Macromolecular X-ray Diffraction Core Research Facility
高分子X射线衍射核心研究设施
基本信息
- 批准号:10004084
- 负责人:
- 金额:$ 23.07万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-09-01 至 2023-07-31
- 项目状态:已结题
- 来源:
- 关键词:AdoptedAdvisory CommitteesAreaBacteriaBiochemicalBiologicalBiomedical ResearchBiophysicsCellsCenters of Research ExcellenceCloningCollaborationsCommunitiesConsultationsCrystallizationCrystallographyDataEducational workshopEvaluationFacultyFee SchedulesFee-for-Service PlansFeesFellowship ProgramGenesHuman ResourcesImageInfrastructureInsectaLaboratoriesLibrariesLiquid substanceManualsMeasurementMeasuresMethodsMissionModelingMolecularMolecular StructureMontanaNitrogenPhaseProceduresProteinsProtocols documentationRNARecombinant ProteinsReportingResearchResearch PersonnelResearch TrainingResolutionResourcesRoboticsRoentgen RaysScreening procedureServicesSourceStrategic PlanningStructureStudentsSynchrotronsSystemTechniquesTechnologyTrainingTraining and EducationUniversitiesVisualizationX ray diffraction analysisX-Ray Crystallographybasecomputerized data processingcomputing resourcesdesigndetectorexperimental studyinstrumentinstrumentationlecturesmacromoleculememberpractical applicationprotein expressionresearch facilityrobot assistancesmall moleculethree dimensional structuretoolweb site
项目摘要
The CBSD Macromolecular X-ray Diffraction Core (MXDC) provides support, expertise, and infrastructure to
CBSD COBRE Investigators and University of Montana laboratories that use X-ray crystallography as a tool to
determine the three-dimensional structures of small molecules and biological macromolecules, and seek to
express and purify proteins and RNA for biochemical, biophysical and structural studies. The MXDC
possesses the infrastructure and expertise to provide comprehensive user access to shared major
instrumentation and research training for expression of recombinant proteins in bacteria and insect cells, robot-
assisted crystallization of macromolecules, evaluation of crystallization experiments, and measurement of
diffraction data from crystals of both small and macromolecules and determination of their three-dimensional
structures at atomic or near-atomic resolution. The facility houses a Rigaku 007HF X-ray generator and R-axis
image plate detector for macromolecular crystallography and a Bruker D8 Venture fixed Chi dual source
diffractometer for small molecule applications. Both instruments feature Oxford liquid-nitrogen crystal cryo-
cooling-systems. The MXDC routinely measures X-ray diffraction data from crystals at synchrotron sources at
single and multiple wavelengths for structure determination by molecular replacement, heavy atom, and
anomalous dispersion phasing. Computational resources of the MXDC are also available for X-ray data
processing, structure determination, refinement, and visualization for both small-molecule and macromolecular
structure determination. The MXDC encourages a collaborative model, in which faculty, students and staff
conduct projects with assistance and training from Core personnel. The MXDC is a resource for training,
education, consultation and collaboration in protein expression/purification and both small molecule and
macromolecular X-ray crystallographic techniques. The specific aims of this component of the Phase II CBSD
COBRE are: 1, to oversee and maintain a productive collaborative facility by adopting procedures for project
planning, an appropriate fee structure and project reporting mechanism, and both short-term and strategic
planning with the guidance of an Advisory Committee; 2, to maintain and enhance gene-to-structure and small
molecule structure determination services by implementing state-of-the-art technologies; 3, to pursue research
collaborations with CBSD COBRE investigators in aspects of their projects that involve areas of MXDC
expertise; and 4, to provide training in practical application of techniques in protein expression, purification,
crystallization, and X-ray structure determination (macro and small-molecule) for CBSD investigators and UM
faculty through direct collaborations, workshops, lectures, the MXDC website and the MDXC Core Fellowship
Program.
1
CBSD 高分子 X 射线衍射核心 (MXDC) 提供支持、专业知识和基础设施
CBSD COBRE 研究人员和蒙大拿大学实验室使用 X 射线晶体学作为工具
确定小分子和生物大分子的三维结构,并寻求
表达和纯化蛋白质和 RNA,用于生化、生物物理和结构研究。 MXDC
拥有基础设施和专业知识,可以为用户提供对共享专业的全面访问
细菌和昆虫细胞中重组蛋白表达的仪器和研究培训,机器人
大分子的辅助结晶、结晶实验的评估和测量
小分子和大分子晶体的衍射数据及其三维测定
原子或近原子分辨率的结构。该设施配有 Rigaku 007HF X 射线发生器和 R 轴
用于大分子晶体学的图像板检测器和 Bruker D8 Venture 固定 Chi 双源
适用于小分子应用的衍射仪。两种仪器均采用 Oxford 液氮晶体冷冻技术
冷却系统。 MXDC 定期测量同步加速器源晶体的 X 射线衍射数据
用于通过分子置换、重原子和等进行结构测定的单波长和多波长
反常色散相位。 MXDC 的计算资源也可用于 X 射线数据
小分子和大分子的处理、结构测定、精化和可视化
结构测定。 MXDC 鼓励协作模式,其中教师、学生和工作人员
在核心人员的协助和培训下开展项目。 MXDC 是培训资源,
蛋白质表达/纯化以及小分子和蛋白质表达/纯化方面的教育、咨询和合作
高分子X射线晶体学技术。第二阶段 CBSD 这一部分的具体目标
COBRE 是: 1、通过采用项目程序来监督和维护高效的协作设施
规划、适当的费用结构和项目报告机制,以及短期和战略
在咨询委员会的指导下进行规划; 2、维持和增强基因结构和小
通过采用最先进的技术提供分子结构测定服务; 3、进行研究
与 CBSD COBRE 研究人员在涉及 MXDC 领域的项目方面进行合作
专业知识; 4、提供蛋白质表达、纯化、
为 CBSD 研究人员和 UM 提供结晶和 X 射线结构测定(宏观和小分子)
教师通过直接合作、研讨会、讲座、MXDC 网站和 MDXC 核心奖学金
程序。
1
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Stephen R Sprang其他文献
Stephen R Sprang的其他文献
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{{ truncateString('Stephen R Sprang', 18)}}的其他基金
Mechanism of G protein Activation by Ric-8A - competitive revision of R01GM105993
Ric-8A 激活 G 蛋白的机制 - R01GM105993 的竞争性修订版
- 批准号:
8960270 - 财政年份:2013
- 资助金额:
$ 23.07万 - 项目类别:
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