Developing a biomarker panel to assess choline nutritional status

开发生物标志物组来评估胆碱营养状况

基本信息

  • 批准号:
    10066347
  • 负责人:
  • 金额:
    $ 62.77万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2017
  • 资助国家:
    美国
  • 起止时间:
    2017-12-08 至 2022-11-30
  • 项目状态:
    已结题

项目摘要

Choline is an important and essential nutrient, but there are no good validated biomarkers for assessing choline nutritional status that can be practically applied in clinical or public health practice. Choline is a required nutrient with Adequate Intake (AI) and a Tolerable Upper Limit (UL) levels set by the U.S. Institute of Medicine's Food and Nutrition Board and a Recommended Daily Intake (RDI). Given the narrow range for healthy intake of choline, given the establishment of a RDI of choline to maintain health, given the 3-fold variation in dietary intake in the US, given the effects of common genetic variants on requirements for choline and because plasma choline concentrations do not adequately reflect status, we propose that a panel of laboratory tests be developed and validated that assess choline status in humans. From a larger set of measures, we will identify the biomarker measures that best correlate with measurements of choline pool size using isotope dilution. Healthy volunteers (n=60 males, 60 premenopausal females and 60 postmenopausal females), as outpatients, will consume meals containing 100% of the recommended intake of choline (550 mg choline/day) for 2 weeks, they will then be switched to a diet containing 50% of the recommended intake of choline for 2 weeks, then switched to a diet containing 25% of the recommended intake of choline for 2 weeks, and then switched to a diet containing 10% of the recommended intake of choline (55 mg choline) for 2 weeks. Finally, all subjects will be placed on the 100% choline diet for 2 weeks of choline repletion. On the last 3 days of each diet period, subjects will consume deuterated choline. Plasma and urine samples will be collected for metabolomic assays (biomarker small molecules identified in a previous study as likely to predict choline status), isotopic dilution estimation of choline pool size and we will perform transient elastography of liver to assess liver fat. These studies will be used to determine the optimal set of biomarkers to use in calculating a choline status score that correlates well with choline pool size assessed using isotope dilution. Finally, SNPs in genes of choline and folate metabolism have been associated with increased risk for developing organ dysfunction on a low choline diet. We will test our choline status score by determining whether people with these SNPs have a lower choline pool size/worse choline status score for any given choline dietary intake than do people without these SNPs.
胆碱是重要且重要的营养素,但是没有良好的验证生物标志物可以评估胆碱营养状况,这实际上可以用于临床或公共卫生实践中。胆碱是所需的营养素,具有足够的摄入量(AI)和美国医学研究所食品和营养委员会设定的可忍受的上限(UL)水平,并推荐的每日摄入量(RDI)。鉴于在美国饮食摄入量的3倍变化,鉴于胆碱健康摄入胆碱的健康摄入量很小,鉴于常见遗传变异对胆碱的需求和血浆胆碱浓度的需求的影响,因为我们建议的是,我们建议将实验室测试的人群评估choline choline cantans cantans contans contans contans contans consant contans contans contans contans cant antant cant hant ancan choline contancma choline浓度没有充分的反映。从一组较大的措施中,我们将确定使用同位素稀释的胆碱池大小相关的生物标志物测量。 Healthy volunteers (n=60 males, 60 premenopausal females and 60 postmenopausal females), as outpatients, will consume meals containing 100% of the recommended intake of choline (550 mg choline/day) for 2 weeks, they will then be switched to a diet containing 50% of the recommended intake of choline for 2 weeks, then switched to a diet containing 25% of the recommended将胆碱摄入2周,然后切换为含有推荐胆碱摄入量的10%的饮食,持续2周。最后,所有受试者将在100%的胆碱饮食上进行2周的胆碱补充。在每个饮食期的最后3天,受试者将食用氘代胆碱。将收集血浆和尿液样品以进行代谢组学测定(以前的研究中鉴定出的生物标志物小分子可能预测胆碱状态),对胆碱池尺寸的同位素稀释估计,我们将执行肝脏的短暂弹性图以评估肝脏脂肪。这些研究将用于确定用于计算胆碱状态评分的最佳生物标志物集合,该评分与使用同位素稀释度评估的胆碱池尺寸非常相关。最后,胆碱和叶酸代谢基因的SNP与低胆碱饮食中器官功能障碍的风险增加有关。我们将通过确定与没有这些SNP的人相比,任何给定的胆碱饮食摄入量的胆碱池大小/胆碱状态得分较低/胆碱状态得分较差,我们将测试我们的胆碱状态得分。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

暂无数据

数据更新时间:2024-06-01

Steven H Zeisel其他文献

How Nutrigenetics Can Help Prove that Nutrient-Based Interventions Reduce Disease Risk
营养遗传学如何帮助证明基于营养的干预措施可以降低疾病风险
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前往

Steven H Zeisel的其他基金

Developing a biomarker panel to assess choline nutritional status
开发生物标志物组来评估胆碱营养状况
  • 批准号:
    9916222
    9916222
  • 财政年份:
    2019
  • 资助金额:
    $ 62.77万
    $ 62.77万
  • 项目类别:
Administrative Core
行政核心
  • 批准号:
    8132727
    8132727
  • 财政年份:
    2011
  • 资助金额:
    $ 62.77万
    $ 62.77万
  • 项目类别:
Human Requirements for the Nutrient Choline
人类对营养胆碱的需求
  • 批准号:
    7898262
    7898262
  • 财政年份:
    2009
  • 资助金额:
    $ 62.77万
    $ 62.77万
  • 项目类别:
Nutrition Education for Practicing Physicians
执业医师营养教育
  • 批准号:
    8100156
    8100156
  • 财政年份:
    2008
  • 资助金额:
    $ 62.77万
    $ 62.77万
  • 项目类别:
CHOLINE AND NEURODEVELOPMENT IN HUMANS
胆碱与人类神经发育
  • 批准号:
    7716812
    7716812
  • 财政年份:
    2008
  • 资助金额:
    $ 62.77万
    $ 62.77万
  • 项目类别:
HUMAN REQUIREMENTS FOR THE NUTRIENT CHOLINE
人体对营养胆碱的需求
  • 批准号:
    7716848
    7716848
  • 财政年份:
    2008
  • 资助金额:
    $ 62.77万
    $ 62.77万
  • 项目类别:
Nutrition Education for Practicing Physicians
执业医师营养教育
  • 批准号:
    7884608
    7884608
  • 财政年份:
    2008
  • 资助金额:
    $ 62.77万
    $ 62.77万
  • 项目类别:
Nutrition Education for Practicing Physicians
执业医师营养教育
  • 批准号:
    7501031
    7501031
  • 财政年份:
    2008
  • 资助金额:
    $ 62.77万
    $ 62.77万
  • 项目类别:
Nutrition Education for Practicing Physicians
执业医师营养教育
  • 批准号:
    8282993
    8282993
  • 财政年份:
    2008
  • 资助金额:
    $ 62.77万
    $ 62.77万
  • 项目类别:
Nutrition Behavior Intervention Core
营养行为干预核心
  • 批准号:
    7500420
    7500420
  • 财政年份:
    2007
  • 资助金额:
    $ 62.77万
    $ 62.77万
  • 项目类别:

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