In Vivo MicroCT Scanner

体内 MicroCT 扫描仪

• 批准号: 7797486
• 负责人: ROBERTO PACIFICI
• 金额: $ 49.35万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-15 至 2011-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7797486
• 关键词: 1-Phosphatidylinositol 3-Kinase; Acquired Immunodeficiency Syndrome; Animals; Bone Diseases; Brain Neoplasms; Charge; Collaborations; Complement; Data; Estrogens; Funding; Grant; HIV; Hand; Image; In Vitro; Life; Maintenance; Malignant Neoplasms; Measurement; Metastatic Neoplasm to the Bone; Methods; Minor; Modeling; Mus; Organ; Osteogenesis; Osteoporosis; Pharmaceutical Preparations; Research; Research Personnel; Role; Running; Sample Size; Savings; Scanning; Structure; T-Lymphocyte; TNF gene; TNFRSF5 gene; Training; Universities; bone; heart function; improved; in vivo; indexing; instrument; interest; kinase inhibitor; nanoparticle; oncology; operation; programs; public health relevance; tumor growth

DESCRIPTION (provided by applicant): We request funds to purchase a Scanco viva MicroCT model 40, a devise which makes it possible to scan live small animals with the purpose of imaging organs, evaluate heart function, quantify trabecular and cortical bone and obtain information about bone structure. It also allows rapid and accurate measurements of histomorphometric indices of bone volume, connectivity and structure. This instrument will be utilized by 7 major users [Drs. R. Pacifici (P.I.), G. Beck, L.W.K. Chung, M.S. Nanes, Dr. H. Shim M.N. Weitzmann, W. Lewis, who are PI for 7 RO-1s and 1 P0-1, 1 P20, 1 P50 and 1 VA merit grant, and run research programs, on bone, HIV- AIDS, nanoparticles, and cancer at Emory University. In addition, there will be 2 minor users, Drs. D. Durden, and M. Goodman who are P1 of 1 ro1, 1 P50 and a R56 grants, and have interests in oncology. Dr. Robert Guldberg, a collaborator of the P.I. and an expert on MicroCT will be in charge of the maintenance and operations of the instrument. Dr. Pacifici will utilize microCT in studies on the role of T cells in the mechanism of action of PTH and estrogen. Dr. Chung will utilize the microCT to study bone metastasis, Dr. Nanes will use microCT to determine the mechanism by which TNF blocks bone formation. Dr. Shim will utilize the microCT to visualize tumor growth, Dr. Beck and Weitzmann will be aided in their studies on the effects of nanoparticles in bone. Dr. Lewis will use microCT to assess heart function in mice treated with HIV drugs. Dr. Durden will study the effects of PI-3 kinase inhibitors on brain tumors. Dr. Goodman will utilize the microCT to visualize brain tumors. In vivo MicroCT will facilitate each of these projects as it will complement and/or substitute in vitro microCT and bone histomorphometry, and 2D echo for heart function analysis. The advantages of having a microCT are several. 1) It would make possible to expand the planned studies with in vivo data about bone structure, a capability which is currently not on hand. 2) The greater sensitivity and precision of microCT over other methods would results in increased group separation and thus increased ability to interpret the data in a conclusive manner. 3) In some cases it may be possible to decrease the duration of the studies or the sample size with considerable financial savings. 4) We would be able to conduct our studies at a much faster pace. 5) The increased capability of in vivo microCT would provide additional opportunity for collaborations, training of new investigators and opening new fields of research. PUBLIC HEALTH RELEVANCE: Access to an in vivo microCT would allow researchers into causes and cures for osteoporosis and other bone disorders, to expand their studies with data about bone structure from live animals, gain access to greater sensitivity and precision which would results in increased group separation and conclusive interpretation, decrease the duration of studies or the sample size with considerable financial savings, improve the ability to conduct studies at a much faster pace, add additional opportunity for collaborations and training of new investigators which may open new fields of research.

描述（由申请人提供）：我们请求资金购买 Scanco viva MicroCT 型号 40，该设备可以扫描活体小动物，以进行器官成像、评估心脏功能、量化小梁骨和皮质骨并获取有关以下方面的信息：骨骼结构。它还可以快速准确地测量骨体积、连接性和结构的组织形态学指数。该仪器将由 7 个主要用户使用 [Drs. R. Pacifici (P.I.)、G. Beck、L.W.K.钟女士纳内斯 (Nanes)，H. Shim 博士 M.N. Weitzmann、W. Lewis，他们是 7 RO-1 和 1 P0-1、1 P20、1 P50 和 1 VA 奖学金的 PI，并在埃默里大学开展有关骨骼、HIV-AIDS、纳米粒子和癌症的研究项目。此外，还有 2 位未成年用户，Drs。 D. Durden 和 M. Goodman 是 1 ro1 的 P1、1 P50 和 R56 拨款，对肿瘤学感兴趣。罗伯特·古德伯格 (Robert Guldberg) 博士是 P.I. 的合作者。并由MicroCT专家负责仪器的维护和操作。 Pacifici 博士将利用 microCT 研究 T 细胞在 PTH 和雌激素作用机制中的作用。 Chung 博士将利用 microCT 研究骨转移，Nanes 博士将利用 microCT 确定 TNF 阻断骨形成的机制。 Shim 博士将利用 microCT 来观察肿瘤生长，Beck 博士和 Weitzmann 博士将协助他们研究纳米颗粒对骨骼的影响。 Lewis 博士将使用 microCT 来评估接受 HIV 药物治疗的小鼠的心脏功能。 Durden 博士将研究 PI-3 激酶抑制剂对脑肿瘤的影响。 Goodman 博士将利用 microCT 来观察脑肿瘤。体内 MicroCT 将促进这些项目中的每一个，因为它将补充和/或替代体外 microCT 和骨组织形态测量以及用于心脏功能分析的 2D 回波。拥有 microCT 的优点有很多。 1）这将有可能利用有关骨结构的体内数据来扩展计划的研究，而目前尚不具备这种能力。 2) 与其他方法相比，microCT 具有更高的灵敏度和精确度，这会导致组分离增加，从而提高以结论性方式解释数据的能力。 3) 在某些情况下，可以减少研究持续时间或样本量，从而节省大量资金。 4）我们将能够以更快的速度进行研究。 5) 体内 microCT 能力的增强将为合作、培训新研究人员和开辟新的研究领域提供更多机会。 公共健康相关性：获得体内 microCT 将使研究人员能够研究骨质疏松症和其他骨骼疾病的原因和治疗方法，利用活体动物骨骼结构的数据来扩展他们的研究，获得更高的灵敏度和精确度，从而增加群体数量分离和结论性解释，减少研究持续时间或样本量，节省大量资金，提高以更快的速度进行研究的能力，增加合作和培训新研究人员的额外机会，这可能会开辟新的研究领域。