding Grant Growth Home environment Individual Injury Institution Intervention Invasive Juice Leucine Measures Methodology Monitor Muscle Muscle Proteins Numbers Patients Population Procedures Process Protein Biosynthesis Research Research Personnel Resources Risk Role Sampling Signal Transduction Source Supplementation Techniques Testing Time Treatment Protocols United States National Institutes of Health Water Week cohort day diaries direct application experience human FRAP1 protein novel nutrition restoration stable isotope volunteer

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项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: Older individuals are at greater risk of experiencing a decrease in muscle mass and strength. This may be due to a number of factor, however we are focusing on the potential preventative role of nutrition - specifically a supplement consisting of the amino acid leucine. Hypothesis: We hypothesis that two weeks of leucine supplementation will restore the potential for muscle protein synthesis following a meal that has been shown to be less effective in elderly individuals compared to the young. Specific Aims and Procedures (summary): We will employ techniques to measure muscle protein synthesis (stable isotope methodology) and the mechanisms regulating the synthetic process (mTOR cell signaling) to determine if 2 weeks of leucine supplementation has a beneficial effect the potential for muscle growth in elderly. Experimental Design (summary): We will study a cohort of elderly volunteers over a 14 day period. On days 1 and 14, we will obtain blood and muscle samples to perform our tests. On days 2-13, we will provide volunteers with take-home packets of leucine (4 g each) that they will dissolve in water or juice and consume 3 times/day with their daily meals. We will provide volunteers with a diary to record their normal food intake and monitor their supplement use. Significance (summary): This supplement represents a novel, minimally invasive, preventative intervention that minimizes the additional caloric/volume load associated with previous supplement regimens and has direct application for patient populations and elderly individuals who have a much greater risk of accelerated muscle loss due to illness, injury.

该副本是利用众多研究子项目之一由NIH/NCRR资助的中心赠款提供的资源。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是对于中心，这不一定是调查员的机构。背景：老年人的肌肉质量和力量降低的风险更大。这可能是由于许多因素造成的，但是我们关注营养的潜在预防作用 - 特别是由氨基酸亮氨酸组成的补充剂。假设：我们假设，补充两周的亮氨酸将恢复餐食后的肌肉蛋白质合成的潜力，而这与年轻人相比在老年人中被证明效果较低。具体目的和程序（摘要）：我们将采用技术来测量肌肉蛋白质合成（稳定的同位素方法）和调节合成过程（MTOR细胞信号）的机制来确定补充亮氨酸2周是否具有有益的老年人肌肉生长的有益作用。实验设计（摘要）：我们将在14天的时间内研究一群老年志愿者。在第1和第14天，我们将获得血液和肌肉样本进行测试。在第2-13天，我们将为志愿者提供带回家的亮氨酸（每根4克），它们将溶解在水或果汁中，并每天用餐3次/天。我们将为志愿者提供日记以记录其正常食物摄入量并监测其补充剂的使用。意义（摘要）：该补充剂代表了一种新颖的，微创的预防性干预措施，可最大程度地减少与先前补充剂方案相关的额外热量/体积负荷，并直接适用于患者人群和老年人，这些患者和老年人因疾病造成的肌肉损失而受到更大的肌肉损失风险。