Ectonucleotidase modulation of age-dependent vascular calcification and stiffness

外切核苷酸酶调节年龄依赖性血管钙化和僵硬度

• 批准号: 10045790
• 负责人: Nadia Razaq Sutton
• 金额: $ 24.3万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10045790
• 关键词: ADP Receptors; ATP Receptors; Achievement; Adenosine; Age; Age-Years; Aging; Agonist; Arteries; Atherosclerosis; Biological Process; Biology of Aging; Blood Vessels; Brain; C57BL/6 Mouse; Calcium; Cardiac; Cardiovascular Diseases; Cardiovascular system; Cell surface; Cells; Cleaved cell; Clinical; Coagulation Process; Collagen; Coronary artery; Cues; Data; Deposition; Development; Diagnostic radiologic examination; Disease; Elasticity; Elastin; Endothelial Cells; Environment; Enzymes; Exposure to; Extracellular Matrix; Fibrosis; Foundations; Future; Gene Expression; Healthcare; Heart; Human; Impairment; Inflammation; Inflammatory; Kidney; Knowledge; Leukocytes; Life; Longevity; Mediating; Metabolic; Mus; Organ; Osteogenesis; Pathologic; Patients; Phenotype; Physiologic pulse; Physiological; Play; Population; Process; Production; Proteins; Proteomics; Pulse Pressure; Purinergic P1 Receptors; Research; Research Personnel; Risk Factors; Role; Signal Transduction; Smooth Muscle Myocytes; Stress; Stress Tests; Surface; Testing; Time; Vascular Diseases; Vascular Smooth Muscle; Vascular calcification; age related; aged; calcification; care burden; coronary artery calcification; demographics; experimental study; extracellular; human tissue; indexing; inflammatory marker; insight; middle age; mouse model; osteogenic; paracrine; prevent; stem; transcription factor; ADP Receptors; ATP Receptors; Achievement; Adenosine; Age; Age-Years; Aging; Agonist; Arteries; Atherosclerosis; Biological Process; Biology of Aging; Blood Vessels; Brain; C57BL/6 Mouse; Calcium; Cardiac; Cardiovascular Diseases; Cardiovascular system; Cell surface; Cells; Cleaved cell; Clinical; Coagulation Process; Collagen; Coronary artery; Cues; Data; Deposition; Development; Diagnostic radiologic examination; Disease; Elasticity; Elastin; Endothelial Cells; Environment; Enzymes; Exposure to; Extracellular Matrix; Fibrosis; Foundations; Future; Gene Expression; Healthcare; Heart; Human; Impairment; Inflammation; Inflammatory; Kidney; Knowledge; Leukocytes; Life; Longevity; Mediating; Metabolic; Mus; Organ; Osteogenesis; Pathologic; Patients; Phenotype; Physiologic pulse; Physiological; Play; Population; Process; Production; Proteins; Proteomics; Pulse Pressure; Purinergic P1 Receptors; Research; Research Personnel; Risk Factors; Role; Signal Transduction; Smooth Muscle Myocytes; Stress; Stress Tests; Surface; Testing; Time; Vascular Diseases; Vascular Smooth Muscle; Vascular calcification; age related; aged; calcification; care burden; coronary artery calcification; demographics; experimental study; extracellular; human tissue; indexing; inflammatory marker; insight; middle age; mouse model; osteogenic; paracrine; prevent; stem; transcription factor

Abstract With increasing age, blood vessels become stiffer and more calcified. In the latter years of the human lifespan, the process of vascular aging accelerates. The reason that blood vessels lose their youthful elasticity and ability to retard the deposition of calcium precipitously later in life is poorly understood. Ectonucleotidases are found on the surface of endothelial cells which line the inner surface of blood vessels, vascular smooth muscle cells, and leukocytes. The ectonucleotidase CD39 is responsible for cleaving ATP and ADP to form AMP, and subsequently, CD73 is responsible for generating adenosine from AMP. Since ATP and ADP are pro- inflammatory and act in a paracrine fashion, I hypothesize that ectonucleotidase activity plays a role in the vascular stiffness and calcification that occurs as a consequence of age. This is supported by my preliminary data in wild type (C57BL/6) mice, which demonstrates CD73 protein levels declined with age (up to 24 months) in the heart and kidney. This is also supported by preliminary data in mice and human tissues demonstrating that loss of CD73 expression promoted expression of the transcription factor Runx2, which is critical for osteogenesis. We hypothesize that loss of ectonucleotidase expression with age could have deleterious consequences on the vessel wall, resulting in an environment which promotes vascular calcification and stiffness. Since the role of ectonucleotidases in vascular aging is unknown, we will elucidate mechanisms which mediate age-dependent vascular calcification through the following aims. Aim 1: We will determine how age-dependent decline in vascular ectonucleotidase expression renders vessels susceptible to vascular calcification and fibrosis in a murine model. Aim 2: We will determine how ectonucleotidase activity mitigates arterial fibrosis and stiffness. Aim 3: We will determine if ectonucleotidase expression plays a role in age-driven human coronary artery calcification. Achievement of these aims will elucidate the role of ectonucleotidases in age-dependent vascular calcification and stiffness in mice and humans. The mechanistic insights obtained from these experiments will define my future investigative direction and serve as a foundation for a subsequent RO1 application as an independent investigator studying vascular biology and aging.

抽象的 随着年龄的增长，血管变得更硬，更钙化。在人类寿命的后期， 血管衰老的过程加速。血管失去年轻弹性的原因和 人们对钙的沉积能力降低了以后生命的后期沉积的能力。核苷酸酶是 在血管内部表面，血管平滑肌的内表面上发现 细胞和白细胞。 Ectonucleotidase CD39负责分裂ATP和ADP形成AMP，并且 随后，CD73负责从AMP产生腺苷。由于ATP和ADP是Pro- 我假设炎症性和作用于旁分泌方式，肾上腺素酶的活性在 由于年龄而发生的血管僵硬和钙化。这是我的初步支持的 野生型（C57BL/6）小鼠的数据显示CD73蛋白水平随着年龄的增长而下降（长达24个月） 在心脏和肾脏中。这也得到了小鼠和人体组织的初步数据的支持 CD73表达的损失促进了转录因子Runx2的表达，这对于 成骨。我们假设随着年龄的年龄而失去核苷酸酶表达的丧失可能会有有害 容器壁的后果，导致促进血管钙化和 刚性。由于邻核​​酶在血管衰老中的作用尚不清楚，因此我们将阐明机制 通过以下目的介导年龄依赖年龄的血管钙化。目标1：我们将确定如何 血管外核苷酸酶表达的年龄依赖性下降使血管易于血管 鼠模型中的钙化和纤维化。 AIM 2：我们将确定邻核酶的活性如何减轻 动脉纤维化和僵硬。 AIM 3：我们将确定邻核核苷酸酶表达是否在年龄驱动中起作用 人冠状动脉钙化。这些目标的实现将阐明过核苷酸酶在 小鼠和人类的年龄依赖性血管钙化和刚度。获得的机械见解 从这些实验中，将定义我未来的调查方向，并成为随后的基础 RO1应用是研究血管生物学和衰老的独立研究者。