Microscopy

显微镜检查

基本信息

批准号：
10022659
负责人：
Deanna L Benson
金额：
$ 11.78万
依托单位：
ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-08-01 至 2025-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10022659
关键词：
Biological Calcium Cancer Center Support Grant Cell Communication Cell Count Cell Lineage Cells Characteristics Collaborations Computer software Computers Consult Consultations Cultured Cells Development Diagnostic Doctor of Philosophy Educational workshop Electron Microscopy Engineering Fluorescence Fluorescence Resonance Energy Transfer Funding Grant Hour Image Image Analysis Immunofluorescence Immunologic Institutes International Laboratories Lead Light Malignant Neoplasms Methodology Microscope Microscopy Mission Modality Neoplasm Metastasis Organ Organoids Paper Primary Neoplasm Productivity Proteins Protocols documentation Publications Publishing Quantitative Microscopy Recording of previous events Research Research Personnel Resolution Resource Sharing Resources Role Schools Science Scientist Series Services Signal Transduction Site Specialist Speed System Techniques Time Tissues Training Training and Education Transmission Electron Microscopy Tumor Cell Migration Tumor-infiltrating immune cells Vendor Work anticancer research base cell behavior cell growth computerized data processing cost design equipment training experience experimental study gigabyte imaging approach imaging study improved in vivo in vivo imaging instrument instrumentation intravital imaging light microscopy medical schools medical specialties member neoplastic cell new technology operation particle programs protein expression skills training training opportunity tumor

项目摘要

PROJECT SUMMARY/ABSTRACT The Tisch Cancer Institute (TCI) Microscopy Shared Resource Facility (MSRF) provides TCI members access to equipment, training, expert consultation, and collaborative opportunities across a full-range of light and electron microscopy applications, including: confocal, multiphoton, super-resolution, in vivo imaging, light sheet, widefield, and transmission electron microscopy (EM). Three high-end workstations equipped with state-of-the- art software accompanied by a local server and high-speed connectivity permit a wide range of image analysis and quantification strategies. Five full time scientists staff the MSRF during regular hours and provide real-time assistance to both novice and experienced users. All of the MSRF scientists can be tapped for implementing advanced approaches. Each holds a PhD or was otherwise trained intensively in a field having particular relevance to their imaging specialty such as super-resolution microscopy or computer engineering. In particular, the EM specialist is a leader in the field and has published over ninety papers covering everything from correlative light-EM (CLEM) to traditional EM techniques, and the scientific advisor is also a TCI member who is expert in intravital imaging approaches and works collaboratively with novice TCI users needing to apply this strategy to cancer-related questions. The MSRF also sponsors monthly seminars featuring scientists using microscopy or vendors developing relevant new technology and its scientists co-direct an annual hands-on microscopy course for graduate school trainees and host workshops throughout the year. The capabilities of the microscope systems together with the skills and training of the team are crucial for supporting TCI studies that include multichannel immunofluorescence for tumor diagnostics, localization of cell signaling and growth regulatory molecules, tumor cell migration and invasion, and cell biological mechanisms of metastasis and dormancy.

项目摘要/摘要 Tisch Cancer Institute（TCI）显微镜共享资源设施（MSRF）为TCI成员提供访问在全面的光线以及电子显微镜应用，包括：共聚焦，多光子，超分辨率，体内成像，轻板，广场和透射电子显微镜（EM）。三个配备了最新的高端工作站伴随当地服务器和高速连接允许的艺术软件允许大量图像分析和量化策略。五个全职科学家在常规时间内员工销售商，并提供实时对新手和经验丰富的用户的帮助。所有的MSRF科学家都可以利用用于实施高级方法。每个人都拥有博士学位或在具有特定的领域进行了深入的训练与他们的成像专业相关，例如超分辨率显微镜或计算机工程。尤其， EM专家是该领域的领导者，已经发表了90篇论文，涵盖了相关性传统EM技术的Light-Em（Clem），科学顾问也是TCI成员，他是专家插入成像方法和与需要将此策略应用于的新手TCI用户合作与癌症有关的问题。 MSRF还赞助了使用显微镜或的科学家的每月研讨会供应商开发相关的新技术及其科学家共同导向年度动手显微镜课程全年为研究生院学员和主办研讨会。显微镜的功能系统以及团队的技能和培训对于支持包括TCI研究至关重要多通道免疫荧光用于肿瘤诊断，细胞信号的定位和生长调节分子，肿瘤细胞迁移和侵袭以及转移和休眠的细胞生物学机制。