Development of an innovative approach for in situ treatment of PCB impacted sediments by microbial bioremediation

开发一种通过微生物生物修复原位处理受 PCB 影响的沉积物的创新方法

• 批准号: 10077158
• 负责人: Craig Bennett Amos
• 金额: $ 14.03万
• 依托单位: REMBAC ENVIRONMENTAL, LLC
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2021-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10077158
• 关键词: Address; Aerobic; Aerobic Bacteria; Agitation; Anaerobic Bacteria; Area; Bacteria; Biodegradation; Biological Availability; Bioreactors; Bioremediations; Carbon; Cells; Complex; Consumption; Coupled; Culture Media; Deposition; Development; Engineering; Environment; Equipment and supply inventories; Excision; Fishes; Food Chain; Food Webs; Goals; Growth; Harvest; Health; Human; Hydrophobicity; In Situ; Industrialization; Legal patent; Logistics; Longitudinal Studies; Manuals; Mental Depression; Mercury; Methodology; Methods; Microbe; Organism; Performance; Phase; Pilot Projects; Polychlorinated Biphenyls; Preparation; Reporting; Research; Research Personnel; Risk; Site; Source; Surface; System; Technology; Technology Transfer; Testing; Time; Toxic effect; United States; Weather; aqueous; bioaccumulation; cost; cost effective; dechlorination; field study; fundamental research; improved; innovation; landfill; microbial; microorganism; novel; pollutant; receptor; remediation; scale up; time use

Project Summary Polychlorinated biphenyls (PCBs) are one of the most problematic of legacy contaminants. Persistent and mobile in the environment, PCBs are largely ubiquitous in depositional sediments of aquatic systems in industrial regions of the United States. Their relatively high toxicity and bioaccumulation potential cause elevated risk to both human and ecological receptors. As such, PCBs are often the primary risk driver at impacted sediment sites. Common practices for remediating PCB-impacted sediments are costly, often involving the physical removal of surficial sediments, capping the sediments or dredge depression with a multi-layered engineered cap, and disposal of the contaminated sediments in a confined landfill. An emerging strategy for effectively removing PCBs from sediments in-situ is the use of bioamended activated carbon (AC), which inoculates AC pellets with enriched cultures of PCB-degrading microbes. The co-investigators of this proposed research have performed the fundamental research behind the use of bioamended AC for remediation of PCBs from sediment and have developed and patented commercially-viable methods for growing, inoculating, and delivering the inoculated AC pellets to sediments. However, during the performance of pilot-scale studies using the bioamended AC, two factors that would limit the ready use of this technology for large, multi-acre sites were identified: 1) the large-scale growth, storage, and transport of anaerobic PCB degrading bacteria, and; 2) large-scale methods for inoculating AC pellets during application. The proposed research aims to address these limitations. The proposed research will test methods of culturing these organisms using time-release growth media, and develop and test storage vessels that maintain optimal environmental conditions for the long-term viability of the anaerobic organisms. These advances will ultimately allow for the delivery of large volumes of PCB degrading microorganisms for large-scale projects. The proposed research will also develop and test methods for the continuous, uniform inoculation of high volumes of AC pellets with the PCB-degrading microorganisms, which will allow for cost-effective application at multi-acre PCB-impacted sites. Coupled together, the proposed research is anticipated to result in a direct transfer of this technology from pilot-scale to full commercial viability. 1

项目摘要 多氯联苯（PCB）是遗产污染物中最有问题的一种。 PCB在环境中的持久性和移动性在很大程度上无处不在 美国工业地区的水生系统。它们相对较高的毒性和 生物积累的潜在导致人类和生态受体的风险升高。像这样， PCB通常是受影响的沉积物站点的主要风险驱动器。共同的做法 修复PCB影响的沉积物的昂贵，通常涉及物理去除表面 沉积物，用多层工程盖限制沉积物或挖泥凹陷，然后 在受封闭的垃圾填埋场中处理受污染的沉积物。 一种新兴策略，用于有效地从沉积物中删除PCB的原位是生物摄影的使用 活性碳（AC），该碳（AC）用富集的PCB降解培养物接种AC颗粒 微生物。这项拟议的研究的共同研究者进行了基础研究 在使用生物摄影的AC来修复沉积物中的PCB的背后，并开发了 获得专利的商业上可行的方法，用于生长，接种和传递接种的AC 颗粒到沉积物。但是，在使用BioAmended进行试验尺度研究的过程中 AC，这两个因素将限制这项技术在大型多英亩站点中的现成技术使用 确定：1）厌氧PCB降解细菌的大规模生长，存储和运输， 和; 2）在施用过程中接种AC颗粒的大规模方法。拟议的研究 旨在解决这些限制。 拟议的研究将使用时间释放的生长测试培养这些生物的方法 媒体，并开发和测试存储容器，以维持最佳环境条件 厌氧生物的长期生存能力。这些进步最终将允许交付 大量PCB降解大型项目的微生物。拟议的研究 还将开发和测试用于连续均匀接种高体积AC的方法 带有PCB降解微生物的颗粒，这将允许在 占多英亩的PCB撞击站点。结合在一起，预计拟议的研究将导致 将该技术从试点规模转移到完全的商业生存能力。 1