Integrated system for phenotyping energy balance and metabolism in animal models

用于动物模型能量平衡和代谢表型分析的集成系统

• 批准号: 7795396
• 负责人: DANIEL POMP
• 金额: $ 44.47万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-06 至 2011-05-05
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7795396
• 关键词: Advisory Committees; Animal Behavior; Animal Model; Animals; Atherosclerosis; Back; Biological; Body fat; Carbon Dioxide; Charge; Clinical Nutrition; Collection; Complex; Consumption; Core Facility; Data; Diabetes Mellitus; Disease; Disease model; Energy Intake; Energy Metabolism; Environment; Equipment; Evaluation; Exercise; Food; Food Preferences; Funding; Genetic; Genetic Medicine; Genotype; Goals; Home environment; Housing; Imagery; Inbred Mouse; Indirect Calorimetry; Individual; Investigation; Liquid substance; Malignant Neoplasms; Measurement; Mental disorders; Metabolic; Metabolism; Mus; Nutritional Study; Obesity; Online Systems; Pattern; Phenotype; Play; Population; Production; Randomized; Recombinants; Research; Research Personnel; Research Support; Reservations; Resources; Role; Services; Stress; System; Time; Variant; bone mass; drinking; drinking behavior; energy balance; experience; feeding; genome-wide; instrumentation; knockout gene; mouse model; respiratory; tool; trait

DESCRIPTION (provided by applicant): Energy balance, the relationship between energy consumed (through food and drink) and energy expended (through activity and metabolic rate), plays a central role in many diseases, including obesity, diabetes, atherosclerosis, certain cancers and some psychiatric disorders, among others. Mouse models, in various forms such as individual gene knockouts and large, segregating polygenic populations, play a central role in understanding energy balance as a complex trait, including genetic and environmental underpinnings and their interactions. Our long-term objective is to facilitate multifaceted use of mouse models to study energy balance by supporting high quality and high throughput phenotyping of energy balance components, including energy intake, food preference, metabolic rate, home cage activity, voluntary exercise, and serial measurements of body fat, lean mass and bone. Towards this goal, we created an Animal Metabolism Phenotyping (AMP) core facility in 2005 within the NIH-funded Clinical Nutrition Research Center (CNRC) at UNC, and have been providing energy balance phenotyping services to a broad array of NIH-funded biomedical researchers. Our current aim is to significantly expand and upgrade our phenotyping capabilities by placing into the AMP core an integrated 32-cage LabMaster CaloSys (TSE Systems) platform. This includes measurement of indirect calorimetry (O2 consumption & CO2 production, respiratory exchange rate, energy expenditure), feeding and drinking behavior (including amounts and time patterns, food preference, and control of food/liquid access), and spontaneous home cage activity. This system uses the home-cage environment for unbiased stress-free animal behavior, and greatly increases our capabilities to facilitate and support research on a wide variety of diseases that have underpinnings in disregulated energy balance. Thus, use of the requested instrumentation can play a central role in synergizing investigations of many important disease models around one of their important core and common causes. Equipment in the AMP core will be housed in dedicated space within the Vivarium of UNC's new Genetic Medicine Research Building, with capacity for ~45,000 mouse cages. Usage will focus primarily on investigation of a unique new complex trait disease mouse model housed at UNC, the Collaborative Cross (CC), and on evaluation of the consequences of gene knockouts. The CC is a very large panel of recombinant inbred mouse lines and is the only mammalian resource that has high and uniform genome-wide variation effectively randomized across a large, heterogeneous, and reproducible population which also supports integration across environmental and biological conditions, across genotypes, and over time. Equipment use will be streamlined through the experienced CNRC Administrative Core, providing web-based reservations, centralized charge-back billing, and oversight by internal and external advisory committees. Tools developed by UNC's Computational Genetics Group will be used for collection, visualization and dissemination of data.

描述（由申请人提供）：能量平衡，消耗的能量（通过食物和饮料）与消耗的能量（通过活动和代谢率）之间的关系，在许多疾病中起着核心作用，包括肥胖，糖尿病，动脉粥样硬化，某些癌症和某些精神病学疾病。小鼠模型，如各种形式，例如单个基因敲除和大型隔离多基因种群，在理解能量平衡为复杂性状的核心作用，包括遗传和环境基础及其相互作用。我们的长期目标是通过支持能量平衡成分的高质量和高吞吐量表型来促进多方面使用小鼠模型来研究能量平衡，包括能量摄入，食物偏好，代谢率，自愿运动，自愿运动以及对体内脂肪，瘦质量和骨骼的连续测量。为了实现这一目标，我们在2005年在UNC的NIH资助的临床营养研究中心（CNRC）中创建了一个动物代谢表型（AMP）核心设施，并一直为一系列NIH资助的生物医学研究人员提供能源平衡表型服务。我们目前的目的是通过放入一个集成的32座式Labmaster Calosys（TSE Systems）平台来大大扩展和升级表型功能。这包括测量间接量热法（O2消费量和CO2生产，呼吸汇率，能量消耗），喂养和饮酒行为（包括数量和时间模式，食物偏好以及食物/液体访问的控制）以及自发的家居笼子活动。该系统利用自制环境来无偏应有的无压力动物行为，并大大提高了我们促进和支持对各种疾病的研究的能力，这些疾病的基础是无视的能量平衡。因此，使用所请求的仪器可以在对许多重要核心和常见原因之一的许多重要疾病模型协同研究中发挥核心作用。 AMP核心中的设备将安置在UNC新遗传医学研究大楼内的专用空间中，其容量约为45,000小鼠笼。用法将主要集中于对位于UNC，协作十字架（CC）的独特新复杂性状疾病小鼠模型的研究，以及评估基因敲除的后果。 CC是一组非常大的重组近交小鼠系，是唯一具有较高和均匀基因组的哺乳动物资源，在大型，异质性和可重复的种群中有效随机随机随机随机，也支持跨基因型和随着时间的推移以及跨越时间的环境和生物条件的整合。设备的使用将通过经验丰富的CNRC管理核心简化，提供基于网络的预订，集中的收费计费以及内部和外部咨询委员会的监督。 UNC的计算遗传学组开发的工具将用于收集，可视化和传播数据。